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Phospho-ASK1 (Ser83) Antibody (PA5-117169) in WB
Western blot analysis of Phospho-ASK1 (Ser83) in extracts of various cell lines. Samples were incubated with Phospho-ASK1 (Ser83) Polyclonal antibody (Product # PA5-117169) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. K562 cell lysate were treated by CIP (20 µL CIP for each 400 µL cell lysate) at 37°C for 1 hour, treated by TNF-alpha (... View More
Product Details
PA5-117169
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around S83 of human MAP3K5 (NP_0059141)
Conjugate
Form
Liquid
Concentration
0.07 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901799
Product Specific Information
Positive Samples: K-562
Immunogen sequence: GSSVG
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase that is abundantly expressed in human heart and pancreas and are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activates c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death and a catalytically inactive form of ASK1 inhibited TNF-a-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apoptosis signal regulating kinase 1; Apoptosis signal-regulating kinase 1; ASK 1; ASK-1; EC 2.7.11.25; kinase ASK1; M3K5; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; Mitogen-activated protein kinase kinase kinase 5; RP3-325F22.4
Gene Aliases: ASK1; MAP3K5; MAPKKK5; MEKK5
UniProt ID: (Human) Q99683
Entrez Gene ID: (Human) 4217
MAPK cascade
activation of MAPKK activity
response to ischemia
protein phosphorylation
JNK cascade
activation of JUN kinase activity
intrinsic apoptotic signaling pathway in response to oxidative stress
viral process
response to endoplasmic reticulum stress
p38MAPK cascade
positive regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of JUN kinase activity
innate immune response
positive regulation of myoblast differentiation
positive regulation of JNK cascade
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hydrogen peroxide
apoptotic signaling pathway
programmed necrotic cell death
positive regulation of neuron death
cellular response to reactive nitrogen species
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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