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Invitrogen
PTPRN Polyclonal Antibody
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FIGURE: 1 / 3
PTPRN Antibody (PA5-76932) in ICC/IF
Immunofluorescence analysis of PTPRN was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PTPRN Polyclonal Antibody (Product # PA5-76932) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (... View More
Product Details
PA5-76932
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human PTPRN.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2720659
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
IA-2 (insulinoma-associated protein 2, ICA512, PTPRN) is a member of the transmembrane protein tyrosine phosphatase (PTP) family located in secretory granules of neuroendocrine cells. The IA-2 protein is 979 amino acids in length and consists of a luminal domain, transmembrane domain, and cytoplasmic domain. Although a member of the PTP family, IA-2 lacks phosphatase activity with known substrates due to amino acid substitutions at critical sites in its PTP domain. Two paralog RPTPs, IA-2 and IA-2beta were identified as major autoantigens in type-1 diabetes mellitus. On granule exocytosis, the IA-2 cytoplasmic domain is cleaved and the resulting cytosolic fragment moves into the nucleus where it enhances the levels of phosphorylated STAT5 and STAT3, thereby inducing insulin gene transcription and granule biogenesis. IA-2 signaling enhances pancreatic beta-cell proliferation by regulating cyclins D through STATs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 105 kDa islet cell antigen; BEM-3; Brain-enriched membrane-associated protein tyrosine phosphatase; ICA 512; ICA105; ICA512; islet cell antigen 2; Islet cell antigen 512; Islet cell autoantigen 3; protein tyrosine phosphatase, receptor type, N; protein tyrosine phosphatase, receptor-type, N; protein tyrosine phosphatase-like N; PTP IA-2; PTPLP; R-PTP-N; Receptor-type tyrosine-protein phosphatase-like N
Gene Aliases: IA-2; IA-2/PTP; IA2; ICA3; ICA512; mIA-A; mKIAA4064; PTPRN; R-PTP-N
UniProt ID: (Human) Q16849, (Rat) Q63259
Entrez Gene ID: (Human) 5798, (Rat) 116660, (Mouse) 19275
response to reactive oxygen species
protein dephosphorylation
response to glucose
cytokine-mediated signaling pathway
second-messenger-mediated signaling
insulin secretion
response to insulin
response to estrogen
response to cAMP
dense core granule maturation
luteinization
insulin secretion involved in cellular response to glucose stimulus
dephosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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