Invitrogen

PSME1 Polyclonal Antibody

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PSME1 Antibody (PA1-960) in WB

Western blot on PA28 alpha in Hepa 1 cell lysate using Product # PA1-960.

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PSME1 Antibody — IP-MS enrichment of PSME1 (LFQ intensity): PSME1 was enriched 22-fold from A549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and PSME1 antibody (Product # PA1-960). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

PA1-960

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 5 publications 5 publications

Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: R(5) V Q P E A Q A K V D V F R E D L C(22)

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2284415

Product Specific Information

PA1-960 detects proteasome activator 11S REG alpha subunit from canine, hamster, human, mouse and rat tissues and cells.

PA1-960 has been successfully used in Western blot procedures. By Western blot, this antibody detects a 28 kDa protein representing 11S REG alpha subunit in rat brain extract.

PA1-960 immunizing peptide corresponds to amino acid residues 5-22 of human proteasome activator 11S REG alpha. This sequence differs from the mouse and rat 11S REG alpha protein by a single amino acid residue. PA1-960 immunizing peptide (Cat. # PEP-097) is available for use in neutralization and control experiments.

Target Information

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (approximately 31), barrel-shaped molecular machine with an apparent molecular weight of approximately 2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Heterogeneous nuclear ribonucleoprotein A2 participates in the replication of Japanese encephalitis virus through an interaction with viral proteins and RNA.

Journal of virology

Katoh H,Mori Y,Kambara H,Abe T,Fukuhara T,Morita E,Moriishi K,Kamitani W,Matsuura Y

PA1-960 was used in western blot to investigate the interaction between heterogeneous nuclear ribonucleoprotein A2 and the Japanese encephalitis virus core protein and its involvement in viral replication

Tue Nov 01 00:00:00 UTC 2011

Characterization of the Brain 26S Proteasome and its Interacting Proteins.

Frontiers in molecular neuroscience

Tai HC,Besche H,Goldberg AL,Schuman EM

PA1-960 was used in western blot to detect the change in the proteasome-associated protein PA28alpha after NMDA exposure

Thu Jul 14 00:00:00 UTC 2011

Alterations of cerebral cortex and hippocampal proteasome subunit expression and function in a traumatic brain injury rat model.

Journal of neurochemistry

Yao X,Liu J,McCabe JT

PA1-960 was used in western blot to study the role of proteasome in a traumatic brain injury

Tue Jan 01 00:00:00 UTC 2008

Reduced ubiquitin C-terminal hydrolase-1 expression levels in dementia with Lewy bodies.

Neurobiology of disease

Barrachina M,Castaño E,Dalfó E,Maes T,Buesa C,Ferrer I

PA1-960 was used in western blot to investigate the changes of UCHL-1 in samples from dementia patients with Lewy bodies

Mon May 01 00:00:00 UTC 2006

Proteasome impairment does not contribute to pathogenesis in R6/2 Huntington's disease mice: exclusion of proteasome activator REGgamma as a therapeutic target.

Human molecular genetics

Bett JS,Goellner GM,Woodman B,Pratt G,Rechsteiner M,Bates GP

PA1-960 was used in western blot to investigate the role of the proteasome during the pathogenesis of R6/2 Huntington's disease.

Sun Jan 01 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: 11S regulator complex subunit alpha; 29-kD MCP activator subunit; Activator of multicatalytic protease subunit 1; epididymis secretory sperm binding protein Li 129m; IGUP I-5111; Interferon gamma up-regulated I-5111 protein; interferon-gamma IEF SSP 5111; interferon-gamma-inducible protein 5111; MGC8628; OTTHUMP00000164702; PA28 alpha subunit; PA28a; PA28alpha; protease (prosome, macropain) 28 subunit, alpha; proteasome (prosome, macropain) 28 subunit, alpha; proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); Proteasome activator 28 subunit alpha; Proteasome activator complex subunit 1; REG-alpha

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Gene Aliases: AW413925; HEL-S-129m; I79_008549; IFI5111; PA28A; PA28alpha; PSME1; REGalpha

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UniProt ID: (Human) Q06323, (Mouse) P97371

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Entrez Gene ID: (Human) 5720, (Dog) 480256, (Mouse) 19186, (Chinese hamster) 100761736, (Rat) 29630

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Function(s)

endopeptidase activator activity protein binding

Process(es)

positive regulation of endopeptidase activity antigen processing and presentation of exogenous antigen MAPK cascade protein polyubiquitination stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent regulation of cellular amino acid metabolic process anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability T cell receptor signaling pathway negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway regulation of proteasomal protein catabolic process negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway regulation of G1/S transition of mitotic cell cycle

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PSME1 Polyclonal Antibody

PSME1 Antibody (PA1-960) in WB

Product Details

PA1-960

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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