Proteintech

PSMD14/POH1 Polyclonal Antibody

PSMD14/POH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PSMD14/POH1 Antibody (12059-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human colon cancer using 12059-1-AP (PSMD14/POH1 antibody) at dilution of 1:50 (under 10x lens).

PSMD14/POH1 Antibody in Western Blot (WB)

Product Image Gallery

Product Details

12059-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PSMD14/POH1 Fusion Protein Ag2694 (1-95 aa encoded by BC009524)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MLLNLHKKS WMEGLTLQDY SEHCKHNESV VKEMLELAKN YNKAVEEEDK MTPEQLAIKN VGKQDPKRHL EEHVDVLMTS NIVQCLAAML DTVVFK (1-95 aa encoded by BC009524)

Target Information

The 26S proteasome is responsible for most intracellular proteolysis in eukaryotes. POH1 (also known as Rpn11 in yeast) subunit of the 19S complex is responsible for substrate deubiquitination during proteasomal degradation. POH1 appears to be a Zn21-dependent protease, unlike all other known deubiquitinating enzymes (DUBs) that are cysteine proteases. POH1 was first identified as the human functional homolog of the S. pombe pad1 gene, which confers moderate resistance to chemotherapeutic drugs, and also to ultraviolet light when transiently overproduced in mammalian cells. Poh1-induced drug resistance is mediated through AP-1 transcription factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Depletion of PSMD14 suppresses bladder cancer proliferation by regulating GPX4.

PeerJ

Jia C,Zhang X,Qu T,Wu X,Li Y,Zhao Y,Sun L,Wang Q

Wed Feb 15 00:00:00 UTC 2023

Depletion of PSMD14 suppresses bladder cancer proliferation by regulating GPX4.

PeerJ

Jia C,Zhang X,Qu T,Wu X,Li Y,Zhao Y,Sun L,Wang Q

Wed Feb 15 00:00:00 UTC 2023

Deubiquitylase PSMD14 inhibits autophagy to promote ovarian cancer progression via stabilization of LRPPRC.

Biochimica et biophysica acta. Molecular basis of disease

Zhao Z,Xu H,Wei Y,Sun L,Song Y

Wed Feb 01 00:00:00 UTC 2023

Deubiquitylase PSMD14 inhibits autophagy to promote ovarian cancer progression via stabilization of LRPPRC.

Biochimica et biophysica acta. Molecular basis of disease

Zhao Z,Xu H,Wei Y,Sun L,Song Y

Wed Feb 01 00:00:00 UTC 2023

The PSMD14 inhibitor Thiolutin as a novel therapeutic approach for esophageal squamous cell carcinoma through facilitating SNAIL degradation.

Theranostics

Jing C,Li X,Zhou M,Zhang S,Lai Q,Liu D,Ye B,Li L,Wu Y,Li H,Yue K,Chen P,Yao X,Wu Y,Duan Y,Wang X

Fri Jul 30 00:00:00 UTC 2021

The PSMD14 inhibitor Thiolutin as a novel therapeutic approach for esophageal squamous cell carcinoma through facilitating SNAIL degradation.

Theranostics

Jing C,Li X,Zhou M,Zhang S,Lai Q,Liu D,Ye B,Li L,Wu Y,Li H,Yue K,Chen P,Yao X,Wu Y,Duan Y,Wang X

Fri Jul 30 00:00:00 UTC 2021

Blockade of deubiquitinating enzyme PSMD14 overcomes chemoresistance in head and neck squamous cell carcinoma by antagonizing E2F1/Akt/SOX2-mediated stemness.

Theranostics

Jing C,Duan Y,Zhou M,Yue K,Zhuo S,Li X,Liu D,Ye B,Lai Q,Li L,Yao X,Wei H,Zhang W,Wu Y,Wang X

Tue Jul 27 00:00:00 UTC 2021

MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma.

Clinical and translational medicine

Xiao M,Liu J,Xiang L,Zhao K,He D,Zeng Q,Zhang Q,Xie D,Deng M,Zhu Y,Zhang Y,Liu Y,Bo H,Liu X,Chen X,Gong L,Bao Y,Hu Y,Cheng Y,Deng L,Zhu R,Xing X,Zhou M,Xiong W,Zhou Y,Zhou J,Li X,Cao K

Tue Dec 01 00:00:00 UTC 2020

Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa.

Science translational medicine

Byrd AS,Carmona-Rivera C,O'Neil LJ,Carlucci PM,Cisar C,Rosenberg AZ,Kerns ML,Caffrey JA,Milner SM,Sacks JM,Aliu O,Broderick KP,Reichner JS,Miller LS,Kang S,Robinson WH,Okoye GA,Kaplan MJ

Wed Sep 04 00:00:00 UTC 2019

Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa.

Science translational medicine

Byrd AS,Carmona-Rivera C,O'Neil LJ,Carlucci PM,Cisar C,Rosenberg AZ,Kerns ML,Caffrey JA,Milner SM,Sacks JM,Aliu O,Broderick KP,Reichner JS,Miller LS,Kang S,Robinson WH,Okoye GA,Kaplan MJ

Wed Sep 04 00:00:00 UTC 2019

Computational and biochemical studies of isothiocyanates as inhibitors of proteasomal cysteine deubiquitinases in human cancer cells.

Journal of cellular biochemistry

Ahmed ZSO,Li X,Li F,Cheaito HA,Patel K,Mosallam EM,Elbargeesy GAEH,Dou QP

Thu Nov 01 00:00:00 UTC 2018

Computational and biochemical studies of isothiocyanates as inhibitors of proteasomal cysteine deubiquitinases in human cancer cells.

Journal of cellular biochemistry

Ahmed ZSO,Li X,Li F,Cheaito HA,Patel K,Mosallam EM,Elbargeesy GAEH,Dou QP

Thu Nov 01 00:00:00 UTC 2018

RPN11 deubiquitinase promotes proliferation and migration of breast cancer cells.

Molecular medicine reports

Luo G,Hu N,Xia X,Zhou J,Ye C

Sat Jul 01 00:00:00 UTC 2017

RPN11 deubiquitinase promotes proliferation and migration of breast cancer cells.

Molecular medicine reports

Luo G,Hu N,Xia X,Zhou J,Ye C

Sat Jul 01 00:00:00 UTC 2017

POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.

Nature communications