Proteintech

PYCR2 Polyclonal Antibody

PYCR2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 19

PYCR2 Antibody (17146-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using PYCR2 antibody 17146-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

PYCR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PYCR2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17146-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PYCR2 Fusion Protein Ag10918 (1-320 aa encoded by BC020553)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSVGFIGAG QLAYALARGF TAAGILSAHK IIASSPEMNL PTVSALRKMG VNLTRSNKET VKHSDVLFLA VKPHIIPFIL DEIGADVQAR HIVVSCAAGV TISSVEKKLM AFQPAPKVIR CMTNTPVVVQ EGATVYATGT HALVEDGQLL EQLMSSVGFC TEVEEDLIDA VTGLSGSGPA YAFMALDALA DGGVKMGLPR RLAIQLGAQA LLGAAKMLLD SEQHPCQLKD NVCSPGGATI HALHFLESGG FRSLLINAVE ASCIRTRELQ SMADQEKISP AALKKTLLDR VKLESPTVST LTPSSPGKLL TRSLALGGKK D (1-320 aa encoded by BC020553)

Target Information

PYCR2 belongs to the pyrroline-5-carboxylate reductase family. The function of the PYCR2 protein is not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ALKBH5-PYCR2 Positive Feedback Loop Promotes Proneural-Mesenchymal Transition Via Proline Synthesis In GBM.

Journal of Cancer

Li L,Yang M,Pu X,Tang Y,Fei F,Li Z,Hou H,Chen Q,Wang Q,Wu Y,Zhang Y,Ren C,Gong A

Fri Sep 27 00:00:00 UTC 2024

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

EMBO molecular medicine

Southwell N,Primiano G,Nadkarni V,Attarwala N,Beattie E,Miller D,Alam S,Liparulo I,Shurubor YI,Valentino ML,Carelli V,Servidei S,Gross SS,Manfredi G,Chen Q,D'Aurelio M

Mon Jul 10 00:00:00 UTC 2023

Differential Degradation of TRA2A and PYCR2 Mediated by Ubiquitin E3 Ligase E4B.

Frontiers in cell and developmental biology

Lu Y,Jiang B,Peng K,Li S,Liu X,Wang B,Chen Y,Wang T,Zhao B

Sun Nov 05 00:00:00 UTC 2023

Proline synthesis through PYCR1 is required to support cancer cell proliferation and survival in oxygen-limiting conditions.

Cell reports

Westbrook RL,Bridges E,Roberts J,Escribano-Gonzalez C,Eales KL,Vettore LA,Walker PD,Vera-Siguenza E,Rana H,Cuozzo F,Eskla KL,Vellama H,Shaaban A,Nixon C,Luuk H,Lavery GG,Hodson DJ,Harris AL,Tennant DA

Tue Feb 01 00:00:00 UTC 2022

Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix.

Nature metabolism

Kay EJ,Paterson K,Riera-Domingo C,Sumpton D,Däbritz JHM,Tardito S,Boldrini C,Hernandez-Fernaud JR,Athineos D,Dhayade S,Stepanova E,Gjerga E,Neilson LJ,Lilla S,Hedley A,Koulouras G,McGregor G,Jamieson C,Johnson RM,Park M,Kirschner K,Miller C,Kamphorst JJ,Loayza-Puch F,Saez-Rodriguez J,Mazzone M,Blyth K,Zagnoni M,Zanivan S

Wed Jun 01 00:00:00 UTC 2022

PINCH-1 promotes Δ¹-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.

Amino acids

Cui C,Wang J,Guo L,Wu C

Wed Dec 01 00:00:00 UTC 2021

African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2.

Virologica Sinica

Chen S,Zhang X,Nie Y,Li H,Chen W,Lin W,Chen F,Xie Q

Thu Apr 01 00:00:00 UTC 2021

Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis.

The EMBO journal

Chen Z,Wang C,Feng X,Nie L,Tang M,Zhang H,Xiong Y,Swisher SK,Srivastava M,Chen J

Wed Sep 01 00:00:00 UTC 2021

Mitochondrial NADP⁺ is essential for proline biosynthesis during cell growth.

Nature metabolism

Tran DH,Kesavan R,Rion H,Soflaee MH,Solmonson A,Bezwada D,Vu HS,Cai F,Phillips JA,DeBerardinis RJ,Hoxhaj G

Thu Apr 01 00:00:00 UTC 2021

The upregulation of PYCR2 is associated with aggressive colon cancer progression and a poor prognosis.

