Proteintech

PTRF Polyclonal Antibody

PTRF Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

PTRF Antibody (18892-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using PTRF antibody 18892-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

PTRF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PTRF Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

18892-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 24 publications 24 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PTRF Fusion Protein Ag13486 (1-198 aa encoded by BC008849)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEDAAALEL SSDEAVEVEE VIEESRAERI KRSGLRRVDD FKKAFSKEKM EKTKVRTREN LEKTRLKTKE NLEKTRHTLE KRMNKLGTRL VPAERREKLK TSRDKLRKSF TPDHVVYARS KTAVYKVPPF TFHVKKIREG QVEVLKATEM VEVGADDDEG GAERGEAGDL RRGSSPDVHA LLEITEESDA VLVDKSDSD (1-198 aa encoded by BC008849 )

Target Information

The polymerase I and transcript release factor (PTRF) enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts, regulating rRNA transcription by promoting the dissociation of transcription complexes and the re-initiation of polymerase I on nascent rRNA transcripts. PTRF also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins, translocating from caveolae to the cytoplasm after insulin stimulation. PTRF is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Serum amyloid A and interleukin -1β facilitate LDL transcytosis across endothelial cells and atherosclerosis via NF-κB/caveolin-1/cavin-1 pathway.

Atherosclerosis

Jia X,Liu Z,Wang Y,Li G,Bai X

Thu Jun 01 00:00:00 UTC 2023

Serum amyloid A and interleukin -1β facilitate LDL transcytosis across endothelial cells and atherosclerosis via NF-κB/caveolin-1/cavin-1 pathway.

Atherosclerosis

Jia X,Liu Z,Wang Y,Li G,Bai X

Thu Jun 01 00:00:00 UTC 2023

Serum amyloid A and interleukin -1β facilitate LDL transcytosis across endothelial cells and atherosclerosis via NF-κB/caveolin-1/cavin-1 pathway.

Atherosclerosis

Jia X,Liu Z,Wang Y,Li G,Bai X

Thu Jun 01 00:00:00 UTC 2023

Serum amyloid A and interleukin -1β facilitate LDL transcytosis across endothelial cells and atherosclerosis via NF-κB/caveolin-1/cavin-1 pathway.

Atherosclerosis

Jia X,Liu Z,Wang Y,Li G,Bai X

Thu Jun 01 00:00:00 UTC 2023

Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2.

Developmental cell

Wu Y,Lim YW,Stroud DA,Martel N,Hall TE,Lo HP,Ferguson C,Ryan MT,McMahon KA,Parton RG

Mon Mar 13 00:00:00 UTC 2023

Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2.

Developmental cell

Wu Y,Lim YW,Stroud DA,Martel N,Hall TE,Lo HP,Ferguson C,Ryan MT,McMahon KA,Parton RG

Mon Mar 13 00:00:00 UTC 2023

Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2.

Developmental cell

Wu Y,Lim YW,Stroud DA,Martel N,Hall TE,Lo HP,Ferguson C,Ryan MT,McMahon KA,Parton RG

Mon Mar 13 00:00:00 UTC 2023

DHCR24 Knockdown Induces Tau Hyperphosphorylation at Thr181, Ser199, Ser262, and Ser396 Sites via Activation of the Lipid Raft-Dependent Ras/MEK/ERK Signaling Pathway in C8D1A Astrocytes.

Molecular neurobiology

Mai M,Guo X,Huang Y,Zhang W,Xu Y,Zhang Y,Bai X,Wu J,Zu H

Thu Sep 01 00:00:00 UTC 2022

PTRF/Cavin-1 enhances chemo-resistance and promotes temozolomide efflux through extracellular vesicles in glioblastoma.

Theranostics

Yang E,Wang L,Jin W,Liu X,Wang Q,Wu Y,Tan Y,Wang Y,Cui X,Zhao J,Tong F,Hong B,Xiao M,Liu X,Fang C,Kang C

Thu Jun 09 00:00:00 UTC 2022

UBE2O ubiquitinates PTRF/CAVIN1 and inhibits the secretion of exosome-related PTRF/CAVIN1.

Cell communication and signaling : CCS

Cen X,Chen Q,Wang B,Xu H,Wang X,Ling Y,Zhang X,Qin D

Mon Nov 28 00:00:00 UTC 2022

UBE2O ubiquitinates PTRF/CAVIN1 and inhibits the secretion of exosome-related PTRF/CAVIN1.

Cell communication and signaling : CCS

Cen X,Chen Q,Wang B,Xu H,Wang X,Ling Y,Zhang X,Qin D

Mon Nov 28 00:00:00 UTC 2022

A Novel High-Throughput Screening Method for a Human Multicentric Osteosarcoma-Specific Antibody and Biomarker Using a Phage Display-Derived Monoclonal Antibody.

