Invitrogen

PSMD1 Polyclonal Antibody

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PSMD1 Antibody (PA1-973) in WB

Western blot analysis was performed on whole cell extracts (30 µglysate) of MCF7 (Lane 1), PC-3 (Lane 2), Hep G2 (Lane 3), SK-OV-3 (Lane 4), HeLa (Lane 5), COS-7 (Lane 6), Jurkat (Lane 7), A-431 (Lane 8) and tissue extracts (30 µglysate) of Mouse Testis (Lane 9) and Rat Testis (Lane 10). The blot was probed with Anti-PSMD1 Rabbit Polyclonal Antibody (Product # PA1-973, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilut... View More

Product Image Gallery

PSMD1 Antibody — IP-MS enrichment of PSMD1 (LFQ intensity): PSMD1 was enriched 321-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and PSMD1 antibody (Product # PA1-973). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

PA1-973

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

15 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: A(929) A H G P K I E E E E Q E P E P P E P F E Y I D D(953)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2172804

Product Specific Information

PA1-973 detects proteasome 19S subunit S1 from from human cells.

PA1-973 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~105 kDa protein representing proteasome 19S subunit S1 from HeLa cell extract.

PA1-973 immunizing peptide corresponds to amino acid residues 929-953 from human proteasome 19S subunit S1 protein.

Target Information

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman’s syndrome, and Liddle syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Impairments in age-dependent ubiquitin proteostasis and structural integrity of selective neurons by uncoupling Ran GTPase from the Ran-binding domain 3 of Ranbp2 and identification of novel mitochondrial isoforms of ubiquitin-conjugating enzyme E2I (ubc9) and Ranbp2.

Small GTPases

Patil H,Yoon D,Bhowmick R,Cai Y,Cho KI,Ferreira PA

PA1-973 was used in Western Blotting to support that Ran GTPase-dependent and independent and moonlighting roles of Ranbp2 or domains thereof and ubc9 control selectively age-dependent, neural-type and mitochondrial functions.

Fri Mar 01 00:00:00 UTC 2019

Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells.

PloS one

Chadchankar J,Korboukh V,Conway LC,Wobst HJ,Walker CA,Doig P,Jacobsen SJ,Brandon NJ,Moss SJ,Wang Q

PA1-973 was used in Western Blotting to study the effects of USP14 inhibition in mammalian cells using small molecule inhibitors and an inactive USP14 mutant C114A.

Mon Mar 23 00:00:00 UTC 2020

Differential loss of prolyl isomerase or chaperone activity of Ran-binding protein 2 (Ranbp2) unveils distinct physiological roles of its cyclophilin domain in proteostasis.

The Journal of biological chemistry

Cho KI,Patil H,Senda E,Wang J,Yi H,Qiu S,Yoon D,Yu M,Orry A,Peachey NS,Ferreira PA

Published figure using PSMD1 polyclonal antibody (Product # PA1-973) in Western Blot

Fri Feb 21 00:00:00 UTC 2014

Glucosamine induces cell death via proteasome inhibition in human ALVA41 prostate cancer cell.

Experimental & molecular medicine

Liu BQ,Meng X,Li C,Gao YY,Li N,Niu XF,Guan Y,Wang HQ

PA1-973 was used in western blot to investigate the mechanism for glucosamine-induced cell apoptosis in cancer cells

Fri Sep 30 00:00:00 UTC 2011

Persistent hijacking of brain proteasomes in HIV-associated dementia.

The American journal of pathology

Nguyen TP,Soukup VM,Gelman BB

PA1-973 was used in western blot to study the effect of HIV infection on brain proteasomes and its role in dementia

Mon Feb 01 00:00:00 UTC 2010

Reduced ubiquitin C-terminal hydrolase-1 expression levels in dementia with Lewy bodies.

Neurobiology of disease

Barrachina M,Castaño E,Dalfó E,Maes T,Buesa C,Ferrer I

PA1-973 was used in western blot to investigate the changes of UCHL-1 in samples from dementia patients with Lewy bodies

Mon May 01 00:00:00 UTC 2006

Neuropathology and pathogenesis of encephalitis following amyloid-beta immunization in Alzheimer's disease.

Brain pathology (Zurich, Switzerland)

Ferrer I,Boada Rovira M,Sánchez Guerra ML,Rey MJ,Costa-Jussá F

PA1-973 was used in immunohistochemistry to investigate the cause of meningoencephalitis after immunization with amyloid-beta peptide in Alzheimer disease

Thu Jan 01 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: 26S proteasome non-ATPase regulatory subunit 1; 26S proteasome p112 subunit; 26S proteasome regulatory subunit RPN2; 26S proteasome regulatory subunit S1; 26S proteasome subunit p112; 26S proteasome, subunit p112; MGC133040; MGC133041; proteasome (prosome, macropain) 26S subunit, non-ATPase, 1; proteasome 26S non-ATPase subunit 1

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Gene Aliases: 2410026J11Rik; BOS_2177; P112; PSMD1; Rpn2; S1

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UniProt ID: (Human) Q99460, (Mouse) Q3TXS7, (Rat) O88761

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Entrez Gene ID: (Human) 5707, (Bovine) 511803, (Mouse) 70247, (Rat) 83806

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Function(s)

endopeptidase activity enzyme regulator activity protein binding

Process(es)

MAPK cascade protein polyubiquitination stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent regulation of cellular amino acid metabolic process anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability T cell receptor signaling pathway negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway regulation of protein catabolic process regulation of catalytic activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PSMD1 Polyclonal Antibody

PSMD1 Antibody (PA1-973) in WB

Product Details

PA1-973

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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