Invitrogen

Periostin Polyclonal Antibody

Periostin Antibody in Immunocytochemistry (ICC/IF)

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Periostin Antibody (PA5-34641) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Periostin in paraformaldehyde-fixed HeLa cells using Periostin Polyclonal Antibody (Product # PA5-34641) at a dilution of 1:200.

Periostin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Periostin Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-34641

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Periostin. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2551993

Product Specific Information

Recommended positive controls: HeLa, A431, H1299, HeLaS3, NIH-3T3.

Predicted reactivity: Pig (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor beta 1(TGF beta 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Single-cell analysis of hepatoblastoma identifies tumor signatures that predict chemotherapy susceptibility using patient-specific tumor spheroids.

Nature communications

Song H,Bucher S,Rosenberg K,Tsui M,Burhan D,Hoffman D,Cho SJ,Rangaswami A,Breese M,Leung S,Ventura MVP,Sweet-Cordero EA,Huang FW,Nijagal A,Wang B

Published figure using Periostin polyclonal antibody (Product # PA5-34641) in Immunohistochemistry

Thu Aug 25 00:00:00 UTC 2022

Caesalpinia sappan Linn. Ameliorates Allergic Nasal Inflammation by Upregulating the Keap1/Nrf2/HO-1 Pathway in an Allergic Rhinitis Mouse Model and Nasal Epithelial Cells.

Antioxidants (Basel, Switzerland)

Pyun BJ,Jo K,Lee JY,Lee A,Jung MA,Hwang YH,Jung DH,Ji KY,Choi S,Kim YH,Kim T

PA5-34641 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to exhibit that CSLW treatment significantly reduces infiltration of eosinophils and production of periostin, MUC5AC, and intracellular ROS through the Keap1/Nrf2/HO-1 pathway in HNEpCs.

Tue Nov 15 00:00:00 UTC 2022

Caesalpinia sappan Linn. Ameliorates Allergic Nasal Inflammation by Upregulating the Keap1/Nrf2/HO-1 Pathway in an Allergic Rhinitis Mouse Model and Nasal Epithelial Cells.

Antioxidants (Basel, Switzerland)

Pyun BJ,Jo K,Lee JY,Lee A,Jung MA,Hwang YH,Jung DH,Ji KY,Choi S,Kim YH,Kim T

Tue Nov 15 00:00:00 UTC 2022

Gardenia jasminoides Attenuates Allergic Rhinitis-Induced Inflammation by Inhibiting Periostin Production.

Pharmaceuticals (Basel, Switzerland)

Pyun BJ,Lee JY,Kim YJ,Ji KY,Jung DH,Park KS,Jo K,Choi S,Jung MA,Kim YH,Kim T

PA5-34641 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to investigate the effects of extracts from Gardenia jasminoides on allergic rhinitis-associated inflammatory responses that cause epithelial damage.

Tue Sep 28 00:00:00 UTC 2021

Gardenia jasminoides Attenuates Allergic Rhinitis-Induced Inflammation by Inhibiting Periostin Production.

Pharmaceuticals (Basel, Switzerland)

Pyun BJ,Lee JY,Kim YJ,Ji KY,Jung DH,Park KS,Jo K,Choi S,Jung MA,Kim YH,Kim T

Tue Sep 28 00:00:00 UTC 2021

Gardenia jasminoides Attenuates Allergic Rhinitis-Induced Inflammation by Inhibiting Periostin Production.

Pharmaceuticals (Basel, Switzerland)

Pyun BJ,Lee JY,Kim YJ,Ji KY,Jung DH,Park KS,Jo K,Choi S,Jung MA,Kim YH,Kim T

Tue Sep 28 00:00:00 UTC 2021

Colocalization of Erythrocytes and Vascular Calcification in Human Atherosclerosis: A Systematic Histomorphometric Analysis.

TH open : companion journal to thrombosis and haemostasis

Böhm EW,Pavlaki M,Chalikias G,Mikroulis D,Georgiadis GS,Tziakas DN,Konstantinides S,Schäfer K

Published figure using Periostin polyclonal antibody (Product # PA5-34641) in Immunohistochemistry

Thu Apr 01 00:00:00 UTC 2021

miR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma.

BioMed research international

Chen K,Li Z,Zhang M,Wang B,Peng T,Shen Y,Zhang J,Ye J,Liu Y,Tang D,Peng M,Ma D,Xiao Z,Zhang Y,Jin W,Li X

PA5-34641 was used in Western Blot, Immunohistochemistry to identify miR-876 and its target, POSTN, as potential therapeutic targets or prognostic markers of hepatocellular carcinoma due to their impact on EMT and liver fibrosis.

