Invitrogen

Perlecan Monoclonal Antibody (A7L6)

1 Published Figure

10 References

View all (16) Perlecan antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (1)

Perlecan Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

Perlecan Antibody (MA1-06821) in ICC/IF

Immunofluorescence analysis of HSPG2 was performed using 70% confluent log phase HUVEC and SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Perlecan Monoclonal Antibody (A7L6) (Product # MA1-06821, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Prod... View More

Perlecan Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

MA1-06821

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:25-1:200

Immunocytochemistry (ICC/IF)

1:25-1:200

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Bovine, Fish, Human, Mouse, Rat

Published species

Bovine, Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

A7L6

Immunogen

High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_559394

Product Specific Information

MA1-06821 detects large heparan sulphate proteoglycan in human, mouse, bovine, cod, wolffish, and rat samples.

MA1-06821 has sucessfully been used in Western blotting, immunocytochemistry, immunoprecipitation, and immunohistochemistry procedures. By Western blot it detects a >400 kDa band representing large HSPG.

The MA1-06821 immunogen is a high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.

Target Information

Perlecan is a major heparan-sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth factors, perlecan has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Primary Cilia Direct Murine Articular Cartilage Tidemark Patterning Through Hedgehog Signaling and Ambulatory Load.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Rux D,Helbig K,Han B,Cortese C,Koyama E,Han L,Pacifici M

MA1-06821 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to provide evidence that primary cilia orchestrate postnatal AC morphogenesis including tidemark topography, zonal matrix composition, and ambulation load responses.

Wed Jun 01 00:00:00 UTC 2022

Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition.

Development (Cambridge, England)

Guo D,Ru J,Mao F,Ouyang H,Ju R,Wu K,Liu Y,Liu C

MA1-06821 was used in Immunohistochemistry to highlight a crucial role of Prickle1-mediated polarized basement membrane secretion and deposition in PTD elongation.

Mon Nov 30 00:00:00 UTC 2020

Adipocyte-Derived Versican and Macrophage-Derived Biglycan Control Adipose Tissue Inflammation in Obesity.

Cell reports

Han CY,Kang I,Harten IA,Gebe JA,Chan CK,Omer M,Alonge KM,den Hartigh LJ,Gomes Kjerulf D,Goodspeed L,Subramanian S,Wang S,Kim F,Birk DE,Wight TN,Chait A

Published figure using Perlecan monoclonal antibody (Product # MA1-06821) in Western Blot

Tue Jun 30 00:00:00 UTC 2020

PDGFRβ regulates craniofacial development through homodimers and functional heterodimers with PDGFRα.

Genes & development

Fantauzzo KA,Soriano P

MA106821 was used in immunohistochemistry to reveal that platelet-derived growth factor alpha and beta are coexpressed in the craniofacial mesenchyme of mid-gestation mouse embryos

Tue Nov 01 00:00:00 UTC 2016

Reactivating the extracellular matrix synthesis of sulfated glycosaminoglycans and proteoglycans to improve the human skin aspect and its mechanical properties.

Clinical, cosmetic and investigational dermatology

Chajra H,Auriol D,Joly F,Pagnon A,Rodrigues M,Allart S,Redziniak G,Lefevre F

MA106821 was used in immunohistochemistry - paraffin section to show that a defined cosmetic composition results in the production of sulfated glycosaminoglycans and may have anti-ageing benefits

Tue Sep 29 00:00:00 UTC 2020

Liver aging and pseudocapillarization in a Werner syndrome mouse model.

The journals of gerontology. Series A, Biological sciences and medical sciences

Cogger VC,Svistounov D,Warren A,Zykova S,Melvin RG,Solon-Biet SM,O'Reilly JN,McMahon AC,Ballard JW,De Cabo R,Le Couteur DG,Lebel M

MA1-06821 was used in immunohistochemistry to study the accelerated age-related hepatic changes observed in a murine model of Werner syndrome

Mon Sep 01 00:00:00 UTC 2014

LARGE glycans on dystroglycan function as a tunable matrix scaffold to prevent dystrophy.

Nature

Goddeeris MM,Wu B,Venzke D,Yoshida-Moriguchi T,Saito F,Matsumura K,Moore SA,Campbell KP

MA1-06821 was used in immunohistochemistry to study the role of dystroglycan LARGE glycans in the normal functioning of skeletal muscle

Thu Nov 07 00:00:00 UTC 2013

DOT1L regulates dystrophin expression and is critical for cardiac function.

Genes & development

Nguyen AT,Xiao B,Neppl RL,Kallin EM,Li J,Chen T,Wang DZ,Xiao X,Zhang Y

MA1-06821 was used in immunohistochemistry to study the role of DOT1L in regulating dystrophin expression and cardiac function

Tue Feb 01 00:00:00 UTC 2011

The effect of bovine endosteum-derived particles on the proliferation of human mesenchymal stem cells.

Biomaterials

Nigro J,White JF,Ramshaw JA,Haylock DN,Nilsson SK,Werkmeister JA

MA1-06821 was used in immunohistochemistry to evaluate the usefulness of bovine endosteum-derived particles in stem cell culture

Thu Jul 01 00:00:00 UTC 2010

Synthesis of syndecan-1 by skeletal muscle cells is an early response to infection with Trichinella spiralis but is not essential for nurse cell development.

Infection and immunity

Beiting DP,Park PW,Appleton JA

MA1-06821 was used in immunohistochemistry to study the synthesis of syndecan-1 by skeletal muscle cells in response to Trichinella spiralis infection

Wed Mar 01 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: Basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); HSPG; Perlecan; perlecan proteoglycan; PLC; RP11-132G19.2

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Gene Aliases: AI852380; BOS_2497; HSPG; HSPG2; Pcn; per; PLC; PRCAN; SJA; SJS; SJS1

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UniProt ID: (Human) P98160, (Mouse) Q05793

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Entrez Gene ID: (Human) 3339, (Bovine) 444872, (Mouse) 15530, (Rat) 313641

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Function(s)

calcium ion binding protein binding protein C-terminus binding protease binding metal ion binding

Process(es)

retinoid metabolic process angiogenesis glycosaminoglycan biosynthetic process glycosaminoglycan catabolic process extracellular matrix disassembly extracellular matrix organization glycosaminoglycan metabolic process lipoprotein metabolic process cellular protein metabolic process endochondral ossification chondrocyte differentiation brain development protein localization embryonic skeletal system morphogenesis cardiac muscle tissue development cartilage development involved in endochondral bone morphogenesis

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Perlecan Monoclonal Antibody (A7L6)

Perlecan Antibody (MA1-06821) in ICC/IF

Product Details

MA1-06821

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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