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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PTEN Antibodies

          Invitrogen

          PTEN Monoclonal Antibody (2F4C9)

          Advanced Verification
          4 Published Figures
          5 References
          View all (23) PTEN antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite PTEN Monoclonal Antibody (2F4C9)

          • Antibody Testing Data (6)
          • Published Figures (4)
          • Advanced Verification (1)
          PTEN Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          PTEN Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

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          PTEN Antibody (32-5800) in ICC/IF

          Immunofluorescent analysis of PTEN was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PTEN Mouse Monoclonal Antibody (Product # 32-5800) at 0.5 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at r... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PTEN Antibody in Immunocytochemistry (ICC/IF)
          PTEN Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PTEN Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PTEN Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PTEN Antibody in Western Blot (WB)
          PTEN Antibody in Western Blot (WB)
          PTEN Antibody in Immunocytochemistry (ICC/IF)
          PTEN Antibody in Immunohistochemistry (IHC)
          PTEN Antibody in Western Blot (WB)
          PTEN Antibody in Western Blot (WB)
          PTEN Antibody

          Product Details

          32-5800

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-3 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          0.5 µg/mL
          View 1 publication 1 publication

          ELISA (ELISA)

          0.1-1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          2F4C9

          Immunogen

          Recombinant protein derived from the PTEN protein.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533087

          Product Specific Information

          32-5800 reacts specifically with the human PTEN protein. Reactivity was confirmed with cell lines which contain endogenous PTEN (NIH3T3, K652, and DU145 prostate cancer line) as well as transfected cell lines (Rat1 and 293T cells). This antibody detects the ~55 kDa PTEN protein and was successfully used in western blotting analysis of PTEN in breast cancer cell lines. No cross-reactivity to any other proteins has been detected.

          Target Information

          PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The PTEN gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. PTEN plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Also, PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form of PTEN induces less tumor suppressive ability.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Inositol polyphosphate 3-phosphatase; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; phosphatase and tensin-like protein; phosphatidylinositol 3; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol-3; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; pten tumor suppressor

          View more View less

          Gene Aliases: 10q23del; 2310035O07Rik; A130070J02Rik; AI463227; B430203M17Rik; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN; PTEN1; TEP1

          View more View less

          UniProt ID: (Human) P60484, (Mouse) O08586

          View more View less

          Entrez Gene ID: (Human) 5728, (Mouse) 19211

          View more View less

          Function(s)
          magnesium ion binding phosphatidylinositol-3-phosphatase activity phosphoprotein phosphatase activity protein serine/threonine phosphatase activity protein tyrosine phosphatase activity platelet-derived growth factor receptor binding protein binding protein tyrosine/serine/threonine phosphatase activity lipid binding anaphase-promoting complex binding phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity enzyme binding protein kinase binding PDZ domain binding identical protein binding inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity hydrolase activity phosphatase activity
          Process(es)
          regulation of cyclin-dependent protein serine/threonine kinase activity angiogenesis negative regulation of protein phosphorylation regulation of B cell apoptotic process protein dephosphorylation phosphatidylinositol biosynthetic process apoptotic process neuron-neuron synaptic transmission synapse assembly central nervous system development heart development aging response to nutrient learning or memory memory locomotory behavior cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation response to glucose response to zinc ion regulation of neuron projection development negative regulation of phosphatidylinositol 3-kinase signaling cell migration dentate gyrus development central nervous system neuron axonogenesis negative regulation of cell migration adult behavior negative regulation of myelination regulation of protein stability negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle regulation of synaptic transmission, GABAergic central nervous system myelin maintenance response to estradiol regulation of cellular component size regulation of myeloid cell apoptotic process response to ATP multicellular organismal response to stress social behavior peptidyl-tyrosine dephosphorylation response to drug maternal behavior negative regulation of apoptotic process protein kinase B signaling endothelial cell migration inositol phosphate metabolic process response to ethanol locomotor rhythm negative regulation of cell size negative regulation of organ growth response to arsenic-containing substance inositol phosphate dephosphorylation phosphatidylinositol dephosphorylation platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling regulation of axon regeneration cardiac muscle tissue development forebrain morphogenesis brain morphogenesis negative regulation of epithelial cell proliferation negative regulation of phagocytosis negative regulation of axonogenesis protein stabilization T cell receptor signaling pathway positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of focal adhesion assembly negative regulation of protein kinase B signaling rhythmic synaptic transmission canonical Wnt signaling pathway synapse maturation prepulse inhibition male mating behavior long-term synaptic potentiation long term synaptic depression prostate gland growth dendritic spine morphogenesis negative regulation of dendritic spine morphogenesis negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to hypoxia negative regulation of ribosome biogenesis negative regulation of cell aging negative regulation of excitatory postsynaptic potential presynaptic membrane assembly postsynaptic density assembly positive regulation of TRAIL-activated apoptotic signaling pathway positive regulation of ubiquitin protein ligase activity positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of G1/S transition of mitotic cell cycle positive regulation of excitatory postsynaptic potential negative regulation of synaptic vesicle clustering lipid metabolic process nervous system development dephosphorylation neuron projection development cellular response to decreased oxygen levels positive regulation of apoptotic process regulation of cell cycle positive regulation of apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.
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