Invitrogen

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Recombinant Superclonal™ Antibody (10HCLC)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

710099

Applications
Tested Dilution
Publications

Western Blot (WB)

2 µg/mL
-

Immunocytochemistry (ICC/IF)

2-4 µg/mL
-
Product Specifications

Species Reactivity

Human, Non-human primate

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

10HCLC

Immunogen

Peptide corresponding to amino acids 168-178 of human AMPK alpha
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532562

Product Specific Information

This antibody is predicted to react with mouse, rat, non-human primate and Drosophila based on sequence homology.

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha 2; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK-alpha-1 chain; AMPK-alpha-2 chain; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; pAMPK; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; Tau-protein kinase PRKAA1

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Gene Aliases: AMPK; AMPK1; AMPK2; AMPKa1; AMPKa2; PRKAA; PRKAA1; PRKAA2

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UniProt ID: (Human) Q13131, (Human) P54646

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Entrez Gene ID: (Human) 5562, (Human) 5563

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Function(s)
chromatin binding protein kinase activity AMP-activated protein kinase activity cAMP-dependent protein kinase activity protein binding ATP binding protein C-terminus binding kinase binding histone serine kinase activity metal ion binding [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity tau-protein kinase activity [acetyl-CoA carboxylase] kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity
Process(es)
transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation fatty acid biosynthetic process cholesterol biosynthetic process carnitine shuttle response to stress cell cycle arrest signal transduction lipid biosynthetic process positive regulation of autophagy response to muscle activity Wnt signaling pathway macroautophagy positive regulation of macroautophagy regulation of macroautophagy cellular response to nutrient levels negative regulation of TOR signaling histone-serine phosphorylation intracellular signal transduction cellular response to drug cellular response to glucose starvation regulation of fatty acid biosynthetic process glucose homeostasis regulation of circadian rhythm negative regulation of apoptotic process positive regulation of glycolytic process rhythmic process fatty acid homeostasis cellular response to prostaglandin E stimulus regulation of signal transduction by p53 class mediator regulation of energy homeostasis activation of MAPK activity response to hypoxia glucose metabolic process positive regulation of cell proliferation response to UV cold acclimation response to gamma radiation positive regulation of gene expression response to activity fatty acid oxidation response to caffeine regulation of peptidyl-serine phosphorylation positive regulation of cholesterol biosynthetic process negative regulation of glucosylceramide biosynthetic process positive regulation of skeletal muscle tissue development negative regulation of lipid catabolic process protein heterooligomerization regulation of vesicle-mediated transport cellular response to hydrogen peroxide cellular response to ethanol cellular response to organonitrogen compound cellular response to hypoxia response to camptothecin negative regulation of glucose import in response to insulin stimulus
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