Invitrogen

Paxillin Polyclonal Antibody

Paxillin Antibody in Immunocytochemistry (ICC/IF)

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Paxillin Antibody (PA5-79899) in ICC/IF

Immunocytochemistry analysis of PXN using anti-PXN antibody (Product # PA5-79899) . PXN was detected in a section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-PXN antibody (Product # PA5-79899) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize... View More

Paxillin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Paxillin Antibody in Immunohistochemistry (Frozen) (IHC (F))

Paxillin Antibody in Flow Cytometry (Flow)

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Product Details

PA5-79899

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

0.5-1 µg/mL

Immunocytochemistry (ICC/IF)

0.5-1 µg/mL

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminal of human Paxillin (456-472aa HEKDGKAYCRKDYFDMF).

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.01mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2747014

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human 293T whole cell, human HepG2 whole cell, human HEL whole cell, rat brain tissue, mouse brain tissue. IHC: Human Lung Cancer tissue IHC-F: Rat Kidney tissue. ICC/IF: PC-3 cell, Hela cell. Flow: A549 cell.

Target Information

Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Vaccine against gastrin, a polyclonal antibody stimulator, decreases pancreatic cancer metastases.

American journal of physiology. Gastrointestinal and liver physiology

Osborne N,Sundseth R,Gay MD,Cao H,Tucker RD,Nadella S,Wang S,Liu X,Kroemer A,Sutton L,Cato A,Smith JP

PA5-79899 was used in Western Blotting to investigate the effects of lone and combined treatment of polyclonal antibody stimulator, an anti-gastrin cancer vaccine, on pancreatic cancer growth, metastases, and the tumor microenvironment.

Fri Nov 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: FLJ16691; hCG_1778014; myocardial ischemic preconditioning associated protein 7; PAX-1; PAXI; Paxillin; paxillin alpha; testicular tissue protein Li 134

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Gene Aliases: AW108311; AW123232; Pax; PXN

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UniProt ID: (Human) P49023, (Mouse) Q8VI36

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Entrez Gene ID: (Human) 5829, (Mouse) 19303, (Rat) 360820

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Function(s)

integrin binding protein binding beta-catenin binding zinc ion binding vinculin binding protein kinase binding receptor signaling complex scaffold activity protein complex binding metal ion binding BH4 domain binding

Process(es)

activation of MAPK activity movement of cell or subcellular component cytoskeleton organization cell adhesion cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway regulation of cell shape peptidyl-tyrosine phosphorylation lamellipodium assembly substrate adhesion-dependent cell spreading positive regulation of protein kinase activity focal adhesion assembly branching morphogenesis of an epithelial tube growth hormone receptor signaling pathway muscle contraction signal transduction signal complex assembly epidermal growth factor receptor signaling pathway cellular response to reactive oxygen species vascular endothelial growth factor receptor signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Paxillin Polyclonal Antibody

Paxillin Antibody (PA5-79899) in ICC/IF

Product Details

PA5-79899

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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