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          • Primary Antibodies ›
          • AKT Pan Antibodies

          Invitrogen

          Phospho-AKT Pan (Thr308, Thr305, Thr309) Recombinant Rabbit Monoclonal Antibody (5E9G5)

          View all (64) AKT Pan antibodies

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          Cite Phospho-AKT Pan (Thr308, Thr305, Thr309) Recombinant Rabbit Monoclonal Antibody (5E9G5)

          • Antibody Testing Data (1)
          Phospho-AKT Pan (Thr308, Thr305, Thr309) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-AKT Pan (Thr308, Thr305, Thr309) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

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          Phospho-AKT Pan (Thr308, Thr305, Thr309) Antibody (MA5-42960) in WB

          Western blot analysis of Phospho-AKT Pan (Thr308, Thr305, Thr309) in extracts of various cell lines. Samples were incubated with Phospho-AKT Pan (Thr308, Thr305, Thr309) Monoclonal antibody (Product # MA5-42960) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-AKT Pan (Thr308, Thr305, Thr309) Antibody in Western Blot (WB)

          Product Details

          MA5-42960

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          5E9G5

          Immunogen

          A synthetic phosphorylated peptide around T308 of human Akt (NP_0051542)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol, 0.05% BSA

          Contains

          0.05% ProClin 300

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2912101

          Product Specific Information

          Positive test controls include: NIH/3T3, C6.

          Immunogen sequence: MKTFC

          Target Information

          AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: AKT1 kinase; Akt1m protein; AKT2 kinase; AKT3 kinase; DKFZp434N0250; kinase Akt1; MGC99656; murine thymoma viral (v-akt) oncogene homolog 1; murine thymoma viral (v-akt) oncogene homolog 2; pan akt; PKB; PKB alpha; PKB beta; PKB gamma; Protein kinase Akt-2; Protein kinase Akt-3; Protein kinase B; Protein kinase B alpha; Protein kinase B beta; Protein kinase B gamma; protein kinase B, beta; protein kinase B, gamma; protein kinase B-alpha; Proto-oncogene c-Akt; RAC protein kinase alpha RAC-PK alpha; RAC protein kinase beta RAC-PK beta; RAC-alpha serine/threonine-protein kinase; RAC-beta serine/threonine protein kinase; RAC-beta serine/threonine-protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; thymoma viral proto-oncogene 2; thymoma viral proto-oncogene 3; v-akt murine thymoma viral oncogene 3; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)

          View more View less

          Gene Aliases: 2410016A19Rik; AI851531; Akt; Akt1; Akt2; Akt3; AW554154; D930002M15Rik; Nmf350; PKB; PKB/Akt; PKBalpha; PKBbeta; Pkbg; Rac

          View more View less

          UniProt ID: (Mouse) P31750, (Rat) P47196, (Rat) P47197, (Mouse) Q60823, (Rat) Q63484, (Mouse) Q9WUA6

          View more View less

          Entrez Gene ID: (Mouse) 11651, (Rat) 24185, (Rat) 25233, (Mouse) 11652, (Rat) 29414, (Mouse) 23797

          View more View less

          Function(s)
          nucleotide binding protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups enzyme binding protein kinase binding nitric-oxide synthase regulator activity identical protein binding phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding
          Process(es)
          positive regulation of protein phosphorylation glycogen biosynthetic process glucose metabolic process regulation of translation protein phosphorylation response to osmotic stress multicellular organism development insulin receptor signaling pathway carbohydrate transport positive regulation of signal transduction positive regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport positive regulation of sodium ion transport positive regulation of glucose metabolic process response to muscle activity positive regulation of cell migration positive regulation of vesicle fusion response to nutrient levels positive regulation of fatty acid beta-oxidation peripheral nervous system myelin maintenance response to insulin cellular response to insulin stimulus cellular response to hormone stimulus negative regulation of RNA splicing positive regulation of peptidyl-serine phosphorylation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling positive regulation of nitric oxide biosynthetic process positive regulation of glycogen biosynthetic process positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of positive chemotaxis intracellular protein transmembrane transport cellular response to organic cyclic compound protein localization to plasma membrane positive regulation of protein targeting to membrane positive regulation of cell motility positive regulation of glucose import in response to insulin stimulus carbohydrate metabolic process glycogen metabolic process transport apoptotic process phosphorylation activation of GTPase activity protein import into nucleus, translocation osteoblast differentiation maternal placenta development positive regulation of endothelial cell proliferation regulation of glycogen biosynthetic process translation negative regulation of protein kinase activity activation-induced cell death of T cells inflammatory response cytoskeleton organization signal transduction germ cell development nervous system development aging apoptotic mitochondrial changes response to hormone negative regulation of autophagy positive regulation of fibroblast migration negative regulation of endopeptidase activity regulation of neuron projection development glucose transport protein ubiquitination peptidyl-serine phosphorylation cell projection organization protein catabolic process positive regulation of cell growth regulation of cell migration regulation of myelination positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle lipopolysaccharide-mediated signaling pathway negative regulation of fatty acid beta-oxidation response to food positive regulation of cellular protein metabolic process positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of protein localization response to fluid shear stress intracellular signal transduction cellular response to vascular endothelial growth factor stimulus cellular response to decreased oxygen levels glucose homeostasis anagen positive regulation of apoptotic process positive regulation of blood vessel endothelial cell migration positive regulation of fat cell differentiation negative regulation of cell size negative regulation of proteolysis positive regulation of vasoconstriction negative regulation of JNK cascade positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development response to UV-A cellular response to mechanical stimulus cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of establishment of protein localization to plasma membrane negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis cellular response to peptide mitochondrial genome maintenance response to oxidative stress hyaluronan metabolic process response to growth hormone response to insulin-like growth factor stimulus peptidyl-threonine phosphorylation
          It has to be done as per old AB suggested Products section.
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