Invitrogen

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)

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Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody (PA5-17831) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), A-431 treated with Metformin (10mM for 24 h) (Lane 2), Hep G2 (Lane 3), Hep G2 treated with Metformin (10mM for 24 h) (Lane 4), U-87 MG (Lane 5), and U-87 MG treated with AICAR (1mM for 48 h) (Lane 6). The blot was probed with Phospho-AMPK alpha-1,2 (Thr183, Thr172) Monoclonal Antibody (Product # PA5-17831, 1:1000 d... View More

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody

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Product Details

PA5-17831

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 14 publications 14 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Pig, Plant, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptides corresponding to residues surrounding Thr183 of human AMPKa 1 and Thr172 of AMPKa 2

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

83 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/mL BSA

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10983715

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

WIP1 is a novel specific target for growth hormone action.

iScience

Apaydin T,Zonis S,Zhou C,Valencia CW,Barrett R,Strous GJ,Mol JA,Chesnokova V,Melmed S

PA5-17831 was used in Western Blot to identify WIP1 as a specific target for GH-mediated epithelial DNA damage accumulation.

Fri Nov 17 00:00:00 UTC 2023

Ischemia reperfusion myocardium injuries in type 2 diabetic rats: Effects of ketamine and insulin on LC3-II and mTOR expression.

International journal of immunopathology and pharmacology

Tao Z,Lin R,Zhang R,He P,Lei C,Li Y

PA5-17831 was used in Western Blotting to suggest that ketamine combined with insulin administration remarkably protects I/R-induced myocardial injury in rats with T2DM by reducing the dysregulation of autophagy.

Thu Nov 02 00:00:00 UTC 2023

Mitochondria as Target for Tumor Management of Hemangioendothelioma.

Antioxidants & redox signaling

Gordillo GM,Biswas A,Singh K,Sen A,Guda PR,Miller C,Pan X,Khanna S,Cadenas E,Sen CK

PA5-17831 was used in Western Blotting to observe that safe natural products may target tumor cell mitochondria and sharply lower radiation dosage required for tumor management warrants clinical testing.

Sun Jan 10 00:00:00 UTC 2021

The combination of lactoferrin and linolenic acid inhibits colorectal tumor growth through activating AMPK/JNK-related apoptosis pathway.

PeerJ

Yao Q,Li H,Fan L,Huang S,Wang J,Zheng N

PA5-17831 was used in Western Blot to investigate if lactoferrin + linolenic acid combination inhibites HT29 tumor formation by activating the AMPK/JNK related pathway.

Sun Aug 11 00:00:00 UTC 2024

The combination of lactoferrin and linolenic acid inhibits colorectal tumor growth through activating AMPK/JNK-related apoptosis pathway.

PeerJ

Yao Q,Li H,Fan L,Huang S,Wang J,Zheng N

PA5-17831 was used in Western Blot to investigate if lactoferrin + linolenic acid combination inhibites HT29 tumor formation by activating the AMPK/JNK related pathway.

Sun Aug 11 00:00:00 UTC 2024

SIRT6 enhances oxidative phosphorylation in breast cancer and promotes mammary tumorigenesis in mice.

Cancer & metabolism

Becherini P,Caffa I,Piacente F,Damonte P,Vellone VG,Passalacqua M,Benzi A,Bonfiglio T,Reverberi D,Khalifa A,Ghanem M,Guijarro A,Tagliafico L,Sucameli M,Persia A,Monacelli F,Cea M,Bruzzone S,Ravera S,Nencioni A

PA5-17831 was used in Western Blotting to determine the effects of the enzymatic activity of SIRT6.

Fri Jan 22 00:00:00 UTC 2021

SIRT6 enhances oxidative phosphorylation in breast cancer and promotes mammary tumorigenesis in mice.

Cancer & metabolism

PA5-17831 was used in Western Blotting to determine the effects of the enzymatic activity of SIRT6.

Fri Jan 22 00:00:00 UTC 2021

CTRP13 Protects H9c2 Cells Against Hypoxia/Reoxygenation (H/R)-Induced Injury Via Regulating the AMPK/Nrf2/ARE Signaling Pathway.

Cell transplantation

Jiang W,Song J,Zhang S,Ye Y,Wang J,Zhang Y

PA5-17831 was used in Western Blotting to study the functional role of C1q/TNF-related protein 13 (CTRP13) in H9c2 cells exposed to hypoxia/reoxygenation.

Tue Feb 01 00:00:00 UTC 2022

Fasting-mimicking diet and hormone therapy induce breast cancer regression.

Nature

Caffa I,Spagnolo V,Vernieri C,Valdemarin F,Becherini P,Wei M,Brandhorst S,Zucal C,Driehuis E,Ferrando L,Piacente F,Tagliafico A,Cilli M,Mastracci L,Vellone VG,Piazza S,Cremonini AL,Gradaschi R,Mantero C,Passalacqua M,Ballestrero A,Zoppoli G,Cea M,Arrighi A,Odetti P,Monacelli F,Salvadori G,Cortellino S,Clevers H,De Braud F,Sukkar SG,Provenzani A,Longo VD,Nencioni A

PA5-17831 was used in Western Blotting to support further clinical studies of a fasting-mimicking diet as an adjuvant to oestrogen therapy in hormone-receptor-positive breast cancer.

