Invitrogen

Phospho-Aurora A/B/C (Thr288, Thr232, Thr198) Polyclonal Antibody

View all (8) Aurora A/B/C antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-117233

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around T288 of human AURKA/AURKB/AURKCAURKA (NP_0035912)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.33 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2901863

Product Specific Information

Positive Samples: HeLa

Immunogen sequence: RTTLC

Target Information

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin, and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between GSG2 and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AIM-1; ARK-1; ARK-2; ARK-3; Aurora 1; Aurora 2; Aurora 3; Aurora kinase A; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora kinase C; Aurora- and IPL1-like midbody-associated protein 1; aurora-1; aurora-A; aurora-B; aurora-related kinase 1; aurora-related kinase 2; aurora-related kinase 3; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; Aurora/IPL1-related kinase 2; Aurora/IPL1-related kinase 3; Aurora/IPL1/Eg2 protein 2; Breast tumor-amplified kinase; epididymis secretory protein Li 90; Ipl1- and aurora-related kinase 1; protein phosphatase 1, regulatory subunit 47; protein phosphatase 1, regulatory subunit 48; ratAurA; serine/threonine kinase 12; serine/threonine kinase 13 (aurora/IPL1-like); serine/threonine kinase 6; serine/threonine protein kinase 15; serine/threonine protein kinase 6; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; Serine/threonine-protein kinase aurora-B; Serine/threonine-protein kinase aurora-C; Serine/threonine-protein kinase Ayk1; STK-1

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Gene Aliases: AIE2; AIK; AIK2; AIK3; AIM-1; AIM1; AIRK1; AIRK2; AIRK3; ARK-1; ARK-2; ARK1; ARK2; ARK3; AURA; AurB; AurC; AURKA; AURKB; aurkb-sv1; aurkb-sv2; AURKC; aurora-C; AYK1; BTAK; HEL-S-90; IAK1; IPL1; PPP1R47; PPP1R48; SPGF5; STK-1; STK1; STK12; STK13; STK15; STK5; STK6; STK7

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UniProt ID: (Human) O14965, (Human) Q96GD4, (Human) Q9UQB9, (Rat) O55099

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Entrez Gene ID: (Human) 6790, (Human) 9212, (Human) 6795, (Rat) 261730, (Rat) 114592, (Rat) 292554

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding protein kinase binding ubiquitin protein ligase binding histone serine kinase activity metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter negative regulation of B cell apoptotic process protein phosphorylation spindle organization sister chromatid cohesion aging cell proliferation attachment of spindle microtubules to kinetochore abscission histone modification protein sumoylation anaphase-promoting complex-dependent catabolic process spindle checkpoint negative regulation of protein binding positive regulation of telomere maintenance via telomerase negative regulation of cytokinesis positive regulation of cytokinesis protein localization to kinetochore cellular response to UV cleavage furrow formation protein ubiquitination involved in ubiquitin-dependent protein catabolic process histone H3-S28 phosphorylation protein autophosphorylation mitotic spindle midzone assembly positive regulation of telomerase activity regulation of chromosome segregation regulation of signal transduction by p53 class mediator positive regulation of telomere capping meiotic spindle organization microtubule cytoskeleton organization mitotic cell cycle mitotic spindle organization spindle assembly involved in female meiosis I mitotic nuclear division mitotic centrosome separation meiotic nuclear division anterior/posterior axis specification cell projection organization regulation of protein stability response to estradiol positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of cytokinesis histone-serine phosphorylation negative regulation of apoptotic process centrosome organization cell division centrosome localization protein localization to centrosome positive regulation of oocyte maturation neuron projection extension G2/M transition of mitotic cell cycle DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest positive regulation of mitotic nuclear division regulation of centrosome cycle cytokinesis spermatogenesis oocyte development meiotic cell cycle
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