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Invitrogen
PSMA5 Polyclonal Antibody
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FIGURE: 1 / 7
PSMA5 Antibody (PA1-1962) in ICC/IF
Immunofluorescence analysis of Proteasome 20S Subunit alpha-5 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSMA5 Rabbit Polyclonal (Product # PA1-1962) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj... View More
Product Details
PA1-1962
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C A(120) L Q F G E E D A D P G A M(133) of human proteasome 20S subunit alpha 5.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2171717
Product Specific Information
PA1-1962 detects proteasome 20S subunit alpha 5 from human, rat, mouse and hamster cell lysates.
PA1-1962 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a 26.5 kDa protein representing proteasome 20S subunit alpha 5 from human, rat, mouse, and hamster cell extracts.
PA1-1962 immunogen is a synthetic peptide corresponding to residues C A(120) L Q F G E E D A D P G A M(133) of human proteasome 20S subunit alpha 5. PA1-1962 immunizing peptide (Cat. # PEP-210) is available for use in neutralization and control experiments.
Target Information
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha-5; epididymis tissue sperm binding protein Li 11n; macropain subunit zeta; Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; proteasome (prosome, macropain) subunit, alpha type, 5; proteasome alpha 5 subunit; proteasome component 5; Proteasome subunit alpha type-5; proteasome subunit alpha type-5-like protein; Proteasome subunit alpha-5; proteasome subunit zeta; Proteasome zeta chain
Gene Aliases: PSC5; PSMA5; ZETA
UniProt ID: (Human) P28066, (Mouse) Q9Z2U1
Entrez Gene ID: (Human) 5686, (Mouse) 26442, (Chinese hamster) 100774039, (Rat) 29672
proteolysis
ubiquitin-dependent protein catabolic process
proteasomal ubiquitin-independent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
proteolysis involved in cellular protein catabolic process
MAPK cascade
protein polyubiquitination
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
regulation of cellular amino acid metabolic process
anaphase-promoting complex-dependent catabolic process
tumor necrosis factor-mediated signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
regulation of mRNA stability
T cell receptor signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
Wnt signaling pathway, planar cell polarity pathway
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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