Invitrogen

Pan Ras Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers

Product Details

PA5-78035

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:10,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-
Product Specifications

Species Reactivity

Amphibian, Goat, Human, Mouse, Rodent, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KRAS. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2736317

Product Specific Information

Positive Control: A549, H1299, HCT116, KRAS, HRAS, NRAS, zebrafish liver, zebrafish GI, whole zebrafish

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: c-H-ras; c-Ha-ras p21 protein; c-Ha-ras transgene; c-has/bas p21 protein; c-K-ras; c-K-ras2 protein; c-Ki-ras; c-Kirsten-ras protein; c-Kirsten-ras proto-oncogene; c-ras-Ki-2 activated oncogene; cellular c-Ki-ras2; cellular c-Ki-ras2 proto-oncogene; GTP- and GDP-binding peptide B; GTPase HRas; GTPase Hras1; GTPase KRas; GTPase NRas; H-ras 1 protein; H-Ras-1; Ha-Ras; Ha-Ras1 proto-oncoprotein; Harvey ras1 protein; Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; Harvey rat sarcoma virus oncogene 1; K RAS; K-Ras 2; K-ras p21 protein; Ki-Ras; Kirsten rat sarcoma oncogene 2, expressed; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral oncogene homologue 2 (active); N-ras oncogene p21; N-ras protein part 4; Neuroblastoma RAS; neuroblastoma RAS viral (v-ras) oncogene homolog; oncogene KRAS2; p19 H-RasIDX protein; p21 protein; p21ras; PR310 c-K-ras oncogene; Ras family small GTP binding protein H-Ras; ras-like protein; RASK; RASN; transformation gene: oncogene HAMSV; Transforming protein N-Ras; Transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog; v-ras neuroblastoma RAS viral oncogene homolog; xRAS1

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Gene Aliases: AI929937; ALPS4; AV095280; C-BAS/HAS; C-H-RAS; c-Ha-ras; C-HA-RAS1; C-K-RAS; c-Ki-ras; c-rasHa; CFC2; CMNS; CTLO; etID18709.8; fa04e08; fc14b12; fc23g10; fj89d12; fj99a10; H-ras; H-RASIDX; Ha-ras; HAMSV; Harvey-ras; HRAS; Hras-1; HRAS1; K-ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS; Kras-2; KRAS1; KRAS2; N-ras; NCMS; NRAS; NRAS1; NS; NS3; NS6; p21; p21B; p21ras; RALD; ras; RASH1; RASK2; sb:eu826; wu:fa04e08; wu:fc14b12; wu:fc23g10; wu:fj89d12; wu:fj99a10; xRAS2; zgc:100761; zgc:110250; zgc:85725; zras-b1

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UniProt ID: (Human) P01112, (Mouse) Q61411, (Rat) P20171, (Human) P01116, (Rat) P08644, (Mouse) P32883, (Human) P01111, (Rat) Q04970

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Entrez Gene ID: (Human) 3265, (Mouse) 15461, (Rat) 293621, (Human) 3845, (Rat) 24525, (Mouse) 16653, (Human) 4893, (Mouse) 18176, (Rat) 24605, (Zebrafish) 550286, (Zebrafish) 445289, (Zebrafish) 30380

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Function(s)
GTPase activity protein binding GTP binding protein C-terminus binding nucleotide binding GMP binding GDP binding LRR domain binding protein complex binding molecular_function
Process(es)
MAPK cascade stimulatory C-type lectin receptor signaling pathway epidermal growth factor receptor signaling pathway Ras protein signal transduction axon guidance Fc-epsilon receptor signaling pathway ERBB2 signaling pathway leukocyte migration establishment of protein localization to Golgi regulation of protein phosphorylation signal transduction small GTPase mediated signal transduction regulation of gene expression positive regulation of fibroblast proliferation negative regulation of skeletal muscle tissue development regulation of cell cycle positive regulation of protein phosphorylation positive regulation of cell proliferation visual learning positive regulation of gene expression cytokine-mediated signaling pathway forebrain astrocyte development actin cytoskeleton organization regulation of protein stability regulation of synaptic transmission, GABAergic positive regulation of Rac protein signal transduction social behavior endocrine signaling positive regulation of MAP kinase activity negative regulation of neuron apoptotic process negative regulation of cell differentiation regulation of long-term neuronal synaptic plasticity homeostasis of number of cells within a tissue positive regulation of nitric-oxide synthase activity positive regulation of NF-kappaB transcription factor activity striated muscle cell differentiation response to glucocorticoid response to mineralocorticoid epithelial tube branching involved in lung morphogenesis endocytosis apoptotic process cell cycle arrest mitotic cell cycle checkpoint cell aging cell proliferation negative regulation of cell proliferation negative regulation of gene expression positive regulation of cell migration negative regulation of GTPase activity positive regulation of MAPK cascade positive regulation of GTPase activity positive regulation of DNA replication positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of Ras protein signal transduction positive regulation of epithelial cell proliferation protein heterooligomerization positive regulation of ERK1 and ERK2 cascade positive regulation of wound healing cellular senescence intrinsic apoptotic signaling pathway positive regulation of ruffle assembly positive regulation of actin cytoskeleton reorganization positive regulation of miRNA metabolic process chemotaxis cell surface receptor signaling pathway organ morphogenesis positive regulation of interferon-gamma production T-helper 1 type immune response defense response to protozoan ephrin receptor signaling pathway T cell receptor signaling pathway biological_process heart development hemopoiesis embryonic cranial skeleton morphogenesis
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