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PTPN3 Antibody (A305-885A-T) in WB
Detection of human PTPN3 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, RKO, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PTPN3 antibody A305-885A-M (lot A305-885A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A305-885A-T
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A region between residue 175 and 225 of human Tyrosine-protein phosphatase non-receptor type 3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7.0 to 8.0
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Wet ice
Product Specific Information
Predicted reactivity by homology: Mouse.
Target Information
Phosphorylation of receptors by protein kinases is a process that can be reversed by a group of enzymes called protein phosphatases. Coordinated control of kinases and phosphatases provides the cell with the capacity to rapidly switch between phosphorylated and dephosphorylated protein states in dynamic response to environmental stimuli. Activation of critical enzymes by kinase phosphorylation alone is not enough to provide adequate regulation - it is the combination with phosphatase dephosphorylation that effectively creates on/off switches to control cellular events. Errors in control, either through kinases or their counterpart phosphatases, can lead to unchecked cell growth attributable to human cancers and developmental disorders. Potential mechanisms to control dephosphorylation include changes in the expression of protein phosphatases, their subcellular localization, phosphorylation of phosphatase catalytic and regulatory subunits and regulation by endogenous phosphatase inhibitors. Most protein phosphatases are not stringently specific for their substrates. Consequently, changes in phosphatase activity may have a broad impact on dephosphorylation and turnover of phosphoproteins that are substrates for different kinases. This may be an important point of control to connect cellular circuitry of interrelated signaling pathways, and to synchronize physiological responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytoskeletal-associated protein tyrosine phosphatase; Protein-tyrosine phosphatase H1; PTP-H1; Tyrosine-protein phosphatase non-receptor type 3
Gene Aliases: PTP-H1; PTPH1; PTPN3
UniProt ID: (Human) P26045
Entrez Gene ID: (Human) 5774
protein dephosphorylation
peptidyl-tyrosine dephosphorylation
negative regulation of mitotic cell cycle
negative regulation of membrane protein ectodomain proteolysis
liver regeneration
regulation of membrane depolarization during action potential
regulation of sodium ion transmembrane transporter activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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