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          • Primary Antibodies ›
          • ABL1/ABL2 Antibodies

          Invitrogen

          Phospho-ABL1/ABL2 (Tyr393) Polyclonal Antibody

          View all (6) ABL1/ABL2 antibodies

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          Cite Phospho-ABL1/ABL2 (Tyr393) Polyclonal Antibody

          • Antibody Testing Data (3)
          Phospho-ABL1/ABL2 (Tyr393) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-ABL1/ABL2 (Tyr393) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          Phospho-ABL1/ABL2 (Tyr393) Antibody (PA5-99344) in ICC/IF

          Immunofluorescent analysis of Phospho-c-Abl (Tyr412) in K562 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min, 37°C) incubated with Phospho-c-Abl (Tyr412) polyclonal antibody (Product # PA5-99344) using a dilution of 1:400 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-ABL1/ABL2 (Tyr393) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-ABL1/ABL2 (Tyr393) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-ABL1/ABL2 (Tyr393) Antibody in Western Blot (WB)

          Product Details

          PA5-99344

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Non-human primate

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human ABL1(Accession P00519), corresponding to amino acid residues around phosphorylated Tyr393.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          sequential chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2818277

          Product Specific Information

          Antibody detects endogenous levels of ABL1/2 only when phosphorylated at Tyr393, which site historically referenced as Tyr412.

          Target Information

          The Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). The major translational product of c-Abl has been identified as a protein with tyrosine kinase activity and an SH2 domain. The Abl oncogene is implicated in several human leukemias including 90-95% of chronic myelocytic leukemia (CML), 20-25% of adult acute lymphoblastic leukemia (ALL) and 2-5% of pediatric ALL. In these leukemias the c-Abl proto-oncogene undergoes a (9;22) chromosomal translocation producing the Philadelphia (Ph1) chromosome. The molecular consequence of this translocation is the generation of a chimeric Bcr/c-Abl mRNA encoding activated Abl protein-tyrosine kinase.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Abelson murine leukemia oncogene; Abelson murine leukemia viral oncogene homolog 1; Abelson murine leukemia viral oncogene homolog 2; Abelson tyrosine-protein kinase 1; Abelson tyrosine-protein kinase 2; Abelson-related gene protein; abl2; bcr/c-abl oncogene protein; c-abl oncogene 1, receptor tyrosine kinase; c-abl oncogene 2, non-receptor tyrosine kinase; p150; Proto-oncogene c-Abl; proto-oncogene tyrosine-protein kinase ABL1; tyrosine kinase ARG; Tyrosine-protein kinase ABL1; Tyrosine-protein kinase ABL2; Tyrosine-protein kinase ARG; v-abl Abelson murine leukemia oncogene 1; v-abl Abelson murine leukemia viral oncogene homolog 1; v-abl Abelson murine leukemia viral oncogene homolog 2; v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)

          View more View less

          Gene Aliases: AA536808; ABL; ABL1; ABL2; ABLL; AI325092; ARG; bcr/abl; c-ABL; c-ABL1; E430008G22Rik; JTK7; p150; v-abl

          View more View less

          UniProt ID: (Human) P00519, (Human) P42684, (Mouse) P00520

          View more View less

          Entrez Gene ID: (Human) 25, (Human) 27, (Mouse) 11350, (Mouse) 11352

          View more View less

          Function(s)
          magnesium ion binding DNA binding actin monomer binding nicotinate-nucleotide adenylyltransferase activity protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein kinase C binding receptor binding protein binding ATP binding protein C-terminus binding SH3 domain binding syntaxin binding manganese ion binding actin filament binding mitogen-activated protein kinase binding proline-rich region binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding protein domain specific binding metal ion binding delta-catenin binding
          Process(es)
          B-1 B cell homeostasis positive regulation of protein phosphorylation B cell proliferation involved in immune response transitional one stage B cell differentiation mismatch repair regulation of transcription, DNA-templated cellular protein modification process autophagy cellular response to DNA damage stimulus DNA damage induced protein phosphorylation response to oxidative stress cell cycle arrest mitotic nuclear division epidermal growth factor receptor signaling pathway positive regulation of cytosolic calcium ion concentration intrinsic apoptotic signaling pathway in response to DNA damage regulation of autophagy peptidyl-tyrosine phosphorylation cerebellum morphogenesis negative regulation of cell-cell adhesion microspike assembly actin cytoskeleton organization regulation of endocytosis regulation of cell adhesion negative regulation of BMP signaling pathway regulation of axon extension regulation of microtubule polymerization regulation of actin cytoskeleton organization positive regulation of osteoblast proliferation substrate adhesion-dependent cell spreading cellular response to oxidative stress platelet-derived growth factor receptor-beta signaling pathway peptidyl-tyrosine autophosphorylation Fc-gamma receptor signaling pathway involved in phagocytosis regulation of cell proliferation signal transduction in response to DNA damage positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling innate immune response establishment of protein localization negative regulation of mitotic cell cycle positive regulation of mitotic cell cycle alpha-beta T cell differentiation protein autophosphorylation spleen development thymus development collateral sprouting positive regulation of peptidyl-tyrosine phosphorylation activated T cell proliferation B cell receptor signaling pathway neuromuscular process controlling balance positive regulation of muscle cell differentiation positive regulation of release of sequestered calcium ion into cytosol positive regulation of oxidoreductase activity negative regulation of ubiquitin-protein transferase activity mitochondrial depolarization Bergmann glial cell differentiation cellular response to hydrogen peroxide negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide negative regulation of protein serine/threonine kinase activity actin filament branching positive regulation of interleukin-2 secretion negative regulation of phospholipase C activity positive regulation of neuron death positive regulation of interferon-gamma secretion regulation of extracellular matrix organization cellular response to dopamine positive regulation of microtubule binding positive regulation of actin filament binding activation of protein kinase C activity positive regulation of Wnt signaling pathway, planar cell polarity pathway regulation of cell motility regulation of actin cytoskeleton reorganization negative regulation of endothelial cell apoptotic process negative regulation of cellular senescence regulation of response to DNA damage stimulus cell adhesion signal transduction transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of phospholipase C activity positive regulation of neuron projection development cell migration cellular response to retinoic acid DNA repair protein phosphorylation endocytosis phagocytosis apoptotic process phosphorylation B cell proliferation platelet-derived growth factor receptor signaling pathway regulation of cell cycle regulation of cellular senescence substrate-dependent cell migration, cell extension actin filament organization learning visual learning neuron remodeling positive regulation of protein binding cellular protein localization negative regulation of Rho protein signal transduction exploration behavior dendrite morphogenesis actin filament bundle assembly dendritic spine maintenance
          It has to be done as per old AB suggested Products section.
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