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Invitrogen
Phospho-ATF2 (Thr71) Recombinant Rabbit Monoclonal Antibody (10L9M2)
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FIGURE: 1 / 1
Phospho-ATF2 (Thr71) Antibody (MA5-38233) in WB
Western blot analysis of Phospho-ATF2 (Thr71) in extracts of NIH/3T3 cells. Samples were incubated with Phospho-ATF2 (Thr71) Monoclonal antibody (Product # MA5-38233) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 cells were treated by Anisomycin (25 µg/mL) at 37°C for 30 minutes. Lysates/proteins: 25 µg per lane. Blocking buffer... View More
Product Details
MA5-38233
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
10L9M2
Immunogen
A synthetic phosphorylated peptide around T71 of human ATF2 (P15336)
Conjugate
Form
Liquid
Concentration
0.52 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2898149
Product Specific Information
Positive Samples: NIH/3T3
Immunogen sequence: TPTPT
Target Information
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating 2; Activating transcription factor 2; cAMP response element-binding protein CRE-BP1; cAMP-dependent transcription factor ATF-2; Cyclic AMP-dependent transcription factor ATF-2; MGC111558; MXBP protein; OTTHUMP00000205345; OTTHUMP00000205347; OTTHUMP00000205348; OTTHUMP00000205350; OTTHUMP00000205352; OTTHUMP00000205353; OTTHUMP00000205354
Gene Aliases: Atf-2; Atf2; CRE-BP; Creb2; D130078H02Rik; D18875; mXBP; Tg(Gzma-Klra1)7Wum
UniProt ID: (Mouse) P16951
Entrez Gene ID: (Mouse) 11909
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
enhancer sequence-specific DNA binding
nucleic acid binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
histone acetyltransferase activity
protein binding
cAMP response element binding protein binding
transferase activity
protein kinase binding
cAMP response element binding
sequence-specific DNA binding
metal ion binding
protein heterodimerization activity
outflow tract morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
response to osmotic stress
cellular response to DNA damage stimulus
histone acetylation
intra-S DNA damage checkpoint
positive regulation of transforming growth factor beta2 production
fat cell differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
adipose tissue development
positive regulation of mitochondrial membrane permeability involved in apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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