Invitrogen

Paxillin Monoclonal Antibody (5H11)

Paxillin Antibody in Immunocytochemistry (ICC/IF)

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Paxillin Antibody (AHO0492) in ICC/IF

Immunofluorescence analysis of Paxillin was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Paxillin (5H11) Mouse Monoclonal Antibody (Product # AHO0492) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)... View More

Paxillin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Paxillin Antibody in Immunoprecipitation (IP)

Paxillin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Paxillin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Paxillin Antibody in Immunohistochemistry (IHC)

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Product Details

AHO0492

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

Immunocytochemistry (ICC/IF)

2 µg/mL

View 14 publications 14 publications

Immunoprecipitation (IP)

2 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

5H11

Immunogen

Purified recombinant full-length human paxillin protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2536312

Product Specific Information

Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissues in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at room temperature for 20 min. Recommended positive controls include HeLa cells and colon carcinoma.

Target Information

Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer.

Science advances

Mohanty A,Nam A,Srivastava S,Jones J,Lomenick B,Singhal SS,Guo L,Cho H,Li A,Behal A,Mirzapoiazova T,Massarelli E,Koczywas M,Arvanitis LD,Walser T,Villaflor V,Hamilton S,Mambetsariev I,Sattler M,Nasser MW,Jain M,Batra SK,Soldi R,Sharma S,Fakih M,Mohanty SK,Mainan A,Wu X,Chen Y,He Y,Chou TF,Roy S,Orban J,Kulkarni P,Salgia R

Published figure using Paxillin monoclonal antibody (Product # AHO0492) in Western Blot

Fri Oct 13 00:00:00 UTC 2023

The Lipid-Binding Defective Dynamin 2 Mutant in Charcot-Marie-Tooth Disease Impairs Proper Actin Bundling and Actin Organization in Glomerular Podocytes.

Frontiers in cell and developmental biology

Hamasaki E,Wakita N,Yasuoka H,Nagaoka H,Morita M,Takashima E,Uchihashi T,Takeda T,Abe T,Lee JW,Iimura T,Saleem MA,Ogo N,Asai A,Narita A,Takei K,Yamada H

Published figure using Paxillin monoclonal antibody (Product # AHO0492) in Immunocytochemistry

Sat Jul 16 00:00:00 UTC 2022

Tumor cell-conditioned media drives collagen remodeling via fibroblast and pericyte activation in an in vitro premetastatic niche model.

iScience

Nairon KG,DePalma TJ,Zent JM,Leight JL,Skardal A

Published figure using Paxillin monoclonal antibody (Product # AHO0492) in Immunocytochemistry

Fri Jul 15 00:00:00 UTC 2022

mTORC1 activity is supported by spatial association with focal adhesions.

The Journal of cell biology

Rabanal-Ruiz Y,Byron A,Wirth A,Madsen R,Sedlackova L,Hewitt G,Nelson G,Stingele J,Wills JC,Zhang T,Zeug A,Fässler R,Vanhaesebroeck B,Maddocks ODK,Ponimaskin E,Carroll B,Korolchuk VI

Published figure using Paxillin monoclonal antibody (Product # AHO0492) in Immunocytochemistry

Mon May 03 00:00:00 UTC 2021

Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion.

Nature communications

Junqueira Alves C,Dariolli R,Haydak J,Kang S,Hannah T,Wiener RJ,DeFronzo S,Tejero R,Gusella GL,Ramakrishnan A,Alves Dias R,Wojcinski A,Kesari S,Shen L,Sobie EA,Rodrigues Furtado de Mendonça JP,Azeloglu EU,Zou H,Friedel RH

Published figure using Paxillin monoclonal antibody (Product # AHO0492) in Immunocytochemistry

Thu Oct 14 00:00:00 UTC 2021

Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion.

Nature communications

Published figure using Paxillin monoclonal antibody (Product # AHO0492) in Immunocytochemistry

Thu Oct 14 00:00:00 UTC 2021

Inhibiting FAK-Paxillin Interaction Reduces Migration and Invadopodia-Mediated Matrix Degradation in Metastatic Melanoma Cells.

Cancers

Mousson A,Legrand M,Steffan T,Vauchelles R,Carl P,Gies JP,Lehmann M,Zuber G,De Mey J,Dujardin D,Sick E,Rondé P

Published figure using Paxillin monoclonal antibody (Product # AHO0492) in Immunocytochemistry

Wed Apr 14 00:00:00 UTC 2021

Inhibiting FAK-Paxillin Interaction Reduces Migration and Invadopodia-Mediated Matrix Degradation in Metastatic Melanoma Cells.

