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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PSPH Antibodies

          Invitrogen

          PSPH Polyclonal Antibody

          Advanced Verification
          5 Published Figures
          3 References
          View all (17) PSPH antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite PSPH Polyclonal Antibody

          • Antibody Testing Data (5)
          • Published Figures (5)
          • Advanced Verification (1)
          PSPH Antibody in Immunocytochemistry (ICC/IF)
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          PSPH Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

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          PSPH Antibody (PA5-22003) in ICC/IF

          Immunofluorescence analysis of PSPH was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSPH Polyclonal Antibody (Product # PA5-22003) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          PSPH Antibody in Immunocytochemistry (ICC/IF)
          PSPH Antibody in Immunocytochemistry (ICC/IF)
          PSPH Antibody in Western Blot (WB)
          PSPH Antibody in Western Blot (WB)
          PSPH Antibody in Western Blot (WB)
          PSPH Antibody in Western Blot (WB)
          PSPH Antibody in Western Blot (WB)
          PSPH Antibody in Western Blot (WB)
          PSPH Antibody in Western Blot (WB)
          PSPH Antibody in Western Blot (WB)
          PSPH Antibody

          Product Details

          PA5-22003

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Full length human PSPH Recombinant protein.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.1M tris glycine, pH 7, with 10% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11154135

          Product Specific Information

          Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.

          Predicted reactivity: Mouse (93%), Rat (94%), Pig (96%), Chicken (80%), Sheep (93%), Bovine (94%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; OTTHUMP00000025059; Phosphoserine phosphatase; PSP; PSPase

          View more View less

          Gene Aliases: PSP; PSPH; PSPHD

          View more View less

          UniProt ID: (Human) P78330

          View more View less

          Entrez Gene ID: (Human) 5723

          View more View less

          Function(s)
          magnesium ion binding phosphoserine phosphatase activity calcium ion binding protein homodimerization activity
          Process(es)
          L-serine metabolic process L-serine biosynthetic process response to mechanical stimulus dephosphorylation response to nutrient levels response to testosterone
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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