Invitrogen

PSMB6 Polyclonal Antibody

PSMB6 Antibody in Immunocytochemistry (ICC/IF)

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PSMB6 Antibody (PA1-978) in ICC/IF

Immunofluorescent analysis of Proteasome 20S Y (green) in 3T3 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 30 minutes at room temperature. Cells were stained with or without Proteasome 20S Y rabbit polyclonal antibody (Product # PA1-978), at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor... View More

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PSMB6 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. PC-3 cells were transfected with PSMB6 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-PSMB6 Polyclonal Antibody (Product # PA1-978). {KD}

Product Details

PA1-978

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptides: C(41) R S G S A A D T Q A V/I A D A V T Y(58)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2172197

Product Specific Information

PA1-978 detects proteasome 20S Y from purified bovine and human 26S proteasome samples.

PA1-978 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~25 kDa protein representing proteasome 20S Y from purified human 26S proteasome.

The PA1-978 immunizing peptides correspond to amino acid residues 41-58 from human proteasome 20S Y and amino acid residues 74-91 from frog. These sequences are completely conserved in mouse, rat, and zebrafish. PA1-978 immunizing peptide (Cat. # PEP-173) is available for use in neutralization and control procedures.

Target Information

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signaling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. This complex, consisting of alpha, beta, and gamma subunits, enhances the activity of the 20S proteolytic core. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator), Angelmane's syndrome (E6-AP), and Liddle syndrome (endothelial sodium channels).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Non-functional ubiquitin C-terminal hydrolase L1 drives podocyte injury through impairing proteasomes in autoimmune glomerulonephritis.

Nature communications

Reichelt J,Sachs W,Frömbling S,Fehlert J,Studencka-Turski M,Betz A,Loreth D,Blume L,Witt S,Pohl S,Brand J,Czesla M,Knop J,Florea BI,Zielinski S,Sachs M,Hoxha E,Hermans-Borgmeyer I,Zahner G,Wiech T,Krüger E,Meyer-Schwesinger C

PA1-978 was used in Western Blotting to investigate the mechanistic significance of the ubiquitin proteasome system (UPS) in membranous nephropathy (MN) and to uncover that the deubiquitinase Ubiquitin C-terminal hydrolase L1 (UCH-L1) is induced by oxidative stress in podocytes and directly involved in proteasome substrate accumulation, leading to podocyte injury and proteinuria.

Thu Apr 13 00:00:00 UTC 2023

Evaluation of Immunoproteasome-Specific Proteolytic Activity Using Fluorogenic Peptide Substrates.

Immune network

Kim S,Park SH,Choi WH,Lee MJ

PA1-978 was used in Western Blotting to show that the most accurate assessment of immunoproteasome activity could be achieved by combining β5i-targeting substrate Ac-ANW-AMC and immunoproteasome inhibitor ONX-0914.

Wed Jun 01 00:00:00 UTC 2022

The ubiquitin-like protein FAT10 mediates NF-kappaB activation.

Journal of the American Society of Nephrology : JASN

Gong P,Canaan A,Wang B,Leventhal J,Snyder A,Nair V,Cohen CD,Kretzler M,D'Agati V,Weissman S,Ross MJ

PA1-978 was used in western blot to investigate the regulatory effect of ubiquitin-like protein FAT10 on NF-kappa B activation

Mon Feb 01 00:00:00 UTC 2010

Persistent hijacking of brain proteasomes in HIV-associated dementia.

The American journal of pathology

Nguyen TP,Soukup VM,Gelman BB

PA1-978 was used in western blot to study the effect of HIV infection on brain proteasomes and its role in dementia

Mon Feb 01 00:00:00 UTC 2010

Reduced ubiquitin C-terminal hydrolase-1 expression levels in dementia with Lewy bodies.

Neurobiology of disease

Barrachina M,Castaño E,Dalfó E,Maes T,Buesa C,Ferrer I

PA1-978 was used in western blot to investigate the changes of UCHL-1 in samples from dementia patients with Lewy bodies

Mon May 01 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: beta-1; Low molecular mass protein 19; Macropain delta chain; Multicatalytic endopeptidase complex delta chain; proteasome (prosome, macropain) subunit, beta type, 6; proteasome beta 6 subunit; proteasome catalytic subunit 1; Proteasome chain 5; Proteasome delta chain; Proteasome subunit beta type-6; Proteasome subunit beta-1; proteasome subunit delta; Proteasome subunit Y; PSY large multifunctional protease Y; RP23-122P1.4; subunit 2

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Gene Aliases: BOS_18680; DELTA; Lmp19; LMPY; Mpnd; PSMB6; Psmb6l; Y

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UniProt ID: (Human) P28072, (Bovine) Q3MHN0, (Rat) P28073, (Mouse) Q60692

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Entrez Gene ID: (Human) 5694, (Bovine) 510069, (Rat) 29666, (Mouse) 19175

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Function(s)

endopeptidase activity threonine-type endopeptidase activity cadherin binding involved in cell-cell adhesion peptidase activity hydrolase activity

Process(es)

MAPK cascade protein polyubiquitination stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent regulation of cellular amino acid metabolic process viral process anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability T cell receptor signaling pathway negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway cell-cell adhesion proteolysis involved in cellular protein catabolic process proteolysis proteasomal ubiquitin-independent protein catabolic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PSMB6 Polyclonal Antibody

PSMB6 Antibody (PA1-978) in ICC/IF

Product Details

PA1-978

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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