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Invitrogen
PUM1 Recombinant Rabbit Monoclonal Antibody (JG37-75)
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FIGURE: 1 / 8
PUM1 Antibody (MA5-41264) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of PUM1 in SiHa cells (green) using PUM1 Recombinant Monoclonal Antibody (Product # MA5-41264). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. The nuclear counter stain is DAPI (blue).
Product Details
MA5-41264
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JG37-75
Immunogen
Recombinant protein within Human Pumilio 1 aa 79-223
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899017
Product Specific Information
This product is predicted to work in Immunoprecipitation at a dilution of 1:10-1:50.
Target Information
PUM1 (pumilio homolog 1) is a sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. It is a member of the PUF family comprised of evolutionarily conserved RNA-binding proteins. PUM1 binds to an RNA consensus sequence, the Pumilio Response Element (PRE), and 5'-UGUANAUA-3' that is related to the Nanos Response Element (NRE). PUM1 mediates post-transcriptonal repression of transcripts via different mechanisms - acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Mutations to the gene can result in spinocerebellar ataxia 47.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HsPUM; pumilio 1; Pumilio homolog 1; pumilio RNA-binding family member 1; pumilio-1
Gene Aliases: AA517475; HSPUM; KIAA0099; mKIAA0099; PUM1; PUMH; PUMH1; PUML1; Pumm
UniProt ID: (Human) Q14671, (Mouse) Q80U78
Entrez Gene ID: (Human) 9698, (Mouse) 80912
spermatogenesis
adult locomotory behavior
posttranscriptional regulation of gene expression
posttranscriptional gene silencing
regulation of mRNA stability
stem cell differentiation
regulation of cell cycle
regulation of chromosome segregation
regulation of gene silencing by miRNA
mRNA destabilization
positive regulation of RIG-I signaling pathway
positive regulation of gene silencing by miRNA
regulation of translation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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