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Pat1b Antibody (A303-482A) in WB
Detection of human Pat1b by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Pat1b antibody (Product # A303-482A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. Pat1b was also immunoprecipitated by rabbit anti-Pat1b antibodies BL12431 and BL12432, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 min... View More
Product Details
A303-482A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 425 and 475 of human Protein Associated with Topoisomerase II Homolog 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Orangutan
Target Information
RNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Acts as a scaffold protein that connects deadenylation and decapping machinery. Required for cytoplasmic mRNA processing body (P-body) assembly. In case of infection, required for translation and replication of hepatitis C virus (HCV).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PAT1-like protein 1; Pat1b; protein associated with topoisomerase II homolog 1; Protein PAT1 homolog 1; Protein PAT1 homolog b
Gene Aliases: hPat1b; OK/KNS-cl.5; Pat1b; PATL1
UniProt ID: (Human) Q86TB9
Entrez Gene ID: (Human) 219988
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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