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PZP Antibody (PA5-110249) in WB
Western blot analysis of PZP in extracts of various cell lines. Samples were incubated with PZP Polyclonal antibody (Product # PA5-110249) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-110249
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 620-740 of human PZP (NP_002855.2).
Conjugate
Form
Liquid
Concentration
0.92 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2855660
Product Specific Information
Immunogen sequence: VKDLTNFPDN VDQQEEEQGH CPRPFFIHNG AIYVPLSSNE ADIYSFLKGM GLKVFTNSKI RKPKSCSVIP SVSAGAVGQG YYGAGLGVVE RPYVPQLGTY NVIPLNNEQS SGPVPETVRS Y
Target Information
The protein encoded by this gene is highly expressed in late-pregnancy serum and is similar in structure to alpha-2-macroglobulin. The encoded protein, which acts as a homotetramer, inhibits the activity of all four classes of proteinases. This protein contains cleavage sites for several proteinases. Upon binding of a proteinase, the conformation of this protein changes to trap the proteinase, limiting its activity. This protein appears to be elevated in the sera of presymptomatic Alzheimer's disease patients.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: A1m {ECO:0000312|EMBL:AAA40723.1}; alpha 1 macroglobulin; Alpha-1-M; Alpha-1-macroglobulin; Alpha-1-macroglobulin 165 kDa subunit; alpha-2-M; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6; Pregnancy zone protein; pregnancy-zone protein
Gene Aliases: A1m; A2m; AI893533; CPAMD6; MAM; Pzp
UniProt ID: (Rat) Q63041
Entrez Gene ID: (Human) 5858, (Mouse) 11287, (Rat) 252922
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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