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Phospho-BRSK2 (Thr260) Antibody (PA5-105868) in WB
Western blot analysis of Phospho-BRSK2 (Thr260) in EGF treated RAW264.7 cell lysate (absence (-) or presence (+) of non-phospho and phospho peptide). Samples were incubated with Phospho-BRSK2 (Thr260) polyclonal antibody (Product # PA5-105868).
Product Details
PA5-105868
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human BRSK2(Accession Q8IWQ3), corresponding to amino acid residues around phosphorylated Thr260.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2817267
Product Specific Information
Antibody detects endogenous levels of BRSK2 only when phosphorylated at Thr260.
Target Information
BRSK2 is a brain-selective serine/threonine kinase 2 that is mainly expressed in the brain, with weaker expression in testis and pancreas. BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C and CDC25B in an in vitro assay. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhanced endogenous BRSK2 kinase activity in a caffeine-sensitive manner and caused translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 in HeLa cells results in increased phosphorylation of CDC25C. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhances endogenous BRSK2 kinase activity in a caffeine-sensitive manner and causes translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 induces G2/M arrest in HeLa cells. Small interfering RNA against BRSK2 partly abrogates UV-induced G2/M arrest. BRSK2 may act as a checkpoint kinase upon DNA damage induced by UV irradiation or methyl methane sulfonate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BR serine/threonine-protein kinase 2; Brain-selective kinase 2; Brain-specific serine/threonine-protein kinase 2; protein kinase SAD1B; SAD1B; Serine/threonine-protein kinase 29; Serine/threonine-protein kinase BRSK2; Serine/threonine-protein kinase SAD-A; testicular tissue protein Li 30
Gene Aliases: 4833424K13Rik; BRSK2; C11orf7; HUSSY-12; Kiaa4256; PEN11B; SAD-A; SAD1; SADA; STK29
UniProt ID: (Human) Q8IWQ3, (Mouse) Q69Z98
Entrez Gene ID: (Human) 9024, (Mouse) 75770
magnesium ion binding
protein serine/threonine kinase activity
ATP binding
protein kinase binding
tau-protein kinase activity
ATPase binding
ATPase regulator activity
nucleotide binding
protein kinase activity
protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
G2/M transition of mitotic cell cycle
protein phosphorylation
exocytosis
apoptotic process
cell cycle
mitotic nuclear division
nervous system development
axonogenesis
phosphorylation
peptidyl-serine phosphorylation
establishment of cell polarity
neuron differentiation
actin cytoskeleton reorganization
intracellular signal transduction
neuron projection morphogenesis
cell division
regulation of insulin secretion involved in cellular response to glucose stimulus
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
ERAD pathway
regulation of ATPase activity
regulation of retrograde protein transport, ER to cytosol
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