Wed May 05 00:00:00 UTC 2021

miR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma.

BioMed research international

Chen K,Li Z,Zhang M,Wang B,Peng T,Shen Y,Zhang J,Ye J,Liu Y,Tang D,Peng M,Ma D,Xiao Z,Zhang Y,Jin W,Li X

Wed May 05 00:00:00 UTC 2021

miR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma.

BioMed research international

Chen K,Li Z,Zhang M,Wang B,Peng T,Shen Y,Zhang J,Ye J,Liu Y,Tang D,Peng M,Ma D,Xiao Z,Zhang Y,Jin W,Li X

Wed May 05 00:00:00 UTC 2021

miR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma.

BioMed research international

Chen K,Li Z,Zhang M,Wang B,Peng T,Shen Y,Zhang J,Ye J,Liu Y,Tang D,Peng M,Ma D,Xiao Z,Zhang Y,Jin W,Li X

Wed May 05 00:00:00 UTC 2021

Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch.

Cell reports

Mishra SK,Wheeler JJ,Pitake S,Ding H,Jiang C,Fukuyama T,Paps JS,Ralph P,Coyne J,Parkington M,DeBrecht J,Ehrhardt-Humbert LC,Cruse GP,Bäumer W,Ji RR,Ko MC,Olivry T

PA5-34641 was used in Western Blotting to show that periostin, abundantly expressed in the skin of patients with atopic dermatitis (AD), induces itch in mice, dogs, and monkeys.

Tue Apr 07 00:00:00 UTC 2020

Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch.

Cell reports

Mishra SK,Wheeler JJ,Pitake S,Ding H,Jiang C,Fukuyama T,Paps JS,Ralph P,Coyne J,Parkington M,DeBrecht J,Ehrhardt-Humbert LC,Cruse GP,Bäumer W,Ji RR,Ko MC,Olivry T

PA5-34641 was used in Western Blotting to show that periostin, abundantly expressed in the skin of patients with atopic dermatitis (AD), induces itch in mice, dogs, and monkeys.

Tue Apr 07 00:00:00 UTC 2020

NFκB-Induced Periostin Activates Integrin-β3 Signaling to Promote Renal Injury in GN.

Journal of the American Society of Nephrology : JASN

Prakoura N,Kavvadas P,Kormann R,Dussaule JC,Chadjichristos CE,Chatziantoniou C

PA5-34641 was used in Immunohistochemistry-immunofluorescence to study the mechanism of indution and the role of periostin in renal disease.

Mon May 01 00:00:00 UTC 2017

NFκB-Induced Periostin Activates Integrin-β3 Signaling to Promote Renal Injury in GN.

Journal of the American Society of Nephrology : JASN

Prakoura N,Kavvadas P,Kormann R,Dussaule JC,Chadjichristos CE,Chatziantoniou C

PA5-34641 was used in Immunohistochemistry-immunofluorescence to study the mechanism of indution and the role of periostin in renal disease.

Mon May 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: fasciclin I-like; Fasciclin-I like; MGC119510; MGC119511; OSF 2; OSF-2; osteoblast specific factor 2; osteoblast specific factor 2 (fasciclin I-like); Osteoblast-specific factor 2; periodontal ligament-specific periostin; Periostin; periostin, osteoblast specific factor; periostin-like factor; PN; RP11 412K4.1; RP11-412K4.1

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Gene Aliases: A630052E07Rik; AI747096; OSF-2; OSF2; PDLPOSTN; peri; PLF; PN; POSTN

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UniProt ID: (Human) Q15063, (Mouse) Q62009

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Entrez Gene ID: (Human) 10631, (Mouse) 50706

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Function(s)

protein binding heparin binding metal ion binding cell adhesion molecule binding

Process(es)

skeletal system development response to hypoxia negative regulation of cell-matrix adhesion regulation of systemic arterial blood pressure cell adhesion regulation of Notch signaling pathway response to mechanical stimulus response to muscle activity positive regulation of smooth muscle cell migration extracellular matrix organization response to estradiol cellular response to fibroblast growth factor stimulus cellular response to vitamin K cellular response to tumor necrosis factor cellular response to transforming growth factor beta stimulus negative regulation of substrate adhesion-dependent cell spreading positive regulation of chemokine (C-C motif) ligand 2 secretion neuron projection extension bone regeneration tissue development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Periostin Polyclonal Antibody

Periostin Antibody (PA5-34641) in ICC/IF

Product Details

PA5-34641

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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