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Invitrogen
PSMD13 Polyclonal Antibody
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FIGURE: 1 / 4
PSMD13 Antibody (PA5-144662) in ICC/IF
Immunocytochemistry analysis of PSMD13 in HepG2 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with PSMD13 polyclonal antibody (Product # PA5-144662) with a dilution of 1:200 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary an... View More
Product Details
PA5-144662
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within Human PSMD13 aa 1-160/376.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3094047
Product Specific Information
Positive control: HepG2 cell lysate, Hela cell lysate, Siha cell lysate, SH-SY5Y cell lysate, NIH3T3 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, Siha, HepG2, Hela.
Predicted band size: 43 kDa
Subcellular Location: cytosol, extracellular region, nucleoplasm, nucleus.
Target Information
Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 26S proteasome non-ATPase regulatory subunit 13; 26S proteasome non-ATPase regulatory subunit 13 {ECO:0000250|UniProtKB:Q9UNM6, ECO:0000312|EMBL:AAI58733.1}; 26S proteasome regulatory subunit p40.5; 26S proteasome regulatory subunit p40.5 {ECO:0000250|UniProtKB:Q9UNM6}; 26S proteasome regulatory subunit RPN9; 26S proteasome regulatory subunit S11; 26S proteasome regulatory subunit S11 {ECO:0000250|UniProtKB:Q9UNM6}; 26S proteasome subunit p40.5; proteasome (prosome, macropain) 26S subunit, non-ATPase, 13; psmd13 {ECO:0000312|EMBL:AAI58733.1, ECO:0000312|RGD:1305236}
Gene Aliases: HSPC027; p40.5; PSMD13; Rpn9; S11
UniProt ID: (Human) Q9UNM6, (Mouse) Q9WVJ2, (Rat) B0BN93
Entrez Gene ID: (Human) 5719, (Mouse) 23997, (Rat) 365388
proteolysis
meiosis I
MAPK cascade
protein polyubiquitination
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
ubiquitin-dependent protein catabolic process
regulation of cellular amino acid metabolic process
anaphase-promoting complex-dependent catabolic process
tumor necrosis factor-mediated signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
proteasome-mediated ubiquitin-dependent protein catabolic process
proteasome assembly
regulation of mRNA stability
T cell receptor signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
Wnt signaling pathway, planar cell polarity pathway
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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