Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
PSME2 Polyclonal Antibody
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 1
PSME2 Antibody (PA5-120231) in WB
Western blot analysis of PSME2 in extracts from wild type (WT) and PSME2 knockout (KO) 293T(KO) cells. Samples were incubated with PSME2 Polyclonal antibody (Product # PA5-120231) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-120231
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-239 of human PSME2 (NP_002809.2).
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913803
Product Specific Information
Positive test controls include: U-251MG, Mouse thymus, Mouse lung, Mouse kidney.
Immunogen sequence: MAKPCGVRLS GEARKQVEVF RQNLFQEAEE FLYRFLPQKI IYLNQLLQED SLNVADLTSL RAPLDIPIPD PPPKDDEMET DKQEKKEVHK CGFLPGNEKV LSLLALVKPE VWTLKEKCIL VITWIQHLIP KIEDGNDFGV AIQEKVLERV NAVKTKVEAF QTTISKYFSE RGDAVAKASK ETHVMDYRAL VHERDEAAYG ELRAMVLDLR AFYAELYHII SSNLEKIVNP KGEEKPSMY
Target Information
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (approximately 31), barrel-shaped molecular machine with an apparent molecular weight of approximately 2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 11S regulator complex beta subunit; 11S regulator complex subunit beta; Activator of multicatalytic protease subunit 2; cell migration-inducing protein 22; MCP activator, 31-kD subunit; PA28b; PA28beta; protease (prosome, macropain) 28 subunit, beta; proteasome (prosome, macropain) 28 subunit, beta; proteasome (prosome, macropain) activator subunit 2 (PA28 beta); Proteasome activator 28 subunit beta; proteasome activator 28-beta; Proteasome activator complex subunit 2; proteasome activator hPA28 subunit beta; REG-beta
Gene Aliases: AA589371; AI788882; PA28B; Pa28b1; PA28beta; PSME2; REGbeta
UniProt ID: (Human) Q9UL46, (Mouse) P97372
Entrez Gene ID: (Human) 5721, (Mouse) 19188
positive regulation of endopeptidase activity
antigen processing and presentation of exogenous antigen
MAPK cascade
protein polyubiquitination
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
regulation of cellular amino acid metabolic process
anaphase-promoting complex-dependent catabolic process
tumor necrosis factor-mediated signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of mRNA stability
T cell receptor signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
Wnt signaling pathway, planar cell polarity pathway
regulation of proteasomal protein catabolic process
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
regulation of G1/S transition of mitotic cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support