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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • AKT1 Antibodies

          Invitrogen

          Phospho-AKT1 (Ser473) Polyclonal Antibody

          1 Reference
          View all (186) AKT1 antibodies

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          Datasheet
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          Datasheet
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          Cite Phospho-AKT1 (Ser473) Polyclonal Antibody

          • Antibody Testing Data (8)
          Phospho-AKT1 (Ser473) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-AKT1 (Ser473) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          Phospho-AKT1 (Ser473) Antibody (PA5-85513) in ICC/IF

          Immunocytochemistry-Immunofluorescence analysis of Phospho-AKT1-(Ser473) was performed in NIH3T3 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Phospho-AKT1 (Ser473) Polyclonal Antibody (Product # PA5-85513) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-AKT1 (Ser473) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
          Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
          Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
          Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
          Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
          Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)
          Phospho-AKT1 (Ser473) Antibody in Immunoprecipitation (IP)

          Product Details

          PA5-85513

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -

          Immunoprecipitation (IP)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Pig, Rat

          Published species

          Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Carrier-protein conjugated synthetic peptide surrounding phospho Ser473 of human AKT. The exact sequence is proprietary.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.0 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 20% glycerol, 1% BSA

          Contains

          0.025% ProClin 300

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2792653

          Product Specific Information

          Keep as concentrated solution.

          Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Cat (100%), Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%).

          Positive Control: PC-12, Rat2, MCF-7, MCF-7(500 nM PMA treatment for 1 hr), MCF-7(500 nM PMA treatment for 2 hr), MCF-7(500 nM PMA treatment for 4 hr), MCF-7(500 nM PMA treatment for 6 hr), HUVEC, HUVEC (5 ng/mL VEGF treatment for 10 min), NIH-3T3, NIH-3T3 (starvation for 16 hr and 5 ng/mL PDGF treatment for 15 min).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: AKT1 kinase; AKT1m; Akt1m protein; C-AKT; CAKT; murine thymoma viral (v-akt) oncogene homolog 1; PKB; PKB alpha; PKB beta; PKB gamma; PKBG; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; serine-threonine protein kinase; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like 1; v-akt murine thymoma viral oncogene-like protein 1

          View more View less

          Gene Aliases: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA

          View more View less

          UniProt ID: (Human) P31749, (Mouse) P31750, (Rat) P47196

          View more View less

          Entrez Gene ID: (Human) 207, (Pig) 100126861, (Mouse) 11651, (Rat) 24185

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding nitric-oxide synthase regulator activity GTPase activating protein binding identical protein binding phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding
          Process(es)
          protein import into nucleus, translocation osteoblast differentiation maternal placenta development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation carbohydrate metabolic process glycogen metabolic process glycogen biosynthetic process regulation of glycogen biosynthetic process glucose metabolic process translation regulation of translation protein phosphorylation negative regulation of protein kinase activity transport apoptotic process activation-induced cell death of T cells inflammatory response cytoskeleton organization signal transduction multicellular organism development germ cell development nervous system development aging insulin receptor signaling pathway apoptotic mitochondrial changes carbohydrate transport response to hormone negative regulation of autophagy negative regulation of plasma membrane long-chain fatty acid transport positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of glucose metabolic process negative regulation of endopeptidase activity regulation of neuron projection development glucose transport phosphorylation protein ubiquitination peptidyl-serine phosphorylation cell projection organization protein catabolic process positive regulation of cell growth regulation of cell migration regulation of myelination positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle lipopolysaccharide-mediated signaling pathway negative regulation of fatty acid beta-oxidation response to food positive regulation of cellular protein metabolic process peripheral nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus regulation of protein localization positive regulation of peptidyl-serine phosphorylation response to fluid shear stress intracellular signal transduction cellular response to vascular endothelial growth factor stimulus cellular response to decreased oxygen levels glucose homeostasis anagen positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of glycogen biosynthetic process negative regulation of cell size negative regulation of proteolysis positive regulation of vasoconstriction positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import negative regulation of JNK cascade positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development response to UV-A cellular response to mechanical stimulus cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus cellular response to organic cyclic compound cellular response to hypoxia positive regulation of establishment of protein localization to plasma membrane negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis cellular response to peptide response to oxidative stress hyaluronan metabolic process response to growth hormone response to insulin-like growth factor stimulus cellular protein modification process nitric oxide biosynthetic process cellular response to DNA damage stimulus G-protein coupled receptor signaling pathway cell proliferation response to heat negative regulation of gene expression regulation of phosphatidylinositol 3-kinase signaling peptidyl-threonine phosphorylation spinal cord development cell differentiation platelet activation endocrine pancreas development T cell costimulation positive regulation of endodeoxyribonuclease activity ERBB2 signaling pathway regulation of mRNA stability positive regulation of epidermal growth factor receptor signaling pathway protein autophosphorylation phosphatidylinositol-mediated signaling regulation of nitric-oxide synthase activity mammary gland epithelial cell differentiation establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion chemical synaptic transmission, postsynaptic negative regulation of protein kinase activity by protein phosphorylation positive regulation of protein localization to nucleus negative regulation of neuron death regulation of signal transduction by p53 class mediator regulation of cell cycle checkpoint negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway cellular response to nerve growth factor stimulus negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
          It has to be done as per old AB suggested Products section.
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