Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
PTPN22 Polyclonal Antibody
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 2
PTPN22 Antibody (PA5-142125) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of PTPN22 in U2OS cells using PTPN22 Polyclonal Antibody (Product # PA5-142125). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-142125
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence CPPNKPAESVQSNNS.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932783
Product Specific Information
Additional Information: This antibody is specific for human LYP1 and will not cross-react with LYP2.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.
Target Information
The protein tyrosine phosphatase PTPN22 (PTP22, LYP, PEP, formerly PTPN8) is a genetic variant that confers risk of developing diverse human autoimmune diseases such as type 1 diabetes and rheumatoid arthritis. The minor allele of a missense SNP in PTPN22 encodes a hematopoietic-specific protein tyrosine phosphatase also known as "Lyp." The risk allele is present in about 17% of Caucasian individuals from the general population and in approximately 28% of Caucasian individuals with rheumatoid arthritis; it is thought to disrupt the P1 proline-rich motif that is important for interaction with the Src homology-3 (SH3) domain of CSK (cytoplasmic tyrosine kinase), potentially altering the normal functions of these proteins as negative regulators of T cell activation. The interaction between CSK and PTPN22 is highly specific and it is speculated that PTPN22 may be an effector and/or regulator of CSK in T cells and other hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; lymphoid-specific protein tyrosine phosphatase; LyP; PEP; PEST-domain phosphatase; protein tyrosine phosphatase, non-receptor type 22 (lymphoid); protein tyrosine phosphatase, non-receptor type 8; tyrosine-phosphatase; Tyrosine-protein phosphatase non-receptor type 22
Gene Aliases: LYP; LYP1; LYP2; PEP; PTPN22; PTPN8
UniProt ID: (Human) Q9Y2R2
Entrez Gene ID: (Human) 26191
protein dephosphorylation
autophagy
negative regulation of autophagy
positive regulation of gene expression
negative regulation of gene expression
T cell differentiation
lipopolysaccharide-mediated signaling pathway
positive regulation of type I interferon production
response to lipopolysaccharide
negative regulation of tumor necrosis factor production
regulation of natural killer cell proliferation
positive regulation of toll-like receptor 3 signaling pathway
positive regulation of toll-like receptor 4 signaling pathway
peptidyl-tyrosine dephosphorylation
phosphoanandamide dephosphorylation
negative regulation of JUN kinase activity
regulation of innate immune response
regulation of B cell receptor signaling pathway
negative regulation of T cell receptor signaling pathway
negative regulation of T cell activation
positive regulation of ERK1 and ERK2 cascade
negative regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway
cellular response to muramyl dipeptide
negative regulation of interleukin-6 secretion
regulation of NIK/NF-kappaB signaling
positive regulation of protein K63-linked ubiquitination
positive regulation of interferon-gamma secretion
negative regulation of p38MAPK cascade
negative regulation of interleukin-8 secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support