Invitrogen

PSME3 Recombinant Rabbit Monoclonal Antibody (20H9L19)

PSME3 Antibody in Immunocytochemistry (ICC/IF)

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PSME3 Antibody (700180) in ICC/IF

Immunofluorescent analysis of PSME3 in HeLa cells using a PSME3 recombinant rabbit monoclonal antibody (Product # 700180) at a dilution of 5 µg/mL in the absence of peptide (top) and presence of immunogenic peptide (bottom), followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Actin was stained with Alexa Fluor 568 Phalloidin (Product # A12380). Hoechst only (blue, left), AF488 signal only (green, middle) and composite image with Phalloidin (right).

PSME3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

PSME3 Antibody — IP-MS enrichment of PSME3 (LFQ intensity): PSME3 was enriched 753-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and PSME3 antibody (Product # 700180). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

700180

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

Immunocytochemistry (ICC/IF)

4-6 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

20H9L19

Immunogen

A peptide corresponding to amino acids 7-20 of P61289.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532292

Product Specific Information

This antibody is predicted to react with canine, chicken, equine, orangutan, Xenopus and zebrafish based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

SFRS10 contains 1 RRM (RNA recognition motif) domain and belongs to the splicing factor SR family. It is a sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PA28γ-20S proteasome is a proteolytic complex committed to degrade unfolded proteins.

Cellular and molecular life sciences : CMLS

Frayssinhes JA,Cerruti F,Laulin J,Cattaneo A,Bachi A,Apcher S,Coux O,Cascio P

700180 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to indicate that global stimulation of the degradation of unfolded proteins may represent a more general feature of PA28? and suggest that this proteasomal activator might play a broader role in the pathway of protein degradation than previously believed.

Thu Dec 16 00:00:00 UTC 2021

Site-specific O-GlcNAcylation of Psme3 maintains mouse stem cell pluripotency by impairing P-body homeostasis.

Cell reports

Pecori F,Kondo N,Ogura C,Miura T,Kume M,Minamijima Y,Yamamoto K,Nishihara S

700180 was used in Immunoprecipitation to identify O-GlcNAcylation of proteasome activator subunit 3 protein as a node of the embryonic stem cell pluripotency network.

Tue Jul 13 00:00:00 UTC 2021

Mutant p53 (p53-R248Q) functions as an oncogene in promoting endometrial cancer by up-regulating REGγ.

Cancer letters

Wang H,Bao W,Jiang F,Che Q,Chen Z,Wang F,Tong H,Dai C,He X,Liao Y,Liu B,Sun J,Wan X

700180 was used in immunohistochemistry and western blot to demonstrate that the p53-R248Q mutant targets the proteasome activator REGgamma to promote endometrial cancer progression

Fri May 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: 11S regulator complex gamma subunit; 11S regulator complex subunit gamma; Activator of multicatalytic protease subunit 3; epididymis secretory protein Li 283; Ki antigen; Ki nuclear autoantigen; PA28 gamma variant 5; PA28g; prosome macropain; proteaseome (prosome, macropain) 28 subunit, 3; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki); Proteasome activator 28 subunit gamma; proteasome activator 28-gamma; Proteasome activator complex subunit 3; REG gamma-3; REG-gamma

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Gene Aliases: AA410043; Ab2-371; AU020960; HEL-S-283; Ki; PA28-gamma; PA28G; PA28gamma; PSME3; REG-GAMMA; REGgamma

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UniProt ID: (Human) Q13172, (Mouse) P61290

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Entrez Gene ID: (Human) 10197, (Mouse) 19192, (Rat) 287716

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Function(s)

p53 binding protein binding identical protein binding endopeptidase activator activity MDM2/MDM4 family protein binding

Process(es)

MAPK cascade protein polyubiquitination stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent regulation of cellular amino acid metabolic process apoptotic process positive regulation of endopeptidase activity anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability T cell receptor signaling pathway negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway regulation of proteasomal protein catabolic process negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway regulation of G1/S transition of mitotic cell cycle negative regulation of extrinsic apoptotic signaling pathway cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PSME3 Recombinant Rabbit Monoclonal Antibody (20H9L19)

PSME3 Antibody (700180) in ICC/IF

Product Details

700180

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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