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PUS10 Antibody (PA5-90861) in WB
Western blot analysis of PUS10 in extracts of various cell lines. Samples were incubated with PUS10 Polyclonal antibody (Product # PA5-90861) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-90861
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_6533102)
Conjugate
Form
Liquid
Concentration
0.9 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806265
Product Specific Information
Immunogen sequence: VAGRYNKYSR NLPQTPWIID GERKLESSVE ELISDHLLAV FKAESFNFSS SGREDVDVRT LGNGRPFAIE LVNPHRVHFT SQEIKELQQK INNSSNKIQV RDLQLVTREA IGHMKEGEEE KTKTYSALIW TNKAIQKKDI EFLNDIKDLK IDQKTPLRVL HRRPLAVRAR VIHFMETQYV DEHHFRLHLK TQAGTYIKEF VHGDFGRTKP NIGSLMNVTA DILELDVESV DVDWPPALDD; Positive Samples: Mouse thymus, Mouse spleen, Mouse testis, Rat thymus
Target Information
Pseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: coiled-coil domain containing 139; Coiled-coil domain-containing protein 139; Psi55 synthase; putative tRNA pseudouridine synthase Pus10; tRNA pseudouridine 55 synthase; tRNA pseudouridine synthase Pus10; tRNA pseudouridylate synthase; tRNA-uridine isomerase
Gene Aliases: 2810013G11Rik; 4933435A13Rik; AU014648; C77560; Ccdc139; Pus10; RGD1306402
UniProt ID: (Mouse) Q9D3U0
Entrez Gene ID: (Rat) 305583, (Mouse) 74467
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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