Cancers

Hayashi T,Yamamoto N,Kurosawa G,Tajima K,Kondo M,Hiramatsu N,Kato Y,Tanaka M,Yamaguchi H,Kurosawa Y,Yamada H,Fujita N

Sat Nov 26 00:00:00 UTC 2022

Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.

Science advances

Andrade V,Bai J,Gupta-Rossi N,Jimenez AJ,Delevoye C,Lamaze C,Echard A

Fri Apr 15 00:00:00 UTC 2022

Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.

Science advances

Andrade V,Bai J,Gupta-Rossi N,Jimenez AJ,Delevoye C,Lamaze C,Echard A

Fri Apr 15 00:00:00 UTC 2022

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

ACS nano

Liao Z,Liu H,Ma L,Lei J,Tong B,Li G,Ke W,Wang K,Feng X,Hua W,Li S,Yang C

Tue Sep 28 00:00:00 UTC 2021

PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma.

Frontiers in immunology

Yi K,Cui X,Liu X,Wang Y,Zhao J,Yang S,Xu C,Yang E,Xiao M,Hong B,Fang C,Kang C,Tan Y,Wang Q

Wed Feb 23 00:00:00 UTC 2022

EphB1 interaction with caveolin-1 in endothelial cells modulates caveolae biogenesis.

Molecular biology of the cell

Tiruppathi C,Regmi SC,Wang DM,Mo GCH,Toth PT,Vogel SM,Stan RV,Henkemeyer M,Minshall RD,Rehman J,Malik AB

Fri May 15 00:00:00 UTC 2020

Synaptotagmin-11 regulates the functions of caveolae and responds to mechanical stimuli in astrocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Yan S,Wang Y,Zhang Y,Wang L,Zhao X,Du C,Gao P,Yan F,Liu F,Gong X,Guan Y,Cui X,Wang X,Xi Zhang C

Sat Feb 01 00:00:00 UTC 2020

Salidroside-Mediated Autophagic Targeting of Active Src and Caveolin-1 Suppresses Low-Density Lipoprotein Transcytosis across Endothelial Cells.

Oxidative medicine and cellular longevity

Bai X,Jia X,Lu Y,Zhu L,Zhao Y,Cheng W,Shu M,Jin S

Fri May 14 00:00:00 UTC 2021

CAV1-CAVIN1-LC3B-mediated autophagy regulates high glucose-stimulated LDL transcytosis.

Autophagy

Bai X,Yang X,Jia X,Rong Y,Chen L,Zeng T,Deng X,Li W,Wu G,Wang L,Li Y,Zhang J,Xiong Z,Xiong L,Wang Y,Zhu L,Zhao Y,Jin S

Mon Jun 01 00:00:00 UTC 2020

CAV1-CAVIN1-LC3B-mediated autophagy regulates high glucose-stimulated LDL transcytosis.

Autophagy

Bai X,Yang X,Jia X,Rong Y,Chen L,Zeng T,Deng X,Li W,Wu G,Wang L,Li Y,Zhang J,Xiong Z,Xiong L,Wang Y,Zhu L,Zhao Y,Jin S

Mon Jun 01 00:00:00 UTC 2020

A role for caveola-forming proteins caveolin-1 and CAVIN1 in the pro-invasive response of glioblastoma to osmotic and hydrostatic pressure.

Journal of cellular and molecular medicine

Pu W,Qiu J,Nassar ZD,Shaw PN,McMahon KA,Ferguson C,Parton RG,Riggins GJ,Harris JM,Parat MO

Sun Mar 01 00:00:00 UTC 2020

Homotrimer cavin1 interacts with caveolin1 to facilitate tumor growth and activate microglia through extracellular vesicles in glioma.

Theranostics

Wang L,Yang C,Wang Q,Liu Q,Wang Y,Zhou J,Li Y,Tan Y,Kang C

Tue May 18 00:00:00 UTC 2021

Homotrimer cavin1 interacts with caveolin1 to facilitate tumor growth and activate microglia through extracellular vesicles in glioma.

Theranostics

Wang L,Yang C,Wang Q,Liu Q,Wang Y,Zhou J,Li Y,Tan Y,Kang C

Tue May 18 00:00:00 UTC 2021

Correlation of the invasive potential of glioblastoma and expression of caveola-forming proteins caveolin-1 and CAVIN1.

