Invitrogen

Pannexin 1 Polyclonal Antibody

Pannexin 1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

FIGURE: 1 / 9

Pannexin 1 Antibody (488100) in WB, ICC/IF

Channels (Austin, Tex.) 2015 - Figure 3. Panx1 channels are involved in epiplexus cells activation by exogenous 100 uM ATP. Panx1 channels are robustly expressed on choroidal epithelium, but were rarely detected in epiplexus cells. Western blotting analysis confirmed presence of Panx1 in the CP. The Panx1 blockers, 500 uM probenecid and 100 uM 10 panx significantly decreased ATP-triggered chemokinesis. ( A and B ) Immunofluorescent stain... View More

Pannexin 1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Pannexin 1 Antibody in Immunohistochemistry (IHC)

Pannexin 1 Antibody in Western Blot (WB)

Product Image Gallery

Product Details

488100

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

2-4 µg/mL

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the mouse Pannexin 1 protein (Accession# NP_062355), which is identical to rat and 92% homologous to human sequence.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2532255

Target Information

The pannexin gene family encodes a second class of putative gap junction proteins and are highly conserved in invertebrates and mammals. Pannexins (Panx) are four-pass transmembrane proteins that oligomerize to form large pore ion and metabolite-permeable channels. Pannexin-1 (PANX1) and Pannexin-3 are closely related, while Pannexin-2 is a more distant relation. PANX1 is a transmembrane protein that forms a mechanosensitive ATP-permeable channel between adjacent cells and in the endoplasmic reticulum. PANX1 may play a role as a Ca2+ -leak channel to regulate ER Ca2+ homeostasis and regulates neural stem and progenitor cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Convergent NMDA receptor-Pannexin1 signaling pathways regulate the interaction of CaMKII with Connexin-36.

Communications biology

Siu RCF,Kotova A,Timonina K,Zoidl C,Zoidl GR

488100 was used in Cell Culture to demonstrate that the efficacy of Cx36 channels requires convergent calcium-dependent signaling processes in which activation of ionotropic N-methyl-D-aspartate receptor, Src-family kinase, and Pannexin1 open Cx36.

Tue Jun 08 00:00:00 UTC 2021

Convergent NMDA receptor-Pannexin1 signaling pathways regulate the interaction of CaMKII with Connexin-36.

Communications biology

Siu RCF,Kotova A,Timonina K,Zoidl C,Zoidl GR

Tue Jun 08 00:00:00 UTC 2021

IκB kinase inhibition remodeled connexins, pannexin-1, and excitatory amino-acid transporters expressions to promote neuroprotection of galantamine and morphine.

Journal of cellular physiology

Magdy S,Gamal M,Samir NF,Rashed L,Emad Aboulhoda B,Mohammed HS,Sharawy N

488100 was used in Immunohistochemistry to conclude that NF-κB is an important component of reshaping the expressions of Cxs, panx-1, and EAATs and the development of glutamate-induced neuronal degeneration.

Mon Nov 01 00:00:00 UTC 2021

Neuronal pannexin-1 channels are not molecular routes of water influx during spreading depolarization-induced dendritic beading.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Sword J,Croom D,Wang PL,Thompson RJ,Kirov SA

488100 was used in immunohistochemistry to study the spreading depolarization-induced dendritic beading where neuronal pannexin-1 channels are not molecular routes of water influx

Mon May 01 00:00:00 UTC 2017

Dual Oxidase 2 (Duox2) Regulates Pannexin 1-mediated ATP Release in Primary Human Airway Epithelial Cells via Changes in Intracellular pH and Not H2O2 Production.

The Journal of biological chemistry

Krick S,Wang J,St-Pierre M,Gonzalez C,Dahl G,Salathe M

488100 was used in western blot to determine the role of Duox2 and how it regulates Pannexin 1-mediated ATP release in primary human airway epithelial cells via changes in intracellular pH and not H202 production

Fri Mar 18 00:00:00 UTC 2016

A critical role for pannexin-1 in activation of innate immune cells of the choroid plexus.

Channels (Austin, Tex.)

Maslieieva V,Thompson RJ

488100 was used in immunocytochemistry and western blot to study pannexin-1 and it role in the activation of innate immune cells of the choroid plexus

Mon Jan 05 00:00:00 UTC 2015

A critical role for pannexin-1 in activation of innate immune cells of the choroid plexus.

Channels (Austin, Tex.)

Maslieieva V,Thompson RJ

488100 was used in immunocytochemistry and western blot to study pannexin-1 and it role in the activation of innate immune cells of the choroid plexus

Mon Jan 05 00:00:00 UTC 2015

A critical role for pannexin-1 in activation of innate immune cells of the choroid plexus.

Channels (Austin, Tex.)

Maslieieva V,Thompson RJ

488100 was used in immunocytochemistry and western blot to study pannexin-1 and it role in the activation of innate immune cells of the choroid plexus

Mon Jan 05 00:00:00 UTC 2015

Abundant expression and functional participation of TRPV1 at Zusanli acupoint (ST36) in mice: mechanosensitive TRPV1 as an "acupuncture-responding channel".

BMC complementary and alternative medicine

Wu SY,Chen WH,Hsieh CL,Lin YW

488100 was used in Western Blotting to demonstrate that the mechanosensitive channel TRPV1 is highly expressed at ST36 and possibly participates in acupuncture related analgesia.

Tue Mar 11 00:00:00 UTC 2014

Anoxia-induced NMDA receptor activation opens pannexin channels via Src family kinases.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Weilinger NL,Tang PL,Thompson RJ

488100 was used in immunohistochemistry to identify mechanisms of Panx activation.

Wed Sep 05 00:00:00 UTC 2012

Heightened inflammasome activation is linked to age-related cognitive impairment in Fischer 344 rats.

BMC neuroscience

Mawhinney LJ,de Rivero Vaccari JP,Dale GA,Keane RW,Bramlett HM

488100 was used in immunohistochemistry - frozen section and western blot to discuss the role of NLRP/NALP during central nervous system injury.

Thu Dec 01 00:00:00 UTC 2011

Heightened inflammasome activation is linked to age-related cognitive impairment in Fischer 344 rats.

BMC neuroscience

Mawhinney LJ,de Rivero Vaccari JP,Dale GA,Keane RW,Bramlett HM

488100 was used in immunohistochemistry - frozen section and western blot to discuss the role of NLRP/NALP during central nervous system injury.

Thu Dec 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: Pannexin-1

View more View less

Gene Aliases: AI847747; Panx1

View more View less

UniProt ID: (Mouse) Q9JIP4

View more View less

Entrez Gene ID: (Mouse) 55991

View more View less

Function(s)

protease binding actin binding receptor binding gap junction channel activity calcium channel activity protein binding channel activity wide pore channel activity leak channel activity ion channel binding protein heterodimerization activity actin filament binding gap junction hemi-channel activity scaffold protein binding

Process(es)

response to ischemia transport ion transport cation transport calcium ion transport cell-cell signaling response to ATP protein hexamerization positive regulation of cytokine secretion positive regulation of interleukin-1 alpha secretion positive regulation of interleukin-1 beta secretion transmembrane transport calcium ion transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Pannexin 1 Polyclonal Antibody

Pannexin 1 Antibody (488100) in WB, ICC/IF

Product Details

488100

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!