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Phospho-Arhgap22 (Ser22) Antibody (600-401-J11) in WB
Western Blot of Rabbit anti-ARHGAP22 pS22 antibody. Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1µg/mL for overnight at 4°C. Secondar... View More
Product Details
600-401-J11
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS22 region of ARHGAP22.
Conjugate
Form
Liquid
Concentration
1.07 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-ARHGAP22 pS22 is specific for phosphorylated ARHGAP22 at Serine 22. It also recognizes the S397->A mutation but not the S22->A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100% sequence homology. Cross-reactivity with ARHGAP22 pS22 from other sources has not been determined.
Target Information
GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in down regulation of their active form. ARHGAP22 (Rho GTPase activating protein 22), also known as RHOGAP2, is a 698 amino acid protein that localizes to the cytoplasm, and contains one Rho-GAP domain and one PH domain. ARHGAP22 functions as a GTPase-activating protein for Rac 1 converting it to an inactive GDP-bound state and, through its interaction with ZNF161 (VEZF1), is thought to be useful in transcription regulation. Multiple isoforms of ARHGAP22 exist due to alternative splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: p68RacGAP; RHG22; Rho GTPase-activating protein 22; Rho-type GTPase-activating protein 22
Gene Aliases: ARHGAP22; B230341L19Rik; p68RacGAP; RHOGAP2; RhoGap22
UniProt ID: (Human) Q7Z5H3, (Mouse) Q8BL80
Entrez Gene ID: (Human) 58504, (Mouse) 239027, (Rat) 306279
Performance Guarantee
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