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Rockland
Phospho-Asap1 (Tyr782) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-Asap1 (Tyr782) Antibody (600-401-910) in ICC/IF
Immunofluorescent microscopy using Rocklands Affinity Purified anti-ASAP1 pY782 antibody shows detection of phosphorylated ASAP1 present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.
Product Details
600-401-910
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Chimpanzee,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of mouse ASAP1 protein.
Conjugate
Form
Liquid
Concentration
1.09 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against the phosphorylated form of mouse ASAP1 protein at the pY782 residue. Reactivity occurs against mouse ASAP1 pY782 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated mouse ASAP1 is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with ASAP1 proteins from human, chicken, bovine, dog, rat and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
ASAP1, also known as Development and differentiation enhancing factor 1, is a protein with two ANK repeats, an Arf-GAP domain, a PH domain and a SH3 domain. ASAP1 is a homodimer known to interact with SRC and CRK and its activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2). It possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. It may coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2 and may also function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. It is widely expressed in most of the tissues. ASAP1 is a protooncogene involved in invasive breast cancer and in other types of invasive and malignant tumors, such as uveal melanomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein; 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein; ADP-ribosylation factor-directed GTPase-activating protein 1; ARF GTPase-activating protein 1; Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1; centaurin, beta 4; DEF-1; development and differentiation enhancing factor 1; Development and differentiation-enhancing factor 1; differentiation-enhancing factor 1; PIP2-dependent ARF1 GAP
Gene Aliases: AMAP1; ASAP1; AV239055; BOS_13755; CENTB4; DDEF1; DEF-1; KIAA1249; mKIAA1249; PAG2; PAP; s19; Shag1; ZG14P
UniProt ID: (Bovine) O97902, (Human) Q9ULH1, (Mouse) Q9QWY8, (Rat) Q1AAU6
Entrez Gene ID: (Bovine) 327705, (Dog) 482044, (Human) 50807, (Mouse) 13196, (Rat) 314961
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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