Invitrogen

PSMB8 Polyclonal Antibody

PSMB8 Antibody in Immunocytochemistry (ICC/IF)

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PSMB8 Antibody (PA1-972) in ICC/IF

Immunofluorescent analysis of Proteasome 20S LMP7 (green) in HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) in PBS for 15 minutes at room temperature. Cells were stained with or without Proteasome 20S LMP7 rabbit polyclonal antibody (Product # PA1-972), at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with a Alexa Fluor® 488 Superclonal goat anti-rabbit IgG secondary antibo... View More

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PSMB8 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot of PSMB8 using PSMB8 Polyclonal Antibody (Product # PA1-972), shows increased expression of PSMB8 in HeLa cell line upon IFN-gamma treatment. {TM}

Product Details

PA1-972

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C V(259) E S T D V S D L L H Q Y R E A(274)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2172350

Product Specific Information

PA1-972 detects Proteasome 20S LMP7 in western blot and immunofluorescence.

PA1-972 can be used with blocking peptide PEP-132.

Target Information

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Evaluation of Immunoproteasome-Specific Proteolytic Activity Using Fluorogenic Peptide Substrates.

Immune network

Kim S,Park SH,Choi WH,Lee MJ

PA1-972 was used in Western Blotting to show that the most accurate assessment of immunoproteasome activity could be achieved by combining β5i-targeting substrate Ac-ANW-AMC and immunoproteasome inhibitor ONX-0914.

Wed Jun 01 00:00:00 UTC 2022

Human Lentiviral Gene Therapy Restores the Cellular Phenotype of Autosomal Recessive Complete IFN-γR1 Deficiency.

Molecular therapy. Methods & clinical development

Hahn K,Pollmann L,Nowak J,Nguyen AHH,Haake K,Neehus AL,Waqas SFH,Pessler F,Baumann U,Hetzel M,Casanova JL,Schulz A,Bustamante J,Ackermann M,Lachmann N

PA1-972 was used in Western Blotting to highlight lentiviral vectors to correct the IFN-γ mediated immunity and present the first gene therapy approach for patients suffering from AR complete IFN-γR1 deficiency.

Fri Jun 12 00:00:00 UTC 2020

Human Lentiviral Gene Therapy Restores the Cellular Phenotype of Autosomal Recessive Complete IFN-γR1 Deficiency.

Molecular therapy. Methods & clinical development

Hahn K,Pollmann L,Nowak J,Nguyen AHH,Haake K,Neehus AL,Waqas SFH,Pessler F,Baumann U,Hetzel M,Casanova JL,Schulz A,Bustamante J,Ackermann M,Lachmann N

Fri Jun 12 00:00:00 UTC 2020

Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics.

Cell reports

Markmiller S,Fulzele A,Higgins R,Leonard M,Yeo GW,Bennett EJ

PA1-972 was used in Immunocytochemistry-immunoflourescence to study whether active ubiquitylation or neddylation modulates stress granule dynamics.

Tue Apr 30 00:00:00 UTC 2019

Glucosamine induces cell death via proteasome inhibition in human ALVA41 prostate cancer cell.

Experimental & molecular medicine

Liu BQ,Meng X,Li C,Gao YY,Li N,Niu XF,Guan Y,Wang HQ

PA1-972 was used in western blot to investigate the mechanism for glucosamine-induced cell apoptosis in cancer cells

Fri Sep 30 00:00:00 UTC 2011

Inhibition of apoptosis in acute promyelocytic leukemia cells leads to increases in levels of oxidized protein and LMP2 immunoproteasome.

Proceedings of the National Academy of Sciences of the United States of America

Khan MA,Oubrahim H,Stadtman ER

PA1-972 was used in western blot to study the mechanism for the observed accumulation of oxidized proteins during aging and in age-related diseases.

Tue Aug 10 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: DAAP-57C1.3; Low molecular mass protein 7; low molecular weight protein 7; Macropain subunit C13; MGC1491; Multicatalytic endopeptidase complex subunit C13; OTTHUMP00000029420; OTTHUMP00000029421; OTTHUMP00000062981; protease component C13; proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7); proteasome catalytic subunit 3i; Proteasome component C13; proteasome subunit beta 5i; Proteasome subunit beta type-8; Proteasome subunit beta-5i; proteasome subunit Y2; proteasome-related gene 7; Really interesting new gene 10 protein

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Gene Aliases: ALDD; D6S216; D6S216E; JMP; LMP7; NKJO; PSMB5i; PSMB8; RING10; Y2

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UniProt ID: (Human) P28062

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Entrez Gene ID: (Human) 5696

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Function(s)

threonine-type endopeptidase activity protein binding

Process(es)

MAPK cascade protein polyubiquitination stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent regulation of cellular amino acid metabolic process viral process anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability fat cell differentiation T cell receptor signaling pathway negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of endopeptidase activity Wnt signaling pathway, planar cell polarity pathway type I interferon signaling pathway negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PSMB8 Polyclonal Antibody

PSMB8 Antibody (PA1-972) in ICC/IF

Product Details

PA1-972

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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