Invitrogen

Phospho-AMPK alpha-2 (Thr172) Recombinant Rabbit Monoclonal Antibody (4F4)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-33216

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
-

Immunocytochemistry (ICC/IF)

1:20-1:200
-

ELISA (ELISA)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4F4

Immunogen

A synthesized peptide derived from human Phospho-PRKAA2 (Thr172)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3 mg/mL

Purification

Protein G, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2812030

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMPK alpha 2; AMPK subunit alpha-2; AMPK-alpha-2 chain; EC 2.7.11.1; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; prkaa2; protein kinase, AMP-activated, alpha 2 catalytic subunit

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Gene Aliases: 2310008I11Rik; A830082D05; AMPK; AMPK2; AMPKa2; AMPKalpha2; PRKAA; PRKAA2

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UniProt ID: (Human) P54646, (Mouse) Q8BRK8

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Entrez Gene ID: (Human) 5563, (Mouse) 108079

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Function(s)
chromatin binding protein kinase activity protein serine/threonine kinase activity AMP-activated protein kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding histone serine kinase activity metal ion binding [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity [acetyl-CoA carboxylase] kinase activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein binding, bridging
Process(es)
transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation fatty acid biosynthetic process cholesterol biosynthetic process carnitine shuttle response to stress cell cycle arrest signal transduction lipid biosynthetic process positive regulation of autophagy response to muscle activity Wnt signaling pathway macroautophagy positive regulation of macroautophagy regulation of macroautophagy cellular response to nutrient levels negative regulation of TOR signaling histone-serine phosphorylation intracellular signal transduction cellular response to drug cellular response to glucose starvation regulation of fatty acid biosynthetic process glucose homeostasis regulation of circadian rhythm negative regulation of apoptotic process positive regulation of glycolytic process rhythmic process fatty acid homeostasis cellular response to prostaglandin E stimulus regulation of signal transduction by p53 class mediator regulation of energy homeostasis lipid metabolic process fatty acid metabolic process steroid biosynthetic process autophagy steroid metabolic process cholesterol metabolic process regulation of gene expression response to activity sterol biosynthetic process phosphorylation chromatin modification regulation of lipid metabolic process response to caffeine protein heterooligomerization
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