Journal of neuro-oncology

Pu W,Nassar ZD,Khabbazi S,Xie N,McMahon KA,Parton RG,Riggins GJ,Harris JM,Parat MO

Sat Jun 01 00:00:00 UTC 2019

Inducible Fgf13 ablation enhances caveolae-mediated cardioprotection during cardiac pressure overload.

Proceedings of the National Academy of Sciences of the United States of America

Wei EQ,Sinden DS,Mao L,Zhang H,Wang C,Pitt GS

Tue May 16 00:00:00 UTC 2017

Inducible Fgf13 ablation enhances caveolae-mediated cardioprotection during cardiac pressure overload.

Proceedings of the National Academy of Sciences of the United States of America

Wei EQ,Sinden DS,Mao L,Zhang H,Wang C,Pitt GS

Tue May 16 00:00:00 UTC 2017

Reactive oxygen species mediate angiotensin II-induced transcytosis of low-density lipoprotein across endothelial cells.

International journal of molecular medicine

Bian F,Cui J,Zheng T,Jin S

Wed Mar 01 00:00:00 UTC 2017

Reactive oxygen species mediate angiotensin II-induced transcytosis of low-density lipoprotein across endothelial cells.

International journal of molecular medicine

Bian F,Cui J,Zheng T,Jin S

Wed Mar 01 00:00:00 UTC 2017

Acetylation of Cavin-1 Promotes Lipolysis in White Adipose Tissue.

Molecular and cellular biology

Zhou SR,Guo L,Wang X,Liu Y,Peng WQ,Liu Y,Wei XB,Dou X,Ding M,Lei QY,Qian SW,Li X,Tang QQ

Tue Aug 15 00:00:00 UTC 2017

Acetylation of Cavin-1 Promotes Lipolysis in White Adipose Tissue.

Molecular and cellular biology

Zhou SR,Guo L,Wang X,Liu Y,Peng WQ,Liu Y,Wei XB,Dou X,Ding M,Lei QY,Qian SW,Li X,Tang QQ

Tue Aug 15 00:00:00 UTC 2017

Acetylation of Cavin-1 Promotes Lipolysis in White Adipose Tissue.

Molecular and cellular biology

Zhou SR,Guo L,Wang X,Liu Y,Peng WQ,Liu Y,Wei XB,Dou X,Ding M,Lei QY,Qian SW,Li X,Tang QQ

Tue Aug 15 00:00:00 UTC 2017

Acetylation of Cavin-1 Promotes Lipolysis in White Adipose Tissue.

Molecular and cellular biology

Zhou SR,Guo L,Wang X,Liu Y,Peng WQ,Liu Y,Wei XB,Dou X,Ding M,Lei QY,Qian SW,Li X,Tang QQ

Tue Aug 15 00:00:00 UTC 2017

Cavin-1 regulates caveolae-mediated LDL transcytosis: crosstalk in an AMPK/eNOS/ NF-κB/Sp1 loop.

Oncotarget

Bai XL,Yang XY,Li JY,Jia X,Xiong ZF,Wang YM,Jin S

Tue Nov 28 00:00:00 UTC 2017

Cavin-1 regulates caveolae-mediated LDL transcytosis: crosstalk in an AMPK/eNOS/ NF-κB/Sp1 loop.

Oncotarget

Bai XL,Yang XY,Li JY,Jia X,Xiong ZF,Wang YM,Jin S

Tue Nov 28 00:00:00 UTC 2017

Deciphering caveolar functions by syndapin III KO-mediated impairment of caveolar invagination.

eLife

Seemann E,Sun M,Krueger S,Tröger J,Hou W,Haag N,Schüler S,Westermann M,Huebner CA,Romeike B,Kessels MM,Qualmann B

Tue Dec 05 00:00:00 UTC 2017

Deciphering caveolar functions by syndapin III KO-mediated impairment of caveolar invagination.

eLife

Seemann E,Sun M,Krueger S,Tröger J,Hou W,Haag N,Schüler S,Westermann M,Huebner CA,Romeike B,Kessels MM,Qualmann B

Tue Dec 05 00:00:00 UTC 2017

Deciphering caveolar functions by syndapin III KO-mediated impairment of caveolar invagination.

eLife

Seemann E,Sun M,Krueger S,Tröger J,Hou W,Haag N,Schüler S,Westermann M,Huebner CA,Romeike B,Kessels MM,Qualmann B

Tue Dec 05 00:00:00 UTC 2017

Deciphering caveolar functions by syndapin III KO-mediated impairment of caveolar invagination.

eLife

Seemann E,Sun M,Krueger S,Tröger J,Hou W,Haag N,Schüler S,Westermann M,Huebner CA,Romeike B,Kessels MM,Qualmann B

Tue Dec 05 00:00:00 UTC 2017

Src-dependent phosphorylation of caveolin-1 Tyr-14 promotes swelling and release of caveolae.

