Invitrogen

Phospho-BAD (Ser112) Monoclonal Antibody (G.445.9)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-15085

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50
-

Flow Cytometry (Flow)

1:800
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

G.445.9

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pSer112 of mouse Bad
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

66 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10985837

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

The protein encoded by BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BAD; bcl-2 associated death agonist; Bcl-2-binding component 6; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; Bcl2-antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; bcl2-associated death promoter; BCL2-binding component 6; BCL2-binding protein; Bcl2-L-8; OTTMUSP00000017561; OTTMUSP00000022400

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Gene Aliases: AI325008; BAD; BBC2; BBC6; BCL2L8

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UniProt ID: (Human) Q92934, (Mouse) Q61337, (Rat) O35147

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Entrez Gene ID: (Human) 572, (Mouse) 12015, (Rat) 64639

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Function(s)
protein binding phospholipid binding lipid binding cysteine-type endopeptidase activator activity involved in apoptotic process protein kinase binding protein phosphatase 2B binding protein kinase B binding protein heterodimerization activity 14-3-3 protein binding protein phosphatase binding
Process(es)
release of cytochrome c from mitochondria glucose catabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cell proliferation extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage positive regulation of mitochondrial membrane potential suppression by virus of host apoptotic process cytokine-mediated signaling pathway positive regulation of insulin secretion positive regulation of glucokinase activity positive regulation of insulin secretion involved in cellular response to glucose stimulus glucose homeostasis regulation of apoptotic process positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of cysteine-type endopeptidase activity involved in apoptotic process type B pancreatic cell proliferation positive regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of proteolysis negative regulation of cytolysis ADP metabolic process ATP metabolic process regulation of mitochondrial membrane permeability pore complex assembly positive regulation of epithelial cell proliferation positive regulation of apoptotic process by virus cellular process regulating host cell cycle in response to virus cellular response to organic substance cellular response to nicotine positive regulation of release of cytochrome c from mitochondria apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand intrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity positive regulation of neuron death positive regulation of type B pancreatic cell development response to hypoxia response to hormone response to glucose response to organic substance response to organic cyclic compound response to estradiol response to progesterone response to testosterone response to oleic acid response to drug response to hydrogen peroxide response to amino acid response to ethanol response to glucocorticoid response to calcium ion cellular response to chromate cellular response to mechanical stimulus cellular response to lipid cellular response to hypoxia protein insertion into mitochondrial membrane involved in apoptotic signaling pathway spermatogenesis positive regulation of autophagy cerebral cortex development positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway positive regulation of intrinsic apoptotic signaling pathway
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