Cancers

Mousson A,Legrand M,Steffan T,Vauchelles R,Carl P,Gies JP,Lehmann M,Zuber G,De Mey J,Dujardin D,Sick E,Rondé P

Published figure using Paxillin monoclonal antibody (Product # AHO0492) in Immunocytochemistry

Wed Apr 14 00:00:00 UTC 2021

Nup93 regulates breast tumor growth by modulating cell proliferation and actin cytoskeleton remodeling.

Life science alliance

Bersini S,Lytle NK,Schulte R,Huang L,Wahl GM,Hetzer MW

Published figure using Paxillin monoclonal antibody (Product # AHO0492) in Immunocytochemistry

Wed Jan 01 00:00:00 UTC 2020

Fibronectin Patches as Anchoring Points for Force Sensing and Transmission in Human Induced Pluripotent Stem Cell-Derived Pericytes.

Stem cell reports

Iendaltseva O,Orlova VV,Mummery CL,Danen EHJ,Schmidt T

Published figure using Paxillin monoclonal antibody (Product # AHO0492) in Immunocytochemistry

Tue Jun 09 00:00:00 UTC 2020

Adenomatous polyposis coli nucleates actin assembly to drive cell migration and microtubule-induced focal adhesion turnover.

The Journal of cell biology

Juanes MA,Bouguenina H,Eskin JA,Jaiswal R,Badache A,Goode BL

Published figure using Paxillin monoclonal antibody (Product # AHO0492) in Western Blot

Mon Sep 04 00:00:00 UTC 2017

Vinexin family (SORBS) proteins play different roles in stiffness-sensing and contractile force generation.

Journal of cell science

Ichikawa T,Kita M,Matsui TS,Nagasato AI,Araki T,Chiang SH,Sezaki T,Kimura Y,Ueda K,Deguchi S,Saltiel AR,Kioka N

AHO0492 was used in Western Blotting to study the differential roles of vinexin family proteins in mechanotransduction.

Sun Oct 15 00:00:00 UTC 2017

Vinexin family (SORBS) proteins play different roles in stiffness-sensing and contractile force generation.

Journal of cell science

Ichikawa T,Kita M,Matsui TS,Nagasato AI,Araki T,Chiang SH,Sezaki T,Kimura Y,Ueda K,Deguchi S,Saltiel AR,Kioka N

AHO0492 was used in Western Blotting to study the differential roles of vinexin family proteins in mechanotransduction.

Sun Oct 15 00:00:00 UTC 2017

Nicotinic acid impairs assembly of leading edge in glioma cells.

Oncology reports

Yang X,Mei S,Niu H,Li J

Published figure using Paxillin monoclonal antibody (Product # AHO0492) in Immunocytochemistry

Tue Aug 01 00:00:00 UTC 2017

Nicotinic acid impairs assembly of leading edge in glioma cells.

Oncology reports

Yang X,Mei S,Niu H,Li J

Published figure using Paxillin monoclonal antibody (Product # AHO0492) in Immunocytochemistry

Tue Aug 01 00:00:00 UTC 2017

The Arf activator GBF1 localizes to plasma membrane sites involved in cell adhesion and motility.

Cellular logistics

Busby T,Meissner JM,Styers ML,Bhatt J,Kaushik A,Hjelmeland AB,Sztul E

AHO0492 was used in Immunocytochemistry-immunoflourescence to investigate the localisation of GBF1 in cell lines derived from human glioblastomas.

Thu Feb 10 00:00:00 UTC 2022

High cell-surface density of HER2 deforms cell membranes.

Nature communications

Chung I,Reichelt M,Shao L,Akita RW,Koeppen H,Rangell L,Schaefer G,Mellman I,Sliwkowski MX

AHO0492 was used in immunocytochemistry and western blot describe the effects of HER2 overexpression in breast cancer cells

Wed Sep 07 00:00:00 UTC 2016

Paxillin mutations affect focal adhesions and lead to altered mitochondrial dynamics: relevance to lung cancer.

Cancer biology & therapy

Kawada I,Hasina R,Lennon FE,Bindokas VP,Usatyuk P,Tan YH,Krishnaswamy S,Arif Q,Carey G,Hseu RD,Robinson M,Tretiakova M,Brand TM,Iida M,Ferguson MK,Wheeler DL,Husain AN,Natarajan V,Vokes EE,Singleton PA,Salgia R

AHO0492 was used in immunocytochemistry and western blot to examine the effects of activating mutations of PXN on mitochondrial function.

Mon Jul 01 00:00:00 UTC 2013

Paxillin mutations affect focal adhesions and lead to altered mitochondrial dynamics: relevance to lung cancer.

Cancer biology & therapy

AHO0492 was used in immunocytochemistry and western blot to examine the effects of activating mutations of PXN on mitochondrial function.

Mon Jul 01 00:00:00 UTC 2013

αV-integrins are required for mechanotransduction in MDCK epithelial cells.

PloS one

Teräväinen TP,Myllymäki SM,Friedrichs J,Strohmeyer N,Moyano JV,Wu C,Matlin KS,Muller DJ,Manninen A

AHO0492 was used in immunocytochemistry to demonstrate that αV-integrins regulate the maturation of focal adhesions and cell spreading.

Tue Apr 08 00:00:00 UTC 2014

αV-integrins are required for mechanotransduction in MDCK epithelial cells.

PloS one

Teräväinen TP,Myllymäki SM,Friedrichs J,Strohmeyer N,Moyano JV,Wu C,Matlin KS,Muller DJ,Manninen A

AHO0492 was used in immunocytochemistry to demonstrate that αV-integrins regulate the maturation of focal adhesions and cell spreading.

Tue Apr 08 00:00:00 UTC 2014

c-Yes regulates cell adhesion at the blood-testis barrier and the apical ectoplasmic specialization in the seminiferous epithelium of rat testes.

The international journal of biochemistry & cell biology

Xiao X,Mruk DD,Lee WM,Cheng CY

AHO0492 was used in western blot to elucidate how c-Yes regulates the blood-testis barrier and apical ectoplasmic specialization integrity.

Fri Apr 01 00:00:00 UTC 2011

Presenilin 1 affects focal adhesion site formation and cell force generation via c-Src transcriptional and posttranslational regulation.

The Journal of biological chemistry

Waschbüsch D,Born S,Niediek V,Kirchgessner N,Tamboli IY,Walter J,Merkel R,Hoffmann B

AHO0492 was used in western blot to assess the function of presenilins in the cell-matrix interactions of mouse embryonic fibroblasts

Fri Apr 10 00:00:00 UTC 2009

Loss of caveolin-1 polarity impedes endothelial cell polarization and directional movement.

The Journal of biological chemistry

Beardsley A,Fang K,Mertz H,Castranova V,Friend S,Liu J

Fri Feb 04 00:00:00 UTC 2005

Activation of platelet-activating factor receptor-coupled G alpha q leads to stimulation of Src and focal adhesion kinase via two separate pathways in human umbilical vein endothelial cells.

The Journal of biological chemistry

Deo DD,Bazan NG,Hunt JD

AHO0492 was used in Immunoprecipitation to demonstrate that platelet-activating factor activates pertussis toxin-insensitive G alpha(q) protein upon binding to its seven transmembrane receptor.

Fri Jan 30 00:00:00 UTC 2004

Aberrant activation of focal adhesion proteins mediates fibrillar amyloid beta-induced neuronal dystrophy.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Grace EA,Busciglio J

Wed Jan 15 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: FLJ16691; hCG_1778014; myocardial ischemic preconditioning associated protein 7; PAX-1; PAXI; Paxillin; paxillin alpha; testicular tissue protein Li 134

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Gene Aliases: AW108311; AW123232; Pax; PXN

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UniProt ID: (Human) P49023, (Mouse) Q8VI36

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Entrez Gene ID: (Human) 5829, (Mouse) 19303, (Rat) 360820

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Function(s)

integrin binding protein binding beta-catenin binding zinc ion binding vinculin binding protein kinase binding receptor signaling complex scaffold activity protein complex binding metal ion binding BH4 domain binding

Process(es)

activation of MAPK activity movement of cell or subcellular component cytoskeleton organization cell adhesion cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway regulation of cell shape peptidyl-tyrosine phosphorylation lamellipodium assembly substrate adhesion-dependent cell spreading positive regulation of protein kinase activity focal adhesion assembly branching morphogenesis of an epithelial tube growth hormone receptor signaling pathway muscle contraction signal transduction signal complex assembly epidermal growth factor receptor signaling pathway cellular response to reactive oxygen species vascular endothelial growth factor receptor signaling pathway

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Paxillin Monoclonal Antibody (5H11)

Paxillin Antibody (AHO0492) in ICC/IF

Product Details

AHO0492

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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