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PSMC5 Antibody (MA3-087) in ICC/IF
Immunofluorescent analysis of SUG1 (green) in U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a SUG1 mouse monoclonal antibody (Product # MA3-087) at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal™ Secondary Antibody, Alexa Fluor 488 conjugate (Product # A28175) at a ... View More
Product Details
MA3-087
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2SU-1B8
Immunogen
Recombinant Mouse SUG1 aa 1 to 149
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2633332
Product Specific Information
MA3-087 detects SUG1 from human, Non-human primate, mouse and rat samples.
MA3-087 has been successfully used in Western blot, immunofluorescence, Flow Cytometry, and ELISA applications.
Target Information
Human SUG1 (also known as p45, Rpt6 and Cim3) is a component of the 19S regulatory subunit of the 26S proteosome complex. It is one of the AAA ATPas found in the 19S regulatory complex that is thought to participate in the unfolding of ubiquitinated proteins in an ATP-dependent manner. It has also been shown to directly interact with the nucleotide excision repair protein XPB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 26S protease regulatory subunit 8; 26S proteasome AAA-ATPase subunit RPT6; 26S proteasome regulatory subunit 8; for proteasomal ATPase (SUG1); mSUG1; MSUG1 protein; p45/SUG; peptidase (prosome, macropain) 26S subunit, ATPase 5; protease (prosome, macropain) 26S subunit, ATPase 5; proteasomal ATPase (SUG1); proteasome (prosome, macropain) 26S subunit, ATPase, 5; proteasome 26S ATPase subunit 5; Proteasome 26S subunit ATPase 5; proteasome p45/SUG; Proteasome subunit p45; Tat-binding protein homolog 10; testicular tissue protein Li 149; thy; Thyroid hormone receptor-interacting protein 1; thyroid receptor interactor 1; TRIP1
Gene Aliases: mSUG1; p45; p45/SUG; PSMC5; S8; SUG-1; SUG1; TBP10; TRIP1
UniProt ID: (Human) A8K3Z3, (Mouse) P62196, (Rat) P62198
Entrez Gene ID: (Human) 5705, (Mouse) 19184, (Rat) 81827
protein catabolic process
ER-associated ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of transcription, DNA-templated
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of inclusion body assembly
positive regulation of proteasomal protein catabolic process
MAPK cascade
protein polyubiquitination
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
transcription from RNA polymerase II promoter
regulation of cellular amino acid metabolic process
anaphase-promoting complex-dependent catabolic process
tumor necrosis factor-mediated signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
negative regulation of programmed cell death
regulation of mRNA stability
positive regulation of transcription, DNA-templated
T cell receptor signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
Wnt signaling pathway, planar cell polarity pathway
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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