Wed Jul 01 00:00:00 UTC 2020

Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways.

International journal of molecular sciences

Kurowska P,Mlyczyńska E,Estienne A,Barbe A,Rajska I,Soból K,Poniedziałek-Kempny K,Dupont J,Rak A

PA5-17831 was used in Western Blotting to show that vaspin, by improving in vitro oocyte maturation via MAP3/1 and PRKAA1 kinase pathways, can be a new factor to improve in vitro fertilisation protocols.

Tue Dec 08 00:00:00 UTC 2020

Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways.

International journal of molecular sciences

Kurowska P,Mlyczyńska E,Estienne A,Barbe A,Rajska I,Soból K,Poniedziałek-Kempny K,Dupont J,Rak A

Tue Dec 08 00:00:00 UTC 2020

C‑type natriuretic peptide prevents angiotensin II‑induced atrial connexin 40 and 43 dysregulation by activating AMP‑activated kinase signaling.

Molecular medicine reports

Ding DZ,Jia YN,Zhang B,Guan CM,Zhou S,Li X,Cui X

PA5-17831 was used in Western Blotting to show that C-type natriuretic peptide (CNP) may be therapeutically useful for clinical conditions involving cardiac dysregulation of Cx expression-related diseases.

Sun Dec 01 00:00:00 UTC 2019

Cryptotanshinone from the Salvia miltiorrhiza Bunge Attenuates Ethanol-Induced Liver Injury by Activation of AMPK/SIRT1 and Nrf2 Signaling Pathways.

International journal of molecular sciences

Nagappan A,Kim JH,Jung DY,Jung MH

Published figure using Phospho-AMPK alpha-1,2 (Thr183, Thr172) polyclonal antibody (Product # PA5-17831) in Western Blot

Mon Dec 30 00:00:00 UTC 2019

Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells.

Cells

Saladini S,Aventaggiato M,Barreca F,Morgante E,Sansone L,Russo MA,Tafani M

Published figure using Phospho-AMPK alpha-1,2 (Thr183, Thr172) polyclonal antibody (Product # PA5-17831) in Western Blot

Mon Jan 14 00:00:00 UTC 2019

Trehalose 6-Phosphate Positively Regulates Fatty Acid Synthesis by Stabilizing WRINKLED1.

The Plant cell

Zhai Z,Keereetaweep J,Liu H,Feil R,Lunn JE,Shanklin J

PA5-17831 was used in Western Blotting to indicate that the trehalose 6-phosphate (T6P) sensitivity of SnRK1 in vivo is GRIK1/GRIK2 dependent.

Mon Oct 01 00:00:00 UTC 2018

Gomisin N Alleviates Ethanol-Induced Liver Injury through Ameliorating Lipid Metabolism and Oxidative Stress.

International journal of molecular sciences

Nagappan A,Jung DY,Kim JH,Lee H,Jung MH

Published figure using Phospho-AMPK alpha-1,2 (Thr183, Thr172) polyclonal antibody (Product # PA5-17831) in Western Blot

Sat Sep 01 00:00:00 UTC 2018

Palmitoleic acid reduces intramuscular lipid and restores insulin sensitivity in obese sheep.

Diabetes, metabolic syndrome and obesity : targets and therapy

Duckett SK,Volpi-Lagreca G,Alende M,Long NM

PA5-17831 was used in western blot to investigate the effects of palmitoleic acid on lipogenesis and circulating insulin levels in obese sheep

Thu Nov 27 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha 2; AMPK alpha-1 chain; AMPK alpha-2 chain; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK-alpha-1 chain; AMPK-alpha-2 chain; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; pAMPK; prkaa2; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; Tau-protein kinase PRKAA1

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Gene Aliases: 2310008I11Rik; A830082D05; AI194361; AI450832; AL024255; AMPK; AMPK1; AMPK2; AMPKa1; AMPKa2; AMPKalpha1; AMPKalpha2; C130083N04Rik; PRKAA; PRKAA1; PRKAA2

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UniProt ID: (Human) Q13131, (Human) P54646, (Rat) P54645, (Mouse) Q5EG47, (Rat) Q09137, (Mouse) Q8BRK8

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Entrez Gene ID: (Human) 5562, (Human) 5563, (Rat) 65248, (Mouse) 105787, (Rat) 78975, (Mouse) 108079

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Function(s)

chromatin binding protein kinase activity AMP-activated protein kinase activity cAMP-dependent protein kinase activity protein binding ATP binding protein C-terminus binding kinase binding histone serine kinase activity metal ion binding [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity tau-protein kinase activity [acetyl-CoA carboxylase] kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity eukaryotic elongation factor-2 kinase activator activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein binding, bridging

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody (PA5-17831) in WB

Product Details

PA5-17831

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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