Molecular biology of the cell

Zimnicka AM,Husain YS,Shajahan AN,Sverdlov M,Chaga O,Chen Z,Toth PT,Klomp J,Karginov AV,Tiruppathi C,Malik AB,Minshall RD

Fri Jul 01 00:00:00 UTC 2016

Hypoxia inhibits Cavin-1 and Cavin-2 expression and down-regulates caveolae in adipocytes.

Endocrinology

Regazzetti C,Dumas K,Lacas-Gervais S,Pastor F,Peraldi P,Bonnafous S,Dugail I,Le Lay S,Valet P,Le Marchand-Brustel Y,Tran A,Gual P,Tanti JF,Cormont M,Giorgetti-Peraldi S

Sun Mar 01 00:00:00 UTC 2015

Hypoxia inhibits Cavin-1 and Cavin-2 expression and down-regulates caveolae in adipocytes.

Endocrinology

Regazzetti C,Dumas K,Lacas-Gervais S,Pastor F,Peraldi P,Bonnafous S,Dugail I,Le Lay S,Valet P,Le Marchand-Brustel Y,Tran A,Gual P,Tanti JF,Cormont M,Giorgetti-Peraldi S

Sun Mar 01 00:00:00 UTC 2015

Non-caveolar caveolin-1 expression in prostate cancer cells promotes lymphangiogenesis.

Oncoscience

Nassar ZD,Hill MM,Parton RG,Francois M,Parat MO

Wed Sep 02 00:00:00 UTC 2015

Non-caveolar caveolin-1 expression in prostate cancer cells promotes lymphangiogenesis.

Oncoscience

Nassar ZD,Hill MM,Parton RG,Francois M,Parat MO

Wed Sep 02 00:00:00 UTC 2015

Cavin-1/PTRF alters prostate cancer cell-derived extracellular vesicle content and internalization to attenuate extracellular vesicle-mediated osteoclastogenesis and osteoblast proliferation.

Journal of extracellular vesicles

Inder KL,Ruelcke JE,Petelin L,Moon H,Choi E,Rae J,Blumenthal A,Hutmacher D,Saunders NA,Stow JL,Parton RG,Hill MM

Mon Jul 14 00:00:00 UTC 2014

Cavin-1/PTRF alters prostate cancer cell-derived extracellular vesicle content and internalization to attenuate extracellular vesicle-mediated osteoclastogenesis and osteoblast proliferation.

Journal of extracellular vesicles

Inder KL,Ruelcke JE,Petelin L,Moon H,Choi E,Rae J,Blumenthal A,Hutmacher D,Saunders NA,Stow JL,Parton RG,Hill MM

Mon Jul 14 00:00:00 UTC 2014

Mitsugumin 53 protects the kidney from severe burn injury in mice.

Burns & trauma

Wu Y,Huang J,Liu D,Tan J,Peng Y,Yang J,Cui Y,He W,Luo G,Wu J

Tue Aug 30 00:00:00 UTC 2016

Mitsugumin 53 protects the kidney from severe burn injury in mice.

Burns & trauma

Wu Y,Huang J,Liu D,Tan J,Peng Y,Yang J,Cui Y,He W,Luo G,Wu J

Tue Aug 30 00:00:00 UTC 2016

PTRF/Cavin-1 decreases prostate cancer angiogenesis and lymphangiogenesis.

Oncotarget

Nassar ZD,Moon H,Duong T,Neo L,Hill MM,Francois M,Parton RG,Parat MO

Tue Oct 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Cav-p60; Caveolae-associated protein 1; cavin; Cavin-1; FLJ90031; Polymerase I and transcript release factor; PTRF; ptrf {ECO:0000312|RGD:1307685}; RNA polymerase I and transcript release factor; TTF-I interacting peptide 12

View more View less

Gene Aliases: 2310075E07Rik; AW546441; Cav-p60; CAVIN; cavin-1; CAVIN1; CGL4; FKSG13; PTRF

View more View less

UniProt ID: (Human) Q6NZI2, (Mouse) O54724

View more View less

Entrez Gene ID: (Human) 284119, (Rat) 287710, (Mouse) 19285

View more View less

Function(s)

protein binding rRNA primary transcript binding poly(A) RNA binding RNA binding rRNA binding

Process(es)

regulation of transcription, DNA-templated transcription initiation from RNA polymerase I promoter termination of RNA polymerase I transcription transcription, DNA-templated DNA-templated transcription, termination

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

PTRF Polyclonal Antibody

PTRF Antibody (18892-1-AP) in ICC/IF

Product Details

18892-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!