Invitrogen

PYCARD Recombinant Rabbit Monoclonal Antibody (SN07-10)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-32312

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:400-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

Flow Cytometry (Flow)

1:50-1:100
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SN07-10

Immunogen

Synthetic peptide within Human TMS1 aa 121-170
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809594

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions. Required for recruitment of caspase-1 to inflammasomes containing certain pattern recognition receptors, such as NLRP2, NLRP3, AIM2 and probably IFI16. In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in DDX58-triggered proinflammatory responses and inflammasome activation. Isoform 2 may have a regulating effect on the function as inflammasome adapter. Isoform 3 seems to inhibit inflammasome-mediated maturation of interleukin-1 beta. In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8. In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form. Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways. For regulation of NF-kappa-B activating and inhibiting functions have been reported. Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK. Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Apoptosis-associated speck-like protein containing a CARD; Caspase recruitment domain-containing protein 5; hASC; MGC10332; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1

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Gene Aliases: ASC; CARD5; PYCARD; TMS; TMS-1; TMS1

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UniProt ID: (Human) Q9ULZ3

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Entrez Gene ID: (Human) 29108

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Function(s)
protease binding interleukin-6 receptor binding protein binding tropomyosin binding cysteine-type endopeptidase activator activity involved in apoptotic process myosin I binding enzyme binding Pyrin domain binding identical protein binding protein homodimerization activity BMP receptor binding cysteine-type endopeptidase activity involved in apoptotic process
Process(es)
myeloid dendritic cell activation activation of innate immune response myeloid dendritic cell activation involved in immune response positive regulation of antigen processing and presentation of peptide antigen via MHC class II positive regulation of adaptive immune response apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response signal transduction regulation of tumor necrosis factor-mediated signaling pathway positive regulation of actin filament polymerization regulation of protein stability negative regulation of NF-kappaB transcription factor activity negative regulation of interferon-beta production positive regulation of interferon-gamma production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production tumor necrosis factor-mediated signaling pathway positive regulation of activated T cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of cysteine-type endopeptidase activity involved in apoptotic process macropinocytosis innate immune response positive regulation of JNK cascade positive regulation of interleukin-1 beta secretion positive regulation of phagocytosis defense response to Gram-negative bacterium positive regulation of T cell activation positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity defense response to virus positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor negative regulation of protein serine/threonine kinase activity intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of chemokine secretion positive regulation of release of cytochrome c from mitochondria positive regulation of T cell migration positive regulation of interleukin-8 secretion positive regulation of interleukin-6 secretion positive regulation of cysteine-type endopeptidase activity positive regulation of interleukin-10 secretion positive regulation of extrinsic apoptotic signaling pathway regulation of intrinsic apoptotic signaling pathway
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