Invitrogen

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody

View all (16) AMPK alpha-1,2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-110151

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
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Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A phospho specific peptide corresponding to residues surrounding T172 of human AMPK alpha.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.79 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2855562

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha 2; AMPK alpha-1 chain; AMPK alpha-2 chain; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK-alpha-1 chain; AMPK-alpha-2 chain; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; pAMPK; prkaa2; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; Tau-protein kinase PRKAA1

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Gene Aliases: 2310008I11Rik; A830082D05; AI194361; AI450832; AL024255; AMPK; AMPK1; AMPK2; AMPKa1; AMPKa2; AMPKalpha1; AMPKalpha2; C130083N04Rik; PRKAA; PRKAA1; PRKAA2

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UniProt ID: (Human) Q13131, (Human) P54646, (Rat) P54645, (Mouse) Q5EG47, (Rat) Q09137, (Mouse) Q8BRK8

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Entrez Gene ID: (Human) 5562, (Human) 5563, (Rat) 65248, (Mouse) 105787, (Rat) 78975, (Mouse) 108079

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Function(s)
chromatin binding protein kinase activity AMP-activated protein kinase activity cAMP-dependent protein kinase activity protein binding ATP binding protein C-terminus binding kinase binding histone serine kinase activity metal ion binding [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity tau-protein kinase activity [acetyl-CoA carboxylase] kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity eukaryotic elongation factor-2 kinase activator activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein binding, bridging
Process(es)
transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation fatty acid biosynthetic process cholesterol biosynthetic process carnitine shuttle response to stress cell cycle arrest signal transduction lipid biosynthetic process positive regulation of autophagy response to muscle activity Wnt signaling pathway macroautophagy positive regulation of macroautophagy regulation of macroautophagy cellular response to nutrient levels negative regulation of TOR signaling histone-serine phosphorylation intracellular signal transduction cellular response to drug cellular response to glucose starvation regulation of fatty acid biosynthetic process glucose homeostasis regulation of circadian rhythm negative regulation of apoptotic process positive regulation of glycolytic process rhythmic process fatty acid homeostasis cellular response to prostaglandin E stimulus regulation of signal transduction by p53 class mediator regulation of energy homeostasis activation of MAPK activity glucose metabolic process autophagy positive regulation of cell proliferation response to UV cold acclimation response to gamma radiation positive regulation of gene expression response to activity negative regulation of translation fatty acid oxidation response to caffeine response to hydrogen peroxide positive regulation of gluconeogenesis positive regulation of fatty acid oxidation positive regulation of glucose import negative regulation of lipid catabolic process protein heterooligomerization regulation of vesicle-mediated transport cellular response to hydrogen peroxide cellular response to ethanol cellular response to organonitrogen compound cellular response to hypoxia response to camptothecin negative regulation of glucose import in response to insulin stimulus regulation of lipid metabolic process lipid metabolic process fatty acid metabolic process steroid biosynthetic process steroid metabolic process cholesterol metabolic process sterol biosynthetic process phosphorylation chromatin modification regulation of peptidyl-serine phosphorylation positive regulation of skeletal muscle tissue development regulation of gene expression response to hypoxia positive regulation of cholesterol biosynthetic process negative regulation of glucosylceramide biosynthetic process
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