NeoBiotechnologies

PU.1 (SPI-1) Monoclonal Antibody (PU1, 2146)

View all (33) PU.1 antibodies

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

6688-MSM2-P1

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

1-2 µg/mL
-

Flow Cytometry (Flow)

1-2 µg/10^6 cells
-

Peptide Array (Array)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

PU1, 2146

Immunogen

Recombinant fragment (around aa 16-170) of human PU.1 protein
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: 31 kDa transforming protein; 31 kDa-transforming protein; 31kDa Transforming Protein; hCG_25181; hematopoietic transcription factor PU.1; SPIA; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1; Transcription Factor spi1

View more View less

Gene Aliases: OF; PU.1; SFPI1; SPI-1; SPI-A; SPI1

View more View less

UniProt ID: (Human) P17947

View more View less

Entrez Gene ID: (Human) 6688

View more View less

Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding RNA binding protein binding NFAT protein binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter lymphoid progenitor cell differentiation transcription from RNA polymerase II promoter lymphocyte differentiation erythrocyte differentiation macrophage differentiation granulocyte differentiation somatic stem cell population maintenance myeloid dendritic cell differentiation histone H3 acetylation hypermethylation of CpG island negative regulation of MHC class II biosynthetic process regulation of erythrocyte differentiation negative regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter anatomical structure regression cellular response to ethanol negative regulation of histone H4 acetylation apoptotic process involved in patterning of blood vessels positive regulation of pri-miRNA transcription from RNA polymerase II promoter
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support