Biochemical and biophysical research communications

Wang S,Gu L,Huang L,Fang J,Liu Z,Xu Q

Fri Oct 01 00:00:00 UTC 2021

The upregulation of PYCR2 is associated with aggressive colon cancer progression and a poor prognosis.

Biochemical and biophysical research communications

Wang S,Gu L,Huang L,Fang J,Liu Z,Xu Q

Fri Oct 01 00:00:00 UTC 2021

Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.

Scientific reports

Jones B,Balasubramaniam M,Lebowitz JJ,Taylor A,Villalta F,Khoshbouei H,Grueter C,Grueter B,Dash C,Pandhare J

Thu Jan 14 00:00:00 UTC 2021

Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.

The EMBO journal

Schwörer S,Berisa M,Violante S,Qin W,Zhu J,Hendrickson RC,Cross JR,Thompson CB

Wed Apr 15 00:00:00 UTC 2020

Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma.

Cell

Gao Q,Zhu H,Dong L,Shi W,Chen R,Song Z,Huang C,Li J,Dong X,Zhou Y,Liu Q,Ma L,Wang X,Zhou J,Liu Y,Boja E,Robles AI,Ma W,Wang P,Li Y,Ding L,Wen B,Zhang B,Rodriguez H,Gao D,Zhou H,Fan J

Thu Oct 03 00:00:00 UTC 2019

Kindlin-2 links mechano-environment to proline synthesis and tumor growth.

Nature communications

Guo L,Cui C,Zhang K,Wang J,Wang Y,Lu Y,Chen K,Yuan J,Xiao G,Tang B,Sun Y,Wu C

Tue Feb 19 00:00:00 UTC 2019

Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.

American journal of respiratory cell and molecular biology

Hamanaka RB,O'Leary EM,Witt LJ,Tian Y,Gökalp GA,Meliton AY,Dulin NO,Mutlu GM

Fri Nov 01 00:00:00 UTC 2019

A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder.

Aging and disease

Huang YW,Chiang MF,Ho CS,Hung PL,Hsu MH,Lee TH,Chu LJ,Liu H,Tang P,Victor Ng W,Lin DS

Sat Dec 01 00:00:00 UTC 2018

A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder.

Aging and disease

Huang YW,Chiang MF,Ho CS,Hung PL,Hsu MH,Lee TH,Chu LJ,Liu H,Tang P,Victor Ng W,Lin DS

Sat Dec 01 00:00:00 UTC 2018

Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.

Cell reports

Hollinshead KER,Munford H,Eales KL,Bardella C,Li C,Escribano-Gonzalez C,Thakker A,Nonnenmacher Y,Kluckova K,Jeeves M,Murren R,Cuozzo F,Ye D,Laurenti G,Zhu W,Hiller K,Hodson DJ,Hua W,Tomlinson IP,Ludwig C,Mao Y,Tennant DA

Tue Mar 20 00:00:00 UTC 2018

Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.

Cell reports

Tue Mar 20 00:00:00 UTC 2018

Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.

Scientific reports

Liu W,Hancock CN,Fischer JW,Harman M,Phang JM

Tue Nov 24 00:00:00 UTC 2015

Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.

Scientific reports

Liu W,Hancock CN,Fischer JW,Harman M,Phang JM

Tue Nov 24 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: P5C reductase 2; P5CR 2; pyrroline 5-carboxylate reductase isoform; Pyrroline-5-carboxylate reductase 2; pyrroline-5-carboxylate reductase family, member 2

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Gene Aliases: 1810018M05Rik; HLD10; P5CR2; PYCR2

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UniProt ID: (Human) Q96C36, (Mouse) Q922Q4, (Rat) Q6AY23

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Entrez Gene ID: (Human) 29920, (Mouse) 69051, (Rat) 364064

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Function(s)

pyrroline-5-carboxylate reductase activity oxidoreductase activity

Process(es)

proline biosynthetic process cellular amino acid biosynthetic process cellular response to oxidative stress oxidation-reduction process L-proline biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PYCR2 Polyclonal Antibody

PYCR2 Antibody (17146-1-AP) in ICC/IF

Product Details

17146-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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