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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Perlecan Antibodies

          Invitrogen

          Perlecan Monoclonal Antibody (7B5)

          10 Published Figures
          25 References
          View all (16) Perlecan antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite Perlecan Monoclonal Antibody (7B5)

          • Antibody Testing Data (4)
          • Published Figures (10)
          Perlecan Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Perlecan Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          Perlecan Antibody (13-4400) in ICC/IF

          Immunofluorescence analysis of HSPG2 was performed using 70% confluent log phase HUVEC and SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Perlecan Monoclonal Antibody (7B5) (Product # 13-4400, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Produ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Perlecan Antibody in Immunocytochemistry (ICC/IF)
          Perlecan Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Perlecan Antibody in Immunohistochemistry (IHC)
          Perlecan Antibody in Western Blot (WB)
          Perlecan Antibody in Immunohistochemistry (IHC)
          Perlecan Antibody in Immunohistochemistry (IHC)
          Perlecan Antibody in Immunohistochemistry (IHC)
          Perlecan Antibody in Western Blot (WB)
          Perlecan Antibody in Western Blot (WB)
          Perlecan Antibody in Western Blot (WB)
          Perlecan Antibody in ELISA (ELISA)
          Perlecan Antibody in Immunomicroscopy (IM)
          Perlecan Antibody in Immunomicroscopy (IM)
          Perlecan Antibody in Immunomicroscopy (IM)

          Product Details

          13-4400

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500
          View 6 publications 6 publications

          Immunohistochemistry (IHC)

          -
          View 9 publications 9 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:10-1:50
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:100
          View 4 publications 4 publications

          ELISA (ELISA)

          Assay-dependent
          View 2 publications 2 publications

          Immunomicroscopy (IM)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse, Non-human primate

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          7B5

          Immunogen

          rec-Perlecan domain III.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          purified

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533020

          Target Information

          Perlecan is a major heparan-sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth factors, perlecan has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); HSPG; Perlecan; perlecan proteoglycan; PLC; RP11-132G19.2

          View more View less

          Gene Aliases: HSPG; HSPG2; PLC; PRCAN; SJA; SJS; SJS1

          View more View less

          UniProt ID: (Human) P98160

          View more View less

          Entrez Gene ID: (Human) 3339

          View more View less

          Function(s)
          calcium ion binding protein binding protein C-terminus binding
          Process(es)
          retinoid metabolic process angiogenesis glycosaminoglycan biosynthetic process glycosaminoglycan catabolic process extracellular matrix disassembly extracellular matrix organization glycosaminoglycan metabolic process lipoprotein metabolic process cellular protein metabolic process
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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