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Precautions should be taken upon storage for longer periods. Problems of long term stability may occur with highly diluted solutions. No other preservative agent has been added to the present formulation. For long storage purposes in solution the addition of sodium azide at 0.02 % is advised with the appropriate precautions of use.
Antibody can be used in ELISA and coupled to column-material for IAC. Depending on conjugate: dilution 2,000 from the delivered solution. (The titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). The antibody is applicable for any type of (waste) water sample. The detection limit is 2.5 µg/l, while the end concentration is 100 µg/l. The standard curve runs from 5-100 µg/l. For an optimal ELISA result it is crucial to use Greiner high affinity plates; As assaybuffer 0,1M PBS, 1% BSA heat-treated, pH 7,4 is recommended.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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