Invitrogen

PSIP1 Monoclonal Antibody (E.493.5)

PSIP1 Antibody in Immunocytochemistry (ICC/IF)

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PSIP1 Antibody (MA5-14821) in ICC/IF

Immunofluorescence analysis of PSIP was performed using SH-SY5Y and differentiated SH-SY5Y cells (Retinoic acid 10 uM, 8 Days). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSIP Rabbit Monoclonal Antibody (E.493.5) (Product # MA5-14821) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibod... View More

PSIP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PSIP1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells and tissues owing to their inherent genetic constitution. Lowered expression of PSIP1 was observed in differentiated SH-SY5Y (Retinoic acid treatment) cells as compared to undifferentiated SH-SY5Y cells using PSIP1 Monoclonal antibody product (Product # MA5-14821) in immunofluorescence. {RE}

Product Details

MA5-14821

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Immunocytochemistry (ICC/IF)

1:250-1:400

Flow Cytometry (Flow)

1:100

ChIP assay (ChIP)

10 µL/10^6 cells

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

E.493.5

Immunogen

Synthetic peptide corresponding to amino acids surrounding Lys461 of human LEDGF

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

9 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11009140

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SETD2 Haploinsufficiency Enhances Germinal Center-Associated AICDA Somatic Hypermutation to Drive B-cell Lymphomagenesis.

Cancer discovery

Leung W,Teater M,Durmaz C,Meydan C,Chivu AG,Chadburn A,Rice EJ,Muley A,Camarillo JM,Arivalagan J,Li Z,Flowers CR,Kelleher NL,Danko CG,Imielinski M,Dave SS,Armstrong SA,Mason CE,Melnick AM

MA5-14821 was used in ChIP Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to define a B cell-specific oncogenic effect of SETD2 heterozygous mutation, which unleashes AICDA mutagenesis of nontemplate strand DNA in the GC reaction, resulting in lymphomas with heavy mutational burden.

Wed Jul 06 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: CLL-associated antigen KW-7; dense fine speckles 70; Dense fine speckles 70 kDa protein; DFS 70; Lens epithelium-derived growth factor; mLEDGF; PC4 and SFRS1 interacting protein 2; PC4 and SFRS1-interacting protein; supercoiled DNA binding protein 75; transcriptional coactivator p52/p75; Transcriptional coactivator p75/p52

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Gene Aliases: AA408851; AU015605; DFS70; LEDGF; Ledgfa; Ledgfb; p52; p75; PAIP; PSIP1; PSIP2; SBP75

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UniProt ID: (Human) O75475, (Mouse) Q99JF8, (Rat) Q812D1

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Entrez Gene ID: (Human) 11168, (Mouse) 101739, (Rat) 313323

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Function(s)

protein binding poly(A) RNA binding supercoiled DNA binding RNA polymerase II transcription coactivator activity DNA binding chromatin binding activating transcription factor binding

Process(es)

mRNA 5'-splice site recognition transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter response to oxidative stress response to heat positive regulation of transcription from RNA polymerase II promoter nuclear transport establishment of integrated proviral latency

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PSIP1 Monoclonal Antibody (E.493.5)

PSIP1 Antibody (MA5-14821) in ICC/IF

Product Details

MA5-14821

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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