Proteintech

PTPN23 Polyclonal Antibody

PTPN23 Antibody in Immunocytochemistry (ICC/IF)

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PTPN23 Antibody (10472-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PTPN23 antibody (Product # 10472-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PTPN23 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PTPN23 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10472-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:50-1:500

View 6 publications 6 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PTPN23 Fusion Protein Ag0765 (420-619 aa encoded by BC004881)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.38 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: QEKLHLRFC YEAVVRHVEQ VLQRHGVPPP CKPLASASIS QKNHLPQDSQ DLVLGGDVPI SSIQATIAKL SIRPPGGLES PVASLPGPAE PPGLPPASLP ESTPIPSSSP PPLSSPLPEA PQPKEEPPVP EAPSSGPPSS SLELLASLTP EAFSLDSSLR GKQRMSKHNF LQAHNGQGLR ATRPSDDPLS LLDPLWTLNK T (420-619 aa encoded by BC004881)

Target Information

Plays a role in sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs) via its interaction with the ESCRT-I complex (endosomal sorting complex required for transport I), and possibly also other ESCRT complexes. May act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation.

Journal of cell science

Parkinson G,Roboti P,Zhang L,Taylor S,Woodman P

Mon Nov 01 00:00:00 UTC 2021

His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation.

Journal of cell science

Parkinson G,Roboti P,Zhang L,Taylor S,Woodman P

Mon Nov 01 00:00:00 UTC 2021

Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.

iScience

Kazan JM,Desrochers G,Martin CE,Jeong H,Kharitidi D,Apaja PM,Roldan A,St Denis N,Gingras AC,Lukacs GL,Pause A

Fri Nov 19 00:00:00 UTC 2021

A combined EM and proteomic analysis places HIV-1 Vpu at the crossroads of retromer and ESCRT complexes: PTPN23 is a Vpu-cofactor.

PLoS pathogens

Stoneham CA,Langer S,De Jesus PD,Wozniak JM,Lapek J,Deerinck T,Thor A,Pache L,Chanda SK,Gonzalez DJ,Ellisman M,Guatelli J

Mon Nov 01 00:00:00 UTC 2021

A combined EM and proteomic analysis places HIV-1 Vpu at the crossroads of retromer and ESCRT complexes: PTPN23 is a Vpu-cofactor.

PLoS pathogens

Stoneham CA,Langer S,De Jesus PD,Wozniak JM,Lapek J,Deerinck T,Thor A,Pache L,Chanda SK,Gonzalez DJ,Ellisman M,Guatelli J

Mon Nov 01 00:00:00 UTC 2021

ESCRT-mediated lysosome repair precedes lysophagy and promotes cell survival.

The EMBO journal

Radulovic M,Schink KO,Wenzel EM,Nähse V,Bongiovanni A,Lafont F,Stenmark H

Fri Nov 02 00:00:00 UTC 2018

Concerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation.

Nature communications

Wenzel EM,Schultz SW,Schink KO,Pedersen NM,Nähse V,Carlson A,Brech A,Stenmark H,Raiborg C

Thu Jul 26 00:00:00 UTC 2018

Concerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation.

Nature communications

Wenzel EM,Schultz SW,Schink KO,Pedersen NM,Nähse V,Carlson A,Brech A,Stenmark H,Raiborg C

Thu Jul 26 00:00:00 UTC 2018

Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor-dependent manner.

The Journal of biological chemistry

Gosney JA,Wilkey DW,Merchant ML,Ceresa BP

Fri Apr 20 00:00:00 UTC 2018

Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor-dependent manner.

The Journal of biological chemistry

Gosney JA,Wilkey DW,Merchant ML,Ceresa BP

Fri Apr 20 00:00:00 UTC 2018

Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor-dependent manner.

The Journal of biological chemistry

Gosney JA,Wilkey DW,Merchant ML,Ceresa BP

Fri Apr 20 00:00:00 UTC 2018

Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor-dependent manner.

The Journal of biological chemistry

Gosney JA,Wilkey DW,Merchant ML,Ceresa BP

Fri Apr 20 00:00:00 UTC 2018

Suppression of protein tyrosine phosphatase N23 predisposes to breast tumorigenesis via activation of FYN kinase.

Genes & development

Zhang S,Fan G,Hao Y,Hammell M,Wilkinson JE,Tonks NK

Sun Oct 01 00:00:00 UTC 2017

Suppression of protein tyrosine phosphatase N23 predisposes to breast tumorigenesis via activation of FYN kinase.

Genes & development

Zhang S,Fan G,Hao Y,Hammell M,Wilkinson JE,Tonks NK

Sun Oct 01 00:00:00 UTC 2017

Suppression of protein tyrosine phosphatase N23 predisposes to breast tumorigenesis via activation of FYN kinase.

Genes & development

Zhang S,Fan G,Hao Y,Hammell M,Wilkinson JE,Tonks NK

Sun Oct 01 00:00:00 UTC 2017

Suppression of protein tyrosine phosphatase N23 predisposes to breast tumorigenesis via activation of FYN kinase.

Genes & development

Zhang S,Fan G,Hao Y,Hammell M,Wilkinson JE,Tonks NK

Sun Oct 01 00:00:00 UTC 2017

Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.

Structure (London, England : 1993)

Gahloth D,Levy C,Walker L,Wunderley L,Mould AP,Taylor S,Woodman P,Tabernero L

Wed Jul 05 00:00:00 UTC 2017

Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.

Structure (London, England : 1993)

Gahloth D,Levy C,Walker L,Wunderley L,Mould AP,Taylor S,Woodman P,Tabernero L

Wed Jul 05 00:00:00 UTC 2017

Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9.

Cell reports

Goodwin JM,Dowdle WE,DeJesus R,Wang Z,Bergman P,Kobylarz M,Lindeman A,Xavier RJ,McAllister G,Nyfeler B,Hoffman G,Murphy LO

Tue Sep 05 00:00:00 UTC 2017

Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9.

Cell reports

Goodwin JM,Dowdle WE,DeJesus R,Wang Z,Bergman P,Kobylarz M,Lindeman A,Xavier RJ,McAllister G,Nyfeler B,Hoffman G,Murphy LO

Tue Sep 05 00:00:00 UTC 2017

Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB.

Current biology : CB

Ali N,Zhang L,Taylor S,Mironov A,Urbé S,Woodman P

Mon Mar 18 00:00:00 UTC 2013

Tumor-suppressive function of protein-tyrosine phosphatase non-receptor type 23 in testicular germ cell tumors is lost upon overexpression of miR142-3p microRNA.

The Journal of biological chemistry

Tanaka K,Kondo K,Kitajima K,Muraoka M,Nozawa A,Hara T

Fri Aug 16 00:00:00 UTC 2013

Tumor-suppressive function of protein-tyrosine phosphatase non-receptor type 23 in testicular germ cell tumors is lost upon overexpression of miR142-3p microRNA.

The Journal of biological chemistry

Tanaka K,Kondo K,Kitajima K,Muraoka M,Nozawa A,Hara T

Fri Aug 16 00:00:00 UTC 2013

Tumor-suppressive function of protein-tyrosine phosphatase non-receptor type 23 in testicular germ cell tumors is lost upon overexpression of miR142-3p microRNA.

The Journal of biological chemistry

Tanaka K,Kondo K,Kitajima K,Muraoka M,Nozawa A,Hara T

Fri Aug 16 00:00:00 UTC 2013

Tumor-suppressive function of protein-tyrosine phosphatase non-receptor type 23 in testicular germ cell tumors is lost upon overexpression of miR142-3p microRNA.

The Journal of biological chemistry

Tanaka K,Kondo K,Kitajima K,Muraoka M,Nozawa A,Hara T

Fri Aug 16 00:00:00 UTC 2013

The Bro1-domain-containing protein Myopic/HDPTP coordinates with Rab4 to regulate cell adhesion and migration.

Journal of cell science

Chen DY,Li MY,Wu SY,Lin YL,Tsai SP,Lai PL,Lin YT,Kuo JC,Meng TC,Chen GC

Mon Oct 15 00:00:00 UTC 2012

The Bro1-domain-containing protein Myopic/HDPTP coordinates with Rab4 to regulate cell adhesion and migration.

Journal of cell science

Chen DY,Li MY,Wu SY,Lin YL,Tsai SP,Lai PL,Lin YT,Kuo JC,Meng TC,Chen GC

Mon Oct 15 00:00:00 UTC 2012

UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.

Current biology : CB

Stefani F,Zhang L,Taylor S,Donovan J,Rollinson S,Doyotte A,Brownhill K,Bennion J,Pickering-Brown S,Woodman P

Tue Jul 26 00:00:00 UTC 2011

UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.

Current biology : CB

Stefani F,Zhang L,Taylor S,Donovan J,Rollinson S,Doyotte A,Brownhill K,Bennion J,Pickering-Brown S,Woodman P

Tue Jul 26 00:00:00 UTC 2011

UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.

Current biology : CB

Stefani F,Zhang L,Taylor S,Donovan J,Rollinson S,Doyotte A,Brownhill K,Bennion J,Pickering-Brown S,Woodman P

Tue Jul 26 00:00:00 UTC 2011

UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.

Current biology : CB

Stefani F,Zhang L,Taylor S,Donovan J,Rollinson S,Doyotte A,Brownhill K,Bennion J,Pickering-Brown S,Woodman P

Tue Jul 26 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: HD-PTP; His domain-containing protein tyrosine phosphatase; Protein tyrosine phosphatase TD14; PTP-TD14; Tyrosine-protein phosphatase non-receptor type 23

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Gene Aliases: HD-PTP; HDPTP; KIAA1471; PTP-TD14; PTPN23

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UniProt ID: (Human) Q9H3S7

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Entrez Gene ID: (Human) 25930

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Function(s)

protein tyrosine phosphatase activity protein binding protein kinase binding

Process(es)

negative regulation of epithelial cell migration protein transport peptidyl-tyrosine dephosphorylation ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway cilium morphogenesis positive regulation of homophilic cell adhesion positive regulation of adherens junction organization positive regulation of early endosome to late endosome transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PTPN23 Polyclonal Antibody

PTPN23 Antibody (10472-1-AP) in ICC/IF

Product Details

10472-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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