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          • Primary Antibodies ›
          • APLF Antibodies

          Invitrogen

          Phospho-APLF (Ser116) Polyclonal Antibody

          View all (8) APLF antibodies

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          Cite Phospho-APLF (Ser116) Polyclonal Antibody

          • Antibody Testing Data (4)
          Phospho-APLF (Ser116) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          Phospho-APLF (Ser116) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          Phospho-APLF (Ser116) Antibody (PA5-105966) in IHC (P)

          Immunohistochemistry analysis of paraffin-embedded Phospho-APLF (Ser116) in human breast tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-APLF (Ser116) polyclonal antibody (Product # PA5-105966) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-APLF (Ser116) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-APLF (Ser116) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-APLF (Ser116) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-APLF (Ser116) Antibody in Western Blot (WB)

          Product Details

          PA5-105966

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:3,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human APLF(Accession Q8IW19), corresponding to amino acid residues around phosphorylated Ser116.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          sequential chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice

          RRID

          AB_2817365

          Product Specific Information

          Antibody detects endogenous levels of APLF only when phosphorylated at Ser116.

          Target Information

          C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks.[supplied by OMIM, Mar 2008].

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Aprataxin and PNK-like factor; aprataxin- and PNK-like factor; Apurinic-apyrimidinic endonuclease APLF; PNK and APTX-like FHA domain-containing protein; PNK and APTX-like FHA protein; XRCC1-interacting protein 1; zinc finger, CX5CX6HX5H motif containing 1

          View more View less

          Gene Aliases: 2010301N04Rik; AI452191; APFL; APLF; C2orf13; PALF; RGD1565557; XIP1; ZCCHH1

          View more View less

          UniProt ID: (Human) Q8IW19, (Mouse) Q9D842

          View more View less

          Entrez Gene ID: (Human) 200558, (Rat) 500247, (Mouse) 72103

          View more View less

          Function(s)
          nucleotide binding DNA-(apurinic or apyrimidinic site) lyase activity endodeoxyribonuclease activity protein binding 3'-5' exonuclease activity metal ion binding molecular_function lyase activity
          Process(es)
          single strand break repair DNA catabolic process, endonucleolytic double-strand break repair cellular response to DNA damage stimulus biological_process regulation of isotype switching positive regulation of DNA ligation nucleic acid phosphodiester bond hydrolysis DNA repair
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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