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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • AKT1 Antibodies

          Invitrogen

          Phospho-AKT1 (Ser473) Polyclonal Antibody

          Advanced Verification
          4 Published Figures
          6 References
          View all (186) AKT1 antibodies

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          Cite Phospho-AKT1 (Ser473) Polyclonal Antibody

          • Antibody Testing Data (1)
          • Published Figures (4)
          • Advanced Verification (2)
          Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
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          Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
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          FIGURE: 1 / 7

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          Phospho-AKT1 (Ser473) Antibody (44-623G) in WB

          Western blot analysis of extracts of NIH3T3 cells using rabbit anti-Akt [pS473] antibody (Product # 44-623G). NIH3T3 cells untreated (lane 1) or treated with PDGF (50 ng/mL, 15 min) (lanes 2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Prior to incubation with the primary antibody, the membrane was incubated with no peptide (lane 1 and 2), the non-phosphorylated peptide ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
          Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
          Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
          Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
          Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
          Phospho-AKT1 (Ser473) Antibody
          Phospho-AKT1 (Ser473) Antibody

          Product Details

          44-623G

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 6 publications 6 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Akt1 that contains serine 473.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Antigen affinity chromatography

          Storage buffer

          Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533700

          Target Information

          AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: AKT1m; C-AKT; CAKT; PKB; PKB alpha; PKB beta; PKB gamma; PKBG; Protein kinase B; Protein kinase B alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1

          View more View less

          Gene Aliases: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA

          View more View less

          UniProt ID: (Human) P31749

          View more View less

          Entrez Gene ID: (Human) 207

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding nitric-oxide synthase regulator activity GTPase activating protein binding identical protein binding phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding
          Process(es)
          protein import into nucleus, translocation osteoblast differentiation maternal placenta development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation glycogen biosynthetic process regulation of glycogen biosynthetic process glucose metabolic process translation regulation of translation cellular protein modification process protein phosphorylation negative regulation of protein kinase activity nitric oxide biosynthetic process activation-induced cell death of T cells inflammatory response cellular response to DNA damage stimulus response to oxidative stress signal transduction G-protein coupled receptor signaling pathway germ cell development aging cell proliferation insulin receptor signaling pathway apoptotic mitochondrial changes response to heat negative regulation of autophagy negative regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of glucose metabolic process negative regulation of endopeptidase activity regulation of neuron projection development regulation of phosphatidylinositol 3-kinase signaling glucose transport phosphorylation protein ubiquitination peptidyl-serine phosphorylation peptidyl-threonine phosphorylation spinal cord development cell projection organization cell differentiation protein catabolic process platelet activation hyaluronan metabolic process positive regulation of cell growth regulation of cell migration endocrine pancreas development T cell costimulation regulation of myelination positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle lipopolysaccharide-mediated signaling pathway negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity response to food positive regulation of cellular protein metabolic process peripheral nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation response to fluid shear stress intracellular signal transduction cellular response to vascular endothelial growth factor stimulus ERBB2 signaling pathway glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of mRNA stability protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of glycogen biosynthetic process positive regulation of epidermal growth factor receptor signaling pathway negative regulation of cell size negative regulation of proteolysis positive regulation of vasoconstriction positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import negative regulation of JNK cascade protein autophosphorylation positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling regulation of nitric-oxide synthase activity positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation response to growth hormone mammary gland epithelial cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development response to UV-A cellular response to mechanical stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus cellular response to organic cyclic compound cellular response to hypoxia establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion positive regulation of establishment of protein localization to plasma membrane negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis chemical synaptic transmission, postsynaptic negative regulation of protein kinase activity by protein phosphorylation positive regulation of protein localization to nucleus negative regulation of neuron death regulation of signal transduction by p53 class mediator regulation of cell cycle checkpoint negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
          It has to be done as per old AB suggested Products section.
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