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Invitrogen
Phospho-4EBP1 (Thr37) Recombinant Rabbit Monoclonal Antibody (7D0O2)
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Phospho-4EBP1 (Thr37) Antibody (MA5-55313) in WB
Western blot analysis of lysates from C6 cells, using Phospho-4EBP1 (Thr37) Recombinant Monoclonal Antibody (Product # MA5-55313) at 1:1,000 dilution. C6 cells were treated by IGF-1 (100 ng/mL) at 37&(8)451; for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer:... View More
Product Details
MA5-55313
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7D0O2
Immunogen
A synthetic phosphorylated peptide around T37 of human EIF4EBP1.
Conjugate
Form
Liquid
Concentration
0.64 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667554
Target Information
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E, which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 4E-BP1; eIF4E-binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; MGC431; MGC4316; P/OKCL.6; PHAS-1; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1
Gene Aliases: Eif4ebp1; PHAS-I
UniProt ID: (Rat) Q62622
Entrez Gene ID: (Rat) 116636
G1/S transition of mitotic cell cycle
IRES-dependent translational initiation
response to ischemia
insulin receptor signaling pathway
lung development
negative regulation of protein complex assembly
TOR signaling
response to ethanol
positive regulation of mitotic cell cycle
negative regulation